|Synonyms: DhaR, DhaR-DhaK|
Dihydroxyacetone Regulator, DhaR, is negatively autoregulated and coordinately activates transcription of the divergent dha operon (dhaKLM), which encodes three subunits of the dihydroxyacetone (DHA) kinase .
DhaR is inactivated when DnaK binds to the sensing domain of this regulator, in the absence of DHA . Dephosphorylated DhaL (DhaL::ADP) is an antagonist of DhaK and also is able to form complexes with the sensing domain of DhaR. In the presence of DHA, DhaL::ADP displaces DhaK or blocks the association of the DhaK/DhaR complex and DhaR activates the transcription of the dha operon .
DhaR belongs to the family of bacterial enhancer-binding proteins which contain three domains: the sensing domain located in the N terminus, the central AAA+ domain, and the C-terminal domain, which contains a helix-turn-helix motif involved in the interaction with DNA. Although the C-terminal domain and the N-terminal domain from DhaR are similar to those of other members of this group, its central domain is not .
The central AAA+ domain does not contain the two conserved submotifs in this family (ESELF and GAFTGA) responsible for interaction with σ54. As with other members of the family, the possibility exists that this regulator activates σ70-dependent promoters instead of σ54-dependent promoters .Read more >
The crystal structure has been determined. DhaR regulates transcription by coiled-coil helix rotation, and this provides the mechanism for transmitting the binding signal from the GAF/PAS domains to the C-terminal DNA-binding domain .
|Connectivity class:||Local Regulator|
|Length:||1920 bp / 639 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||dhaK, dhaL, dhaM, dhaR|
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
carbon compounds (3)
dhaK, dhaL, dhaM
Transcription related (1)
two component regulatory systems (external signal) (1)
|Regulated operon(s)||dhaKLM, dhaR|
|First gene in the operon(s)||dhaK, dhaR|
|Simple and complex regulons|
|Simple and complex regulatory phrases||Regulatory phrase (List of promoters regulated by the phrase)|
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|DhaR||activator||dhaKp||Sigma38||nd||nd||dhaK, dhaL, dhaM||nd||nd||[GEA]|||
|Evolutionary conservation of regulatory elements|