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AllS DNA-binding transcriptional activator

Synonyms: AllS-allantoin, AllS
Summary:
AllS, allantoin , transcriptional activator participates in controlling genes involved in the utilization of allantoin as a nitrogen source. Although AllS binding does not depend on the presence of allantoin, this compound appears to be necessary for activation, perhaps by interacting with AllS [1]
AllS belongs to the LysR family of transcriptional regulators, and like other members of this family, it has a putative helix-turn-helix motif in the N-terminal domain [1]
Expression of the allS gene is negligible under aerobic conditions and is increased under anaerobic conditions in the presence of glyoxylate or allantoin. In the absence of its repressor, AllR, allS is expressed under nitrogen-limiting growth conditions, but its induction is independent of the regulators Nac and NtrC, which are active under these conditions [1]


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
AllS Non-Functional   Apo [BPP], [GEA] [1]
AllS-allantoin Functional Allosteric Holo [BPP], [GEA] [1]
Evolutionary Family: LysR
Sensing class: Sensing external and internal signals
Connectivity class: Local Regulator
Gene name: allS
  Genome position: 531295-532221
  Length: 927 bp / 308 aa
Operon name: allS
TU(s) encoding the TF:
Transcription unit        Promoter
allS
allSp


Regulon       
Regulated gene(s) allC, allD, allE
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
allantoin assimilation (3)
nitrogen metabolism (3)
Regulated operon(s) allDCE
First gene in the operon(s) allD
Simple and complex regulons AllS
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[AllS,+](1)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  AllS-allantoin activator allDp Sigma70 -94.0 -142.0 allD, allC, allE
ttaataagcgTTGTTGTTTTAAAAAtcaggttaaa
546498 546512 [BCE], [HIBSCS] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [BCE] Binding of cellular extracts

 [HIBSCS] Human inference based on similarity to consensus sequences



Reference(s)    

 [1] Rintoul MR., Cusa E., Baldoma L., Badia J., Reitzer L., Aguilar J., 2002, Regulation of the Escherichia coli allantoin regulon: coordinated function of the repressor AllR and the activator AllS., J Mol Biol. 324(4):599-610



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