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XylR DNA-binding transcriptional dual regulator

Synonyms: XylR, XylR-Xylose
Summary:
Xylose regulator, XylR, is a transcription factor involved in d-xylose degradation. It coregulates with CRP, a global transcriptional regulator [4] These regulators bind cooperatively to activate transcription of operons involved in transport and catabolism of d-xylose. Synthesis of these operons is induced when Escherichia coli is grown on d-xylose in the absence of glucose. Gene induction occurs when the physiological inducer, d-xylose, binds to XylR and when cellular cyclic AMP levels are high [4]
In the presence of d-xylose, XylR binds in tandem to four inverted repeat sequences in the xylAB/xylFGHR intergenic region to activate transcription by overlapping the ?35 boxes of xylABp and xylFGHRp [4] The binding targets for XylR consist of 18-nucleotide-long directed repeat sequences that possess conserved motifs; each monomer binds to one of these conserved sequences [3, 4] XylR belongs to the AraC/XylS family [5, 6] Read more >


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
XylR Non-Functional   Apo [BPP], [GEA] [1]
XylR-Xylose Functional Allosteric Holo [BPP], [GEA] [1]
Evolutionary Family: AraC/XylS
Sensing class: External sensing using transported metabolites
Connectivity class: Local Regulator
Gene name: xylR
  Genome position: 3734979-3736157
  Length: 1179 bp / 392 aa
Operon name: xylFGHR
TU(s) encoding the TF:
Transcription unit        Promoter
xylFGHR
xylFp
xylR
xylRp


Regulon       
Regulated gene(s) araC, xylA, xylB, xylF, xylG, xylH, xylR
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (7)
Transcription related (2)
activator (2)
operon (2)
repressor (1)
Read more >
Regulated operon(s) araC, xylAB, xylFGHR
First gene in the operon(s) araC, xylA, xylF
Simple and complex regulons AraC,CRP,XylR
CRP,Fis,XylR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[XylR,-](1)
[XylR,+](2)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  XylR-Xylose repressor araCp Sigma70 -37.5 -183.5 araC
gtggacttttCTGCCGTGATTATAGACActtttgttac
70195 70212 [AIBSCS], [BPP], [GEA] [2]
  XylR-Xylose repressor araCp Sigma70 -16.5 -162.5 araC
atagacacttTTGTTACGCGTTTTTGTCatggctttgg
70216 70233 [AIBSCS], [BPP], [GEA] [2]
  XylR-Xylose activator xylAp nd -62.5 -104.5 xylA, xylB
agcgcacactTGTGAATTATCTCAATAGcagtgtgaaa
3730861 3730878 [BPP], [GEA], [HIBSCS] [3], [4]
  XylR-Xylose activator xylAp nd -41.5 -83.5 xylA, xylB
tcaatagcagTGTGAAATAACATAATTGagcaactgaa
3730840 3730857 [BPP], [GEA], [HIBSCS] [3], [4]
  XylR-Xylose activator xylFp nd -61.5 -123.5 xylF, xylG, xylH, xylR
tcaagaaataAACCAAAAATCGTAATCGaaagataaaa
3730999 3731016 [BPP], [GEA], [HIBSCS] [3], [4]
  XylR-Xylose activator xylFp nd -40.5 -102.5 xylF, xylG, xylH, xylR
gtaatcgaaaGATAAAAATCTGTAATTGttttcccctg
3731020 3731037 [BPP], [GEA], [HIBSCS] [3], [4]


Alignment and PSSM for XylR TFBSs    

Aligned TFBS of XylR   
  Sequence
  GAAATAACATAATTGA
  GATAATTCACAAGTGT
  CAAAAATCGTAATCGA
  AAAAATCTGTAATTGT

Position weight matrix (PWM).   
A	1	4	3	4	3	2	1	0	2	0	4	4	0	0	0	2
C	1	0	0	0	0	0	1	3	0	1	0	0	0	1	0	0
G	2	0	0	0	0	0	0	0	2	0	0	0	1	0	4	0
T	0	0	1	0	1	2	2	1	0	3	0	0	3	3	0	2

PWM logo   


 


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [HIBSCS] Human inference based on similarity to consensus sequences



Reference(s)    

 [1] Seeger C., Poulsen C., Dandanell G., 1995, Identification and characterization of genes (xapA, xapB, and xapR) involved in xanthosine catabolism in Escherichia coli., J Bacteriol. 177(19):5506-16

 [2] Koirala S., Wang X., Rao CV., null, Reciprocal Regulation of l-Arabinose and d-Xylose Metabolism in Escherichia coli., J Bacteriol. 198(3):386-393

 [3] Laikova ON., Mironov AA., Gelfand MS., 2001, Computational analysis of the transcriptional regulation of pentose utilization systems in the gamma subdivision of Proteobacteria., FEMS Microbiol Lett. 205(2):315-22

 [4] Song S., Park C., 1997, Organization and regulation of the D-xylose operons in Escherichia coli K-12: XylR acts as a transcriptional activator., J Bacteriol. 179(22):7025-32

 [5] Gallegos MT., Schleif R., Bairoch A., Hofmann K., Ramos JL., 1997, Arac/XylS family of transcriptional regulators., Microbiol Mol Biol Rev. 61(4):393-410

 [6] Tobes R., Ramos JL., 2002, AraC-XylS database: a family of positive transcriptional regulators in bacteria., Nucleic Acids Res. 30(1):318-21



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