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Synonyms: AcrR-ethidium, AcrR, AcrR-Proflavin, AcrR-R6G |
Summary:
The "acriflavine resistance regulator," AcrR, regulates the expression of genes involved in multidrug transport and is classified in the nodulation-resistance division family [3]. It also acts as a global repressor for the mar-sox-rob regulon [2]. The crystal structure of AcrR shows that this protein is folded into nine α-helices distributed in two domains: the DNA-binding domain, which forms the typical helix-turn-helix in the N-terminal domain, and the ligand-binding domain, which forms a large internal cavity for multidrug binding in the C-terminal domain [6]. Mutational analyses have been used to identify the critical role of some amino acids for AcrR activity, such as Glu-67 [6] and Arg-45 [7]. The activity of AcrR, which belongs to the TetR family, appears to be regulated by ligands such as rhodamine 6G, ethidium, and proflavin [1]. Read more > |
Transcription factor | ![]() ![]() |
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TF conformation(s): |
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Evolutionary Family: | TetR/AcrR | ||||||||||||||||||||||||||||||
Connectivity class: | Local Regulator | ||||||||||||||||||||||||||||||
Gene name: | acrR | ||||||||||||||||||||||||||||||
Genome position: | 485761-486408 | ||||||||||||||||||||||||||||||
Length: | 648 bp / 215 aa | ||||||||||||||||||||||||||||||
Operon name: | acrR | ||||||||||||||||||||||||||||||
TU(s) encoding the TF: |
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Regulon |
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Regulated gene(s) | acrA, acrB, acrR, flhC, flhD, marA, marB, marR, micF, soxR, soxS | ||||||
Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
drug resistance/sensitivity (7)
Transcription related (7)
repressor (7)
operon (6)
activator (5)
Read more >
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Regulated operon(s) | acrAB, acrR, flhDC, marRAB, micF, soxR, soxS | ||||||
First gene in the operon(s) | acrA, acrR, flhD, marR, micF, soxR, soxS | ||||||
Simple and complex regulons | AcrR AcrR,CRP,CpxR,Cra,Fis,MarA,MarR,Rob,SoxS AcrR,CRP,FliZ,Fur,H-NS,HdfR,IHF,LrhA,MatA,OmpR,QseB,RcsAB,YjjQ AcrR,EnvR,MarA,MprA,PhoP,Rob,SoxS AcrR,FNR,Fur,SoxR Read more > | ||||||
Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) |
Transcription factor regulation |
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Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence (Confirmed, Strong, Weak) | References | |
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AcrR | repressor | acrAp | Sigma70 | -22.5 | -101.5 | acrA, acrB |
gcgttagattTACATACATTTGTGAATGTATGTAccatagcacg
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485709 | 485732 | [GEA], [AIBSCS], [BPP] | [1], [2], [3] | |
AcrR | repressor | acrRp | Sigma70 | 22.5 | -40.5 | acrR |
cgtgctatggTACATACATTCACAAATGTATGTAaatctaacgc
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485709 | 485732 | [AIBSCS], [APIORCISFBSCS], [BCE], [BPP], [GEA], [AIBSCS], [BPP] | [1], [2], [3], [4] | |
AcrR | repressor | flhDp | Sigma70 | -31.5 | -229.5 | flhD, flhC |
agcccccctcCGTTGTATGTGcgtgtagtga
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1978422 | 1978432 | [GEA], [APIORCISFBSCS] | [5] | |
AcrR | repressor | marRp | Sigma70 | -40.5 | -67.5 | marR, marA, marB |
catcggtcaaTTCATTCATTTgacttatact
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1619048 | 1619058 | [GEA], [APIORCISFBSCS], [BPP] | [2] | |
AcrR | repressor | micFp1 | Sigma70 | 41.0 | 41.0 | micF |
atttattaccGTCATTCATTTCTGAATGTCTGTTtacccctatt
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2313112 | 2313135 | [AIBSCS] | [4] | |
AcrR | repressor | micFp2 | Sigma38 | 41.0 | 41.0 | micF |
atttattaccGTCATTCATTTCTGAATGTCTGTTtacccctatt
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2313112 | 2313135 | [AIBSCS] | [4] | |
AcrR | repressor | soxRp | Sigma70 | 2.5 | -20.5 | soxR |
ataattcctcAAGTTAACTTGaggtaaagcg
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4277444 | 4277454 | [GEA], [APIORCISFBSCS], [BPP] | [2] | |
AcrR | repressor | soxSp | Sigma70 | -25.5 | -65.5 | soxS |
cgctttacctCAAGTTAACTTgaggaattat
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4277444 | 4277454 | [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP] | [2] |
Alignment and PSSM for AcrR TFBSs | ![]() |
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Aligned TFBS of AcrR |
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Sequence | |
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TACATACATTTGTG | |
TTCATTCATTTGAC | |
GTCATTCATTTCTG | |
CACATACAACGGAG | |
CAAGTTAACTTGAG |
Position weight matrix (PWM). AcrR matrix-quality result |
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A 0 3 1 4 0 2 1 5 1 0 0 0 3 0 C 2 0 4 0 0 0 4 0 1 1 0 1 0 1 G 1 0 0 1 0 0 0 0 0 0 1 4 0 4 T 2 2 0 0 5 3 0 0 3 4 4 0 2 0 |
Consensus |
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; consensus.strict caCaTtCAtttGaG ; consensus.strict.rc CTCAAATGAATGTG ; consensus.IUPAC ywCaTwCAtttGwG ; consensus.IUPAC.rc CWCAAATGWATGWR ; consensus.regexp [ct][at]CaT[at]CAtttG[at]G ; consensus.regexp.rc C[AT]CAAATG[AT]ATG[AT][AG] |
Evolutionary conservation of regulatory elements | ![]() |
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Reference(s) |
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