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YiaJ DNA-binding transcriptional repressor

Synonyms: YiaJ
Summary:
YiaJ is a transcriptional repressor that negatively controls the expression of genes involved in the catabolism of the rare pentose l-lyxose |CITS:[10913096][9525947][15870475]|.
YiaJ is negatively autoregulated, and coordinately represses transcription of the divergent yiaK-S operon, related to degradation of l-lyxose |CITS:[10913096]|. Additionally, its operon is activated by insertion of IS1 into the yiaJ gene |CITS:[9525947]|. Although little is known about the mechanism of regulation of the YiaJ regulator, recently it has been demonstrated to act as a repressor by binding to cis-acting elements that overlap yiaKp |CITS:[10913096]|.
YiaJ shows high homology to the IclR family of regulators; it is composed of two domains, an amino-terminal domain that contains a potential helix-turn-helix DNA-binding motif and a carboxy-terminal region featuring a second motif of unknown function |CITS:[9525947]|.


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
YiaJ     nd nd
Evolutionary Family: IclR
Connectivity class: Local Regulator
Gene name: yiaJ
  Genome position: 3741684-3742532
  Length: 849 bp / 282 aa
Operon name: yiaJ
TU(s) encoding the TF:
Transcription unit        Promoter
yiaJ
yiaJp


Regulon       
Regulated gene(s) lyxK, sgbE, sgbH, sgbU, yiaJ, yiaK, yiaL, yiaM, yiaN, yiaO
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (4)
Porters (Uni-, Sym- and Antiporters) (3)
membrane (2)
unassigned reversible reactions (1)
Transcription related (1)
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Regulated operon(s) yiaJ, yiaKLMNO-lyxK-sgbHUE
First gene in the operon(s) yiaJ, yiaK
Simple and complex regulons CRP,IHF,YiaJ
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[YiaJ,-](2)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  YiaJ repressor yiaJp Sigma70 -55.0 -141.0 yiaJ
aaatttcaatATGCGAAACTTGATTTCAAATatatcgatca
3742663 3742683 [BCE], [GEA] [1]
  YiaJ repressor yiaKp Sigma70 -1.0 -60.0 yiaK, yiaL, yiaM, yiaN, yiaO, lyxK, sgbH, sgbU, sgbE
tgatcgatatATTTGAAATCAAGTTTCGCATattgaaattt
3742663 3742683 [BCE], [GEA] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BCE] Binding of cellular extracts

 [GEA] Gene expression analysis



Reference(s)    

 [1] Ibanez E., Campos E., Baldoma L., Aguilar J., Badia J., 2000, Regulation of expression of the yiaKLMNOPQRS operon for carbohydrate utilization in Escherichia coli: involvement of the main transcriptional factors., J Bacteriol. 182(16):4617-24



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