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AscG DNA-binding transcriptional repressor

Synonyms: AscG
Summary:
The AscG, Arbutin-salicin-cellibiose, transcriptional regulator represses the expression of a cryptic operon (ascFB) whose genes are involved in transport and utilization of the β-glucoside sugars arbutin, salicin, and cellibiose. This operon is activated only when the repressor is inactivated through the interruption of the gene by an insertion sequence [1] AscG also regulates prpBC for propionate catabolism [2]
AscG is a GalR-type transcription factor and has a helix-turn-helix motif [1, 2] AscG recognizes and binds the consensus palindromic sequence TGAAACCGGTTTCA [2]
The ascG gene is transcribed divergently from the operon it regulates [1] ascG is paralogous to galR [2] A high level of AscG is always present in wild-type Escherichia coli cells [2]
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
AscG     nd nd
Evolutionary Family: GalR/LacI
Sensing class: External sensing using transported metabolites
Connectivity class: Local Regulator
Gene name: ascG
  Genome position: 2838254-2839264
  Length: 1011 bp / 336 aa
Operon name: ascG
TU(s) encoding the TF:
Transcription unit        Promoter
ascG
null


Regulon       
Regulated gene(s) ascB, ascF, htpG, prpR, ybiT
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (2)
Phosphotransferase Systems (PEP-dependent PTS) (1)
membrane (1)
chaperoning, repair (refolding) (1)
fatty acids (1)
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Regulated operon(s) ascFB, htpG, prpR, ybiT
First gene in the operon(s) ascF, htpG, prpR, ybiT
Simple and complex regulons AscG
AscG,CRP
AscG,CRP,PhoB,PrpR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[AscG,-](4)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
 LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  AscG repressor ascFp Sigma70 -72.5 -167.5 ascF, ascB
aaaatattcaGGTGACCGGTTTCAcaaatataaa
 2839350 2839363 [AIBSCS], [BPP], [GEA] [1], [2]
  AscG repressor ascFp Sigma70 4.5 -91.5 ascF, ascB
catgattttgTGAAACCGGTTTCTtaattccgtt
 2839426 2839439 [AIBSCS], [BPP], [GEA] [2]
  AscG repressor htpGp1 Sigma32 89.5 43.5 htpG
cagtcagaagTGAAACAGCTTCTGcacctgatga
 495157 495170 [AIBSCS], [BPP] [2]
  AscG repressor prpRp Sigma70 -58.5 -86.5 prpR
ttcagttaacGTTTCAggcaatgttt
 348527 348532 [AIBSCS], [BPP], [GEA] [2]
  AscG repressor prpRp Sigma70 -46.5 -74.5 prpR
ttcaggcaatGTTTCAccgcgtttca
 348515 348520 [AIBSCS], [BPP], [GEA] [2]
  AscG repressor prpRp Sigma70 -36.5 -64.5 prpR
gtttcaccgcGTTTCAttgcaacaat
 348505 348510 [AIBSCS], [BPP], [GEA] [2]
  AscG repressor prpRp Sigma70 -29.5 -57.5 prpR
cgcgtttcatTGCAACaattatgaaa
 348498 348503 [AIBSCS], [BPP], [GEA] [2]
  AscG repressor prpRp Sigma70 -18.5 -46.5 prpR
gcaacaattaTGAAACaagactaaac
 348487 348492 [AIBSCS], [BPP], [GEA] [2]
  AscG repressor ybiTp nd -108.5 -124.5 ybiT
cagctcacagTGAAATCAGATGTGtacgaaatca
 855832 855845 [AIBSCS], [BPP] [2]


Alignment and PSSM for AscG TFBSs    

Aligned TFBS of AscG   
  Sequence
  ATGAAACAAGAC
  TTGCAACAATTA
  ATGAAACGCGGT
  GTGAAACATTGC
  CTGAAACGTTAA
  GTGAAACAGCTT
  GTGAAATCAGAT
  GTGAAACCGGTC
  GTGAAACCGGTT

Position weight matrix (PWM).    
A	2	0	0	8	9	9	0	4	3	0	3	2
C	1	0	0	1	0	0	8	3	1	1	0	3
G	5	0	9	0	0	0	0	2	3	5	2	0
T	1	9	0	0	0	0	1	0	2	3	4	4

PWM logo   


 


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
TF-target gene evolutionary conservation
Promoter-target gene evolutionary conservation


Evidence    

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis


Reference(s)    

 [1] Hall BG., Xu L., 1992, Nucleotide sequence, function, activation, and evolution of the cryptic asc operon of Escherichia coli K12., Mol Biol Evol. 9(4):688-706

 [2] Ishida Y., Kori A., Ishihama A., 2009, Participation of regulator AscG of the beta-glucoside utilization operon in regulation of the propionate catabolism operon., J Bacteriol. 191(19):6136-44


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