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SgrR DNA-binding transcriptional dual regulator

Synonyms: SgrR
Summary:
SugaR transport-related Regulator SgrR is negatively autoregulated and coordinately activates transcription of the divergent operon. This regulator coordinates the response to glucose-phosphate stress |CITS: [17209026][15522088][17383224]|. As the intracellular accumulation of glucose-6-phosphate correlates with SgrR induction, it has been proposed as its inducer |CITS: [17209026][15522088][17383224]|. Autoregulation of SgrR is not affected by phosphosugar stress |CITS: [17209026]|. In addition, the induction of sgrS expression by the nonmetabolizable glucose PTS substrate (+/-)-methyl glucoside is abolished in a sgrR mutant |CITS: [15522088]|.
The SgrR protein is a member of a novel family of regulators and contains an N terminal which is characteristic of winged-helix DNA-binding transcriptional regulators and a predicted C-terminal solute-binding domain |CITS: [17209026]|.


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
SgrR     nd nd
Evolutionary Family: MarR
Connectivity class: Local Regulator
Gene name: sgrR
  Genome position: 75644-77299
  Length: 1656 bp / 551 aa
Operon name: sgrR-sroA-tbpA-thiPQ
TU(s) encoding the TF:
Transcription unit        Promoter
sgrR-sroA-tbpA-thiPQ
sgrRp


Regulon       
Regulated gene(s) alaC, setA, sgrR, sgrS, sgrT, sroA, tbpA, thiP, thiQ
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
thiamin (3)
membrane (2)
alanine (1)
Electrochemical potential driven transporters (1)
other (mechanical, nutritional, oxidative stress) (1)
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Regulated operon(s) alaC, sgrR-sroA-tbpA-thiPQ, sgrST-setA
First gene in the operon(s) alaC, sgrR, sgrS
Simple and complex regulons Cra,SgrR
SgrR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[SgrR,+](2)
[SgrR,-](1)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  SgrR activator alaCp Sigma70 -167.5 -271.5 alaC
aaaccacaatTTTAAAAGGGGATTGATAGATAAATGGTGTATTAgcaatgcatc
2498550 2498583 [GEA], [HIBSCS], [SM] [1]
  SgrR repressor sgrRp Sigma70 25.5 -14.5 sgrR, sroA, tbpA, thiP, thiQ
gaaaataaaaTTGCTGTCTTTTGCACAGGAGTTCCCCTTTTATGccatctgctc
77297 77330 [BPP], [GEA], [HIBSCS], [SM] [1], [2], [3]
  SgrR activator sgrSp Sigma70 -53.5 -53.5 sgrS, sgrT, setA
gagcagatggCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAAttttattttc
77297 77330 [BPP], [GEA], [HIBSCS], [SM] [1], [2], [3]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences

 [SM] Site mutation

 [BPP] Binding of purified proteins



Reference(s)    

 [1] Vanderpool CK., Gottesman S., 2007, The novel transcription factor SgrR coordinates the response to glucose-phosphate stress., J Bacteriol. 189(6):2238-48

 [2] Sun Y., Vanderpool CK., 2011, Regulation and function of Escherichia coli sugar efflux transporter A (SetA) during glucose-phosphate stress., J Bacteriol. 193(1):143-53

 [3] Vanderpool CK., Gottesman S., 2004, Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system., Mol Microbiol. 54(4):1076-89



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