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Synonyms: PepA |
Summary:
Aminopeptidase A/I is a Peptidase that binds DNA and controls the transcription of the operon involved in the synthesis of carbamoyl phosphate, which is an intermediate related to the pyrimidine nucleotide pathway [1, 2, 3, 4]. This protein also is required for maintenance of plasmid monomers, which is critical for proper plasmid segregation [4, 5, 6, 7, 8]. Aminopeptidase A/I is a part of the site-specific recombination system required to decatenate the plasmids ColE1 and pSC101, maintaining them as monomers [6, 9, 10]. Aminopeptidase A/I is responsible for coordinating DNA strands during recombination, pairing cer sites in ColE1 plasmids and allowing the formation of Holliday junctions [11, 12, 13]. Aminopeptidase A/I, along with ArgR, may be involved in blocking trans-recombination, thus preventing the formation of new catenated plasmids during recombination [14]. Read more > |
Transcription factor | ![]() ![]() |
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TF conformation(s): |
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Evolutionary Family: | Aminopeptidase | ||||||||||||||
TFBs length: | 27 | ||||||||||||||
TFBs symmetry: | inverted-repeat | ||||||||||||||
Connectivity class: | Local Regulator | ||||||||||||||
Gene name: | pepA | ||||||||||||||
Genome position: | 4484440-4485951 | ||||||||||||||
Length: | 1512 bp / 503 aa | ||||||||||||||
Operon name: | pepA | ||||||||||||||
TU(s) encoding the TF: |
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Regulon |
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Regulated gene(s) | carA, carB, pepA | ||||||
Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
arginine (2)
pyrimidine biosynthesis (2)
proteins/peptides/glycopeptides (1)
DNA recombination (1)
Transcription related (1)
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Regulated operon(s) | carAB, pepA | ||||||
First gene in the operon(s) | carA, pepA | ||||||
Simple and complex regulons | Fis,IHF,PepA,PurR,RutR PepA | ||||||
Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) |
Transcription factor regulation |
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Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | References | |
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PepA | repressor | carAp1 | Sigma70 | -173.0 | -273.0 | carA, carB |
aattttgaccATTTGGTCCACTTTTTTCTGCTCGTTTttatttcatg
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29365 | 29391 | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] | S | [1], [2], [2], [3] | |
PepA | repressor | carAp1 | Sigma70 | -79.0 | -179.0 | carA, carB |
ttttgtcgctTAATGCCTGTAAAACATGCATGAGCCAcaaaataata
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29459 | 29485 | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] | S | [1], [2], [2], [3] | |
PepA | repressor | pepAp1 | Sigma70 | -43.0 | -231.0 | pepA |
cgtaaaaactCGTCTTTTGCAGGATTTTAGCTTGTTTcatggcttaa
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4486169 | 4486195 | [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] | S | [3], [3] |
Evolutionary conservation of regulatory elements | ![]() |
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Reference(s) |
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