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DicA DNA-binding transcriptional repressor

Synonyms: DicA
Summary:
The transcription factor DicA, "Division control," is a temperature-sensitive repressor that controls the transcription of genes involved in the cell division process [2, 4] and activation of its own expression [3]. Nevertheless, the signal to which DicA responds is still unknown. The family to which DicA belongs has not been established; it is a protein of 15 kDa with a hypothetical DNA-binding domain in the N-terminal domain []. DicA is homologous in structure and function to RovA (Yersinia) and SlyA (Salmonella), both of which are activators for pathogenicity-related genes [3].


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
DicA Functional   [APPHINH], [HIFS], [IEP], [IFC] [1], [2], [3]
Evolutionary Family: HTH_3
Connectivity class: Local Regulator
Gene name: dicA
  Genome position: 1647934-1648341
  Length: 408 bp / 135 aa
Operon name: dicA
TU(s) encoding the TF:
Transcription unit        Promoter
dicA
null


Regulon       
Regulated gene(s) dicB, dicC, insD-7, intQ, ydfD, ydfE, ydfW, ydfX
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
prophage genes and phage related functions (8)
cell cycle physiology (1)
Regulated operon(s) dicB-ydfDE-insD-7-intQ, dicC-ydfXW
First gene in the operon(s) dicB, dicC
Simple and complex regulons DicA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[DicA,-](1)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  DicA repressor dicBp Sigma70 nd -1649609.0 dicB, ydfD, ydfE, insD-7, intQ nd nd [GEA] [4]
  DicA repressor dicCp Sigma70 2.5 -25.0 dicC, ydfX, ydfW
aacatctatcTTGTTAGTTATGACTAACAAtaaaggtgtt
1647865 1647885 [BPP], [GEA], [IHBCE] [3]



High-throughput Transcription factor binding sites (TFBSs)
      

  Functional conformation Function Object name Object type Distance to first Gene Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  DicA activator dicA nd nd 1647865 1647885 [ICWHO] nd


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [APPHINH] Assay of protein purified to homogeneity from its native host

 [HIFS] Human inference of function from sequence

 [IEP] Inferred from expression pattern

 [IFC] Inferred by functional complementation

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins

 [IHBCE] Inferred by a human based on computational evidence

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Aldea M., Hernandez-Chico C., de la Campa AG., Kushner SR., Vicente M., 1988, Identification, cloning, and expression of bolA, an ftsZ-dependent morphogene of Escherichia coli., J Bacteriol 170(11):5169-76

 [2] Cam K., Bejar S., Gil D., Bouche JP., 1988, Identification and sequence of gene dicB: translation of the division inhibitor from an in-phase internal start., Nucleic Acids Res 16(14A):6327-38

 [3] Yun SH., Ji SC., Jeon HJ., Wang X., Kim SW., Bak G., Lee Y., Lim HM., 2012, The CnuK9E H-NS complex antagonizes DNA binding of DicA and leads to temperature-dependent filamentous growth in E. coli., PLoS One 7(9):e45236

 [4] Bejar S., Bouche JP., 1985, A new dispensable genetic locus of the terminus region involved in control of cell division in Escherichia coli., Mol Gen Genet 201(2):146-50



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