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PepA DNA-binding transcriptional repressor

Synonyms: PepA
Summary:
Aminopeptidase A/I is a Peptidase that binds DNA and controls the transcription of the operon involved in the synthesis of carbamoyl phosphate, which is an intermediate related to the pyrimidine nucleotide pathway [1, 2, 3]. This protein also is required for maintenance of plasmid monomers, which is critical for proper plasmid segregation [4]. Aminopeptidase A/I is a part of the site-specific recombination system required to decatenate the plasmids ColE1 and pSC101, maintaining them as monomers []. Aminopeptidase A/I is responsible for coordinating DNA strands during recombination, pairing cer sites in ColE1 plasmids and allowing the formation of Holliday junctions []. Aminopeptidase A/I, along with ArgR, may be involved in blocking trans-recombination, thus preventing the formation of new catenated plasmids during recombination [].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
PepA Functional   nd [1]
Evolutionary Family: Aminopeptidase
Connectivity class: Local Regulator
Gene name: pepA
  Genome position: 4484440-4485951
  Length: 1512 bp / 503 aa
Operon name: pepA
TU(s) encoding the TF:
Transcription unit        Promoter
pepA
pepAp1
pepA
pepAp2
pepA
pepAp3


Regulon       
Regulated gene(s) carA, carB, pepA
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
arginine (2)
pyrimidine biosynthesis (2)
proteins/peptides/glycopeptides (1)
DNA recombination (1)
Transcription related (1)
Regulated operon(s) carAB, pepA
First gene in the operon(s) carA, pepA
Simple and complex regulons Fis,IHF,PepA,PurR,RutR
PepA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[PepA,-](2)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  PepA repressor carAp1 Sigma70 -173.0 -273.0 carA, carB
aattttgaccATTTGGTCCACTTTTTTCTGCTCGTTTttatttcatg
 29365 29391 [BPP], [CV(GEA)], [CV(GEA)], [GEA], [IMP] [1], [2], [3]
  PepA repressor carAp1 Sigma70 -79.0 -179.0 carA, carB
ttttgtcgctTAATGCCTGTAAAACATGCATGAGCCAcaaaataata
 29459 29485 [BPP], [GEA] [1], [2], [3]
  PepA repressor pepAp1 Sigma70 -43.0 -231.0 pepA
cgtaaaaactCGTCTTTTGCAGGATTTTAGCTTGTTTcatggcttaa
 4486169 4486195 [BPP] [2]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [IMP] Inferred from mutant phenotype


Reference(s)    

 [1] Devroede N., Thia-Toong TL., Gigot D., Maes D., Charlier D., 2004, Purine and pyrimidine-specific repression of the Escherichia coli carAB operon are functionally and structurally coupled., J Mol Biol 336(1):25-42

 [2] Charlier D., Hassanzadeh G., Kholti A., Gigot D., Pierard A., Glansdorff N., 1995, carP, involved in pyrimidine regulation of the Escherichia coli carbamoylphosphate synthetase operon encodes a sequence-specific DNA-binding protein identical to XerB and PepA, also required for resolution of ColEI multimers., J Mol Biol 250(4):392-406

 [3] Devroede N., Huysveld N., Charlier D., 2006, Mutational analysis of intervening sequences connecting the binding sites for integration host factor, PepA, PurR, and RNA polymerase in the control region of the Escherichia coli carAB operon, encoding carbamoylphosphate synthase., J Bacteriol 188(9):3236-45

 [4] Miller CG., Schwartz G., 1978, Peptidase-deficient mutants of Escherichia coli., J Bacteriol 135(2):603-11

 [5] Charlier D., Gigot D., Huysveld N., Roovers M., Pierard A., Glansdorff N., 1995, Pyrimidine regulation of the Escherichia coli and Salmonella typhimurium carAB operons: CarP and integration host factor (IHF) modulate the methylation status of a GATC site present in the control region., J Mol Biol 250(4):383-91


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