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CaiF DNA-binding transcriptional activator

Synonyms: CaiF-L-carnitine


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
CaiF-L-carnitine     nd nd
Evolutionary Family: CaiF
Sensing class: External sensing using transported metabolites
Connectivity class: Local Regulator
Gene name: caiF
  Genome position: 34300-34695
  Length: 396 bp / 131 aa
Operon name: caiF
TU(s) encoding the TF:
Transcription unit        Promoter
caiF
caiFp


Regulon       
Regulated gene(s) caiA, caiB, caiC, caiD, caiE, caiT, fixA, fixB, fixC, fixX
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carnitine metabolism (6)
amines (2)
energy production/transport (2)
electron acceptors (1)
carbon compounds (1)
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Regulated operon(s) caiTABCDE, fixABCX
First gene in the operon(s) caiT, fixA
Simple and complex regulons ArcA,CRP,CaiF,FNR,H-NS
CRP,CaiF,FNR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[CaiF,+](2)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  CaiF-L-carnitine activator caiTp Sigma70 -227.5 -333.5 caiT, caiA, caiB, caiC, caiD, caiE
taaatccaacTTTCAATATTGTTAAGttcctcacca
42257 42272 [BPP], [GEA], [HIBSCS] [1]
  CaiF-L-carnitine activator caiTp Sigma70 -208.5 -314.5 caiT, caiA, caiB, caiC, caiD, caiE
tgttaagttcCTCACCAATATTGAAAacacggcgta
42238 42253 [BPP], [GEA], [HIBSCS] [1]
  CaiF-L-carnitine activator caiTp Sigma70 -170.5 -276.5 caiT, caiA, caiB, caiC, caiD, caiE
aaaaagaaatTTTCAATATTGTTTTAtggatcacca
42200 42215 [BPP], [GEA], [HIBSCS] [1]
  CaiF-L-carnitine activator caiTp Sigma70 -151.5 -257.5 caiT, caiA, caiB, caiC, caiD, caiE
tgttttatggATCACCAATATTGAAAgtcagatgtt
42181 42196 [BPP], [GEA], [HIBSCS] [1]
  CaiF-L-carnitine activator fixAp Sigma70 -136.5 -214.5 fixA, fixB, fixC, fixX
aacatctgacTTTCAATATTGGTGATccataaaaca
42181 42196 [BPP], [GEA], [HIBSCS] [1]
  CaiF-L-carnitine activator fixAp Sigma70 -117.5 -195.5 fixA, fixB, fixC, fixX
tggtgatccaTAAAACAATATTGAAAatttcttttt
42200 42215 [BPP], [GEA], [HIBSCS] [1]
  CaiF-L-carnitine activator fixAp Sigma70 -79.5 -157.5 fixA, fixB, fixC, fixX
tacgccgtgtTTTCAATATTGGTGAGgaacttaaca
42238 42253 [BPP], [GEA], [HIBSCS] [1]
  CaiF-L-carnitine activator fixAp Sigma70 -60.5 -138.5 fixA, fixB, fixC, fixX
tggtgaggaaCTTAACAATATTGAAAgttggattta
42257 42272 [BPP], [GEA], [HIBSCS] [1]


Alignment and PSSM for CaiF TFBSs    

Position weight matrix (PWM).   
A	4	2	0	4	4	0	4	0	0	0	4	4	4	2
C	0	2	4	0	0	0	0	0	0	0	0	0	0	0
G	0	0	0	0	0	0	0	0	0	4	0	0	0	2
T	0	0	0	0	0	4	0	4	4	0	0	0	0	0

PWM logo   


 


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences



Reference(s)    

 [1] Buchet A., Nasser W., Eichler K., Mandrand-Berthelot MA., 1999, Positive co-regulation of the Escherichia coli carnitine pathway cai and fix operons by CRP and the CaiF activator., Mol Microbiol. 34(3):562-75



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