RegulonDB RegulonDB 10.8:Regulon Page

AcrR DNA-binding transcriptional repressor

Synonyms: AcrR-Proflavin, AcrR-R6G, AcrR, AcrR-ethidium
The "acriflavine resistance regulator," AcrR, regulates the expression of genes involved in multidrug transport and is classified in the nodulation-resistance division family [1]. It also acts as a global repressor for the mar-sox-rob regulon [3] The crystal structure of AcrR shows that this protein is folded into nine α-helices distributed in two domains: the DNA-binding domain, which forms the typical helix-turn-helix in the N-terminal domain, and the ligand-binding domain, which forms a large internal cavity for multidrug binding in the C-terminal domain []. Mutational analyses have been used to identify the critical role of some amino acids for AcrR activity, such as Glu-67 [] and Arg-45 []. The activity of AcrR, which belongs to the TetR family, appears to be regulated by ligands such as rhodamine 6G, ethidium, and proflavin [2].
Read more >

Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
AcrR Functional   Apo [APPH], [BPP], [GEA], [IEP], [IMP], [IPI] [1], [2]
AcrR-Proflavin Non-Functional Allosteric Holo [BPP], [IPI] [2]
AcrR-R6G Non-Functional Allosteric Holo [BPP], [IPI] [2]
AcrR-ethidium Non-Functional Allosteric Holo [BPP], [IPI] [2]
Evolutionary Family: TetR/AcrR
Connectivity class: Local Regulator
Gene name: acrR
  Genome position: 485761-486408
  Length: 648 bp / 215 aa
Operon name: acrR
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) acrA, acrB, acrR, flhC, flhD, marA, marB, marR, micF, soxR, soxS
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
drug resistance/sensitivity (7)
Transcription related (7)
repressor (7)
operon (6)
activator (5)
Read more >
Regulated operon(s) acrAB, acrR, flhDC, marRAB, micF, soxR, soxS
First gene in the operon(s) acrA, acrR, flhD, marR, micF, soxR, soxS
Simple and complex regulons AcrR
Read more >
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  AcrR repressor acrAp Sigma70 -22.5 -101.5 acrA, acrB
485709 485732 [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1], [2], [3]
  AcrR repressor acrRp Sigma70 22.5 -40.5 acrR
485709 485732 [AIBSCS], [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1], [2], [3], [4]
  AcrR repressor flhDp Sigma70 -31.5 -229.5 flhD, flhC
1978422 1978432 [APIORCISFBSCS], [CV(GEA)], [GEA] [5]
  AcrR repressor marRp Sigma70 -40.5 -67.5 marR, marA, marB
1619048 1619058 [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [3]
  AcrR repressor micFp Sigma38, Sigma70 41.0 41.0 micF
2313112 2313135 [AIBSCS] [4]
  AcrR repressor soxRp Sigma70 2.5 -20.5 soxR
4277444 4277454 [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [3]
  AcrR repressor soxSp Sigma70 -25.5 -65.5 soxS
4277444 4277454 [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [3]

Alignment and PSSM for AcrR TFBSs    

Aligned TFBS of AcrR   

Position weight matrix (PWM). AcrR matrix-quality result   
A	0	3	1	4	0	2	1	5	1	0	0	0	3	0
C	2	0	4	0	0	0	4	0	1	1	0	1	0	1
G	1	0	0	1	0	0	0	0	0	0	1	4	0	4
T	2	2	0	0	5	3	0	0	3	4	4	0	2	0

;	consensus.strict             	caCaTtCAtttGaG
;	consensus.strict.rc          	CTCAAATGAATGTG
;	consensus.IUPAC              	ywCaTwCAtttGwG
;	consensus.IUPAC.rc           	CWCAAATGWATGWR
;	consensus.regexp             	[ct][at]CaT[at]CAtttG[at]G
;	consensus.regexp.rc          	C[AT]CAAATG[AT]ATG[AT][AG]

PWM logo   


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [APPH] Assay of protein purified to homogeneity

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [IEP] Inferred from expression pattern

 [IMP] Inferred from mutant phenotype

 [IPI] Inferred from physical interaction

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BCE] Binding of cellular extracts

 [CV(GEA)] cross validation(GEA)

 [AIBSCS] Automated inference based on similarity to consensus sequences


 [1] Ma D., Alberti M., Lynch C., Nikaido H., Hearst JE., 1996, The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals., Mol Microbiol 19(1):101-12

 [2] Su CC., Rutherford DJ., Yu EW., 2007, Characterization of the multidrug efflux regulator AcrR from Escherichia coli., Biochem Biophys Res Commun 361(1):85-90

 [3] Lee JO., Cho KS., Kim OB., 2014, Overproduction of AcrR increases organic solvent tolerance mediated by modulation of SoxS regulon in Escherichia coli., Appl Microbiol Biotechnol 98(20):8763-73

 [4] Rodionov DA., Gelfand MS., Mironov AA., Rakhmaninova AB., 2001, Comparative approach to analysis of regulation in complete genomes: multidrug resistance systems in gamma-proteobacteria., J Mol Microbiol Biotechnol 3(2):319-24

 [5] Kim YJ., Im SY., Lee JO., Kim OB., 2016, Potential Swimming Motility Variation by AcrR in Escherichia coli., J Microbiol Biotechnol 26(10):1824-1828