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PepA DNA-binding transcriptional repressor

Synonyms: PepA


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
PepA     nd nd
Evolutionary Family: Aminopeptidase
Connectivity class: Local Regulator
Gene name: pepA
  Genome position: 4484440-4485951
  Length: 1512 bp / 503 aa
Operon name: pepA
TU(s) encoding the TF:
Transcription unit        Promoter
pepA
pepAp1
pepA
pepAp2
pepA
pepAp3


Regulon       
Regulated gene(s) carA, carB, pepA
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
arginine (2)
pyrimidine biosynthesis (2)
proteins/peptides/glycopeptides (1)
DNA recombination (1)
Transcription related (1)
Regulated operon(s) carAB, pepA
First gene in the operon(s) carA, pepA
Simple and complex regulons Fis,IHF,PepA,PurR,RutR
PepA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[PepA,-](2)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  PepA repressor carAp1 Sigma70 -173.0 -273.0 carA, carB
aattttgaccATTTGGTCCACTTTTTTCTGCTCGTTTttatttcatg
29365 29391 [BPP], [GEA], [IMP] [1], [2], [3]
  PepA repressor carAp1 Sigma70 -79.0 -179.0 carA, carB
ttttgtcgctTAATGCCTGTAAAACATGCATGAGCCAcaaaataata
29459 29485 [BPP], [GEA] [1], [2], [3]
  PepA repressor pepAp1 Sigma70 -43.0 -231.0 pepA
cgtaaaaactCGTCTTTTGCAGGATTTTAGCTTGTTTcatggcttaa
4486169 4486195 [BPP] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [IMP] Inferred from mutant phenotype



Reference(s)    

 [1] Charlier D., Hassanzadeh G., Kholti A., Gigot D., Pierard A., Glansdorff N., 1995, carP, involved in pyrimidine regulation of the Escherichia coli carbamoylphosphate synthetase operon encodes a sequence-specific DNA-binding protein identical to XerB and PepA, also required for resolution of ColEI multimers., J Mol Biol. 250(4):392-406

 [2] Devroede N., Huysveld N., Charlier D., 2006, Mutational analysis of intervening sequences connecting the binding sites for integration host factor, PepA, PurR, and RNA polymerase in the control region of the Escherichia coli carAB operon, encoding carbamoylphosphate synthase., J Bacteriol. 188(9):3236-45

 [3] Devroede N., Thia-Toong TL., Gigot D., Maes D., Charlier D., 2004, Purine and pyrimidine-specific repression of the Escherichia coli carAB operon are functionally and structurally coupled., J Mol Biol. 336(1):25-42



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