|Synonyms: ArsR-arsenite, ArsR-antimonite, ArsR|
ArsR negatively controls the expression of the genes involved in arsenical and antimonite metals resistance whose expression is induced in the presence of these metals |CITS:|. This protein is autoregulated; arsR is the first gene in the arsRBC operon that it regulates |CITS:|.
ArsR belongs to the ArsR/SmtB family of transcriptional regulators that respond to a variety of metals |CITS:|. ArsR has a helix-turn-helix motif for DNA binding, a metal-binding site |CITS:|, and a dimerization domain |CITS:|. The inducer-binding sites for the members of this family appear to have arisen by convergent evolution |CITS:|. In ArsR the inducer-binding site contains three cysteine residues that bind arsenite and antimonite specifically and with high affinity |CITS:|.
Dimerization of ArsR is required for DNA binding and its ability to act as a transcriptional repressor |CITS:|. The dimer recognizes and binds to a 12-2-12 inverted repeat |CITS:|, but the binding of arsenic or antimonite to ArsR causes a conformational change in it, leading to dissociation from DNA and hence derepression |CITS:|.
The chromosomal ArsR shows 75% identity to ArsR of Escherichia coli plasmid R773 and 26% identity to staphylococcal plasmid pI258 and pSX267 ArsR proteins |CITS:|.
ArsR when overexpressed has the capacity to remove arsenic species from contaminated water |CITS:[ 15294789] |.
ArsR: homologous to arsenate inducibility regulator |CITS:|.
Review: |CITS:|. Read more >
|Sensing class:||External sensing using transported metabolites|
|Connectivity class:||Local Regulator|
|Length:||354 bp / 117 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||arsB, arsC, arsR|
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
The Arsenite-Antimonite (Ars) Efflux Family (1)
Transcription related (1)
arsRRead more >
|First gene in the operon(s)||arsR|
|Simple and complex regulons|
|Simple and complex regulatory phrases||
Regulatory phrase (List of promoters regulated by the phrase)
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|ArsR||repressor||arsRp||Sigma70||-41.5||-57.5||arsR, arsB, arsC||
|3648459||3648482||[AIBSCS], [BPP], [GEA]||, , |
|Evolutionary conservation of regulatory elements|
 Busenlehner LS., Pennella MA., Giedroc DP., 2003, The SmtB/ArsR family of metalloregulatory transcriptional repressors: Structural insights into prokaryotic metal resistance., FEMS Microbiol Rev. 27(2-3):131-43