RegulonDB RegulonDB 11.1:Regulon Page

MalI DNA-binding transcriptional repressor

Synonyms: MalI
"Maltose inhibitor," MalI, is a repressor that controls genes related to the maltose system. It is negatively autoregulated and coordinately represses transcription of a divergent operon that encodes a maltose-glucose PTS permease and a bifunctional protein that interacts directly with the MalT transcriptional activator [2, 3] This transcription factor binds to two 12-bp-long direct repeat nucleotide sequences in tandem, overlapping the malXp and malIp simultaneously [2, 3] MalI shows high similarity to GalR (28%), CytR (21%), and LacI (24%), and homology to a fragment of 30 amino acids from MalT. Additionally, the N-terminal region has a helix-turn-helix DNA-binding domain [2] The gene encoding this protein is homologous to transcriptional repressors of the LacI/GalR family.

Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
MalI Functional   nd nd nd
Evolutionary Family: GalR/LacI
TFBs length: 18
TFBs symmetry: inverted-repeat
Connectivity class: Local Regulator
Gene name: malI
  Genome position: 1698152-1699180
  Length: 1029 bp / 342 aa
Operon name: malI
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) malI, malX, malY
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (3)
operon (2)
Transcription related (1)
repressor (1)
Phosphotransferase Systems (PEP-dependent PTS) (1)
Regulated operon(s) malI, malXY
First gene in the operon(s) malI, malX
Simple and complex regulons CRP,MalI
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
  MalI repressor malIp Sigma70 10.5 -38.5 malI
  MalI repressor malXp Sigma70 -16.5 -58.5 malX, malY

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation