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| Synonyms: AscG |
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Summary:
The AscG, "Arbutin-salicin-cellibiose," transcriptional regulator represses the expression of a cryptic operon (ascFB) whose genes are involved in transport and utilization of the β-glucoside sugars arbutin, salicin, and cellibiose. This operon is activated only when the repressor is inactivated through the interruption of the gene by an insertion sequence [1] AscG also regulates prpBC for propionate catabolism [2] AscG is a GalR-type transcription factor and has a helix-turn-helix motif [1, 2] AscG recognizes and binds the consensus palindromic sequence TGAAACCGGTTTCA [2] The ascG gene is transcribed divergently from the operon it regulates [1] ascG is paralogous to galR [2] A high level of AscG is always present in wild-type Escherichia coli cells [2] Read more > |
| Transcription factor |  |
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| TF conformation(s): |
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| Evolutionary Family: | GalR/LacI | ||||||||||||
| Sensing class: | External sensing using transported metabolites | ||||||||||||
| Connectivity class: | Local Regulator | ||||||||||||
| Gene name: | ascG | ||||||||||||
| Genome position: | 2838254-2839264 | ||||||||||||
| Length: | 1011 bp / 336 aa | ||||||||||||
| Operon name: | ascG | ||||||||||||
| TU(s) encoding the TF: |
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| Regulon |
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| Regulated gene(s) | ascB, ascF, htpG, prpR, ybiT | ||||||
| Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
carbon compounds (2)
Phosphotransferase Systems (PEP-dependent PTS) (1)
membrane (1)
chaperoning, repair (refolding) (1)
fatty acids (1)
Read more >
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| Regulated operon(s) | ascFB, htpG, prpR, ybiT | ||||||
| First gene in the operon(s) | ascF, htpG, prpR, ybiT | ||||||
| Simple and complex regulons | AscG AscG,CRP AscG,CRP,PhoB,PrpR | ||||||
| Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) | ||||||
| Transcription factor regulation |
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Transcription factor binding sites (TFBSs) arrangements
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| Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence |
LeftPos | RightPos | Evidence (Confirmed, Strong, Weak) | References | |
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| AscG | repressor | ascFp | Sigma70 | -72.5 | -167.0 | ascF, ascB |
aaaatattcaGGTGACCGGTTTCACaaatataaaa
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2839350 | 2839364 | [AIBSCS], [BPP], [GEA] | [1], [2] | |
| AscG | repressor | ascFp | Sigma70 | 4.5 | -91.0 | ascF, ascB |
catgattttgTGAAACCGGTTTCTTaattccgttt
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2839426 | 2839440 | [AIBSCS], [BPP], [GEA] | [2] | |
| AscG | repressor | htpGp1 | Sigma32 | 89.5 | 44.0 | htpG |
cagtcagaagTGAAACAGCTTCTGCacctgatgat
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495157 | 495171 | [AIBSCS], [BPP] | [2] | |
| AscG | repressor | prpRp | Sigma70 | -58.5 | -87.0 | prpR |
tttcagttaaCGTTTCAggcaatgttt
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348527 | 348533 | [AIBSCS], [BPP], [GEA] | [2] | |
| AscG | repressor | prpRp | Sigma70 | -46.5 | -75.0 | prpR |
tttcaggcaaTGTTTCAccgcgtttca
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348515 | 348521 | [AIBSCS], [BPP], [GEA] | [2] | |
| AscG | repressor | prpRp | Sigma70 | -36.5 | -65.0 | prpR |
tgtttcaccgCGTTTCAttgcaacaat
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348505 | 348511 | [AIBSCS], [BPP], [GEA] | [2] | |
| AscG | repressor | prpRp | Sigma70 | -29.5 | -58.0 | prpR |
ccgcgtttcaTTGCAACaattatgaaa
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348498 | 348504 | [AIBSCS], [BPP], [GEA] | [2] | |
| AscG | repressor | prpRp | Sigma70 | -18.5 | -47.0 | prpR |
tgcaacaattATGAAACaagactaaac
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348487 | 348493 | [AIBSCS], [BPP], [GEA] | [2] | |
| AscG | repressor | ybiTp | nd | -108.5 | -124.0 | ybiT |
cagctcacagTGAAATCAGATGTGTacgaaatcac
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855832 | 855846 | [AIBSCS], [BPP] | [2] |
| Evolutionary conservation of regulatory elements | |
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| TF-target gene evolutionary conservation |
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| Promoter-target gene evolutionary conservation |
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| Reference(s) |
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[APPHINH] Assay of protein purified to homogeneity from its native host