AdiY is a positive DNA-binding transcriptional regulator that controls the arginine decarboxylase (adi) system, which is strongly induced in rich medium, under anaerobic conditions, and at low pH [2, 3].
Several proteins are involved in the induction of the adi system. Changes in the external pH activate the adiA gene. AdiY may function directly or indirectly with CysB to increase expression of adiA. IHF and H-NS might modulate the bending and necessary access of the adiAp promoter for activation . It has also been proposed that AdiY is not the only regulator of the adi system, but the conditions under which AdiY functions remain unknown .
The amino acid sequence of AdiY shows homology to members of the XylS/AraC family of transcriptional regulators [2, 3]. The C-terminal region of proteins from this family features a helix-turn-helix motif. AdiY has a putative transmembrane region, suggesting that it may be a membrane-associated protein .
The gene adiY is located downstream of adiA and in the same orientation .
The lack of an evident transcription-termination structure in the intergenic region between adiY and adiC, separated by 137 bp, suggests the possibility of both genes being part of the same operon , but further studies have failed to confirm this .Read more >
Translation of AdiY is repressed by the small RNA SgrS; overexpression of AdiY exacerbates the effects of glucose-phosphate stress in a ppsA mutant .
|Connectivity class:||Local Regulator|
|Length:||762 bp / 253 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||adiA, gadA, gadB, gadC, gadX, gltB, gltD, gltF|
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
adiA, gadA, gadB
nitrogen metabolism (2)
Porters (Uni-, Sym- and Antiporters) (1)
gadCRead more >
|Regulated operon(s)||adiA, gadAXW, gadBC, gltBDF|
|First gene in the operon(s)||adiA, gadA, gadB, gltB|
|Simple and complex regulons|
|Simple and complex regulatory phrases||Regulatory phrase (List of promoters regulated by the phrase)|
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|AdiY||activator||adiAp||Sigma70||nd||nd||adiA||nd||nd||[BPP], [GEA]||, |
|AdiY||activator||gltBp||Sigma70||nd||nd||gltB, gltD, gltF||nd||nd||[BPP]|||
|Evolutionary conservation of regulatory elements|
 Stim-Herndon KP., Flores TM., Bennett GN., 1996, Molecular characterization of adiY, a regulatory gene which affects expression of the biodegradative acid-induced arginine decarboxylase gene (adiA) of Escherichia coli., Microbiology. 142 ( Pt 5):1311-20
 Stim-Herndon KP., 1995, Effect of the local anesthetics phenethyl alcohol and procaine on hns mutants of the acid-induced biodegradative arginine (adi) and lysine (cad) decarboxylases of Escherichia coli., Curr Microbiol. 30(5):281-5