|Synonyms: ZntR-Zn2+, ZntR-Cd2+, ZntR-Hg, ZntR|
ZntR, zinc transport regulator, regulates a gene involved in the Zn(II), Cd(II), and Pb(II) transport systems |CITS:|.
In the presence of any of these ions, ZntR activates transcription of zntA by a DNA distortion mechanism that makes the promoter a better substrate for RNA polymerase |CITS:|. The interaction of ZntR with Zn(II) has been characterized |CITS:|, but its interaction with other ions has not been described. An inverted repeat sequence of 22 bp, located in the spacer between the -10 and the -35 boxes, has been identified as the ZntR dimer-binding site |CITS:, |.
The crystal structure of an N-terminally truncated fragment of ZntR bound to zinc was solved at a resolution of 19 Å |CITS:|. Each monomer of ZntR has three domains: a dimerization helix, a signal recognition site and a DNA-binding motif (helix-turn-helix) |CITS:|. Two zinc ions are bound to each monomer |CITS:|.
ZntR belongs to the MerR family, and although most members of this family are transcribed divergently from the genes they regulate, zntR is distant on the genome from its regulated gene |CITS:|.
The ClpXP and Lon proteases degrade ZntR, but the regulator is protected against proteolysis when it is bound to zinc and DNA. The ClpXP protease appears to need an adaptor to carry out proteolysis of ZntR |CITS:[ 17220226]|.
|Sensing class:||External sensing using transported metabolites|
|Connectivity class:||Local Regulator|
|Length:||426 bp / 141 aa|
|TU(s) encoding the TF:||
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
ATP proton motive force interconversion (1)
The P-type ATPase (P-ATPase) Superfamily (1)
|First gene in the operon(s)||zntA|
|Simple and complex regulons|
|Simple and complex regulatory phrases||
Regulatory phrase (List of promoters regulated by the phrase)
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|3606387||3606408||[BPP], [SM]||, |
|Evolutionary conservation of regulatory elements|
 Brocklehurst KR., Hobman JL., Lawley B., Blank L., Marshall SJ., Brown NL., Morby AP., 1999, ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli., Mol Microbiol. 31(3):893-902
 Outten CE., Outten FW., O'Halloran TV., 1999, DNA distortion mechanism for transcriptional activation by ZntR, a Zn(II)-responsive MerR homologue in Escherichia coli., J Biol Chem. 274(53):37517-24