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RcsB-BglJ DNA-binding transcriptional activator

Synonyms: RcsB-BglJ
Summary:
RcsB-BglJ is a pleiotropic transcriptional activator that coordinates regulation with global regulators, including CRP, LeuO, and HNS. BglJ-RcsB is active independently of RcsB phosphorylation [3, 4, 5] Regulation by heteromeric transcription factors is a rare event in the bacterial word [3] A 20-bp consensus sequence motif has been proposed for RcsB-BglJ based on analysis with the MEME suite [3]
RcsB-BglJ acts antagonistically as well as synergistically with additional transcription factors, such as HNS, CRP, and LeuO. Activation by RcsB-BglJ occurs both at non-HNS-repressed promoters as well as at promoters repressed by HNS and/or StpA [3]


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
RcsB-BglJ     nd nd
Evolutionary Family: LuxR/UhpA
Connectivity class: Local Regulator
Gene name: bglJ
  Genome position: 4604160-4604837
  Length: 678 bp / 225 aa
Operon name: yjjQ-bglJ
TU(s) encoding the TF:
Transcription unit        Promoter
yjjQ-bglJ
yjjQp
Gene name: rcsB
  Genome position: 2316177-2316827
  Length: 651 bp / 216 aa
Operon name: rcsDB
TU(s) encoding the TF:
Transcription unit        Promoter
rcsB
rcsBp1
rcsB
rcsBp2
rcsDB
rcsDp


Regulon       
Regulated gene(s) bglB, bglF, bglG, leuO, molR_1, sfsB, yecT, ygiZ, yidL, ykiA, ynbA, ynbB, ynbC, ynbD, ynjI, yqhG
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (4)
Transcription related (4)
activator (4)
operon (2)
posttranslational modification (1)
Read more >
Regulated operon(s) bglGFB, leuO, molR_1, sfsB, yecT, ygiZ, yidL, ykiA, ynbABCD, ynjI, yqhG
First gene in the operon(s) bglG, bglG, bglG, leuO, molR_1, sfsB, yecT, ygiZ, yidL, ykiA, ynbA, ynjI, yqhG
Simple and complex regulons CRP,Fis,H-NS,LeuO,RcsB-BglJ,StpA
CRP,RcsB-BglJ
H-NS,LeuO,RcsB-BglJ,StpA
LexA,RcsB-BglJ
RcsB-BglJ
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[RcsB-BglJ,+](12)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  RcsB-BglJ activator bglGp nd -95.5 -225.5 bglG, bglF, bglB
ttatatataaCTTTATAAATTCCTAAAATTacacaaagtt
3906783 3906802 [AIBSCS], [GEA], [HIBSCS], [SM] [1], [2], [3], [4]
  RcsB-BglJ activator bglGp2 nd -75.5 -225.5 bglG
ttatatataaCTTTATAAATTCCTAAAATTacacaaagtt
3906783 3906802 [AIBSCS], [GEA], [HIBSCS], [SM] [1], [2], [3], [4]
  RcsB-BglJ activator leuOp2 nd -58.5 -122.5 leuO
cagaacccgtATCTTTATGTTTTCCGAATTttactcattt
84236 84255 [GEA], [HIBSCS], [SM] [5]
  RcsB-BglJ activator molR_1p Sigma70 -66.5 -95.5 molR_1
agcgccttttCTTTATAAATTCCTAAAGTTgttttcttgc
2196369 2196388 [AIBSCS], [GEA] [3]
  RcsB-BglJ activator sfsBp nd -67.5 -118.5 sfsB
ggatgtgcatCTTTATATATTCTGAATATTcacactcttt
3334781 3334800 [AIBSCS], [GEA] [3]
  RcsB-BglJ activator yecTp nd -68.5 -105.5 yecT
ttattgccatCTAAATGTATTCTGAAAATGgacatgccat
1961857 1961876 [AIBSCS], [GEA] [3]
  RcsB-BglJ activator ygiZp nd -65.5 -177.5 ygiZ
ccgatgcaggATAAATATTTTCTGAAAATGacaatactga
3172379 3172398 [AIBSCS], [GEA] [3]
  RcsB-BglJ activator yidLp nd -51.5 -81.5 yidL
gtcaggcagtCAGATTCTTAAAATACAAACgtcgtatccc
3860192 3860211 [AIBSCS], [GEA] [3]
  RcsB-BglJ activator ykiAp nd -78.5 -113.5 ykiA
cttgaacgggAGGGCATTTTTCTGAAATATcctttcttta
408486 408505 [AIBSCS], [GEA] [3]
  RcsB-BglJ activator ynbAp nd -65.5 -89.5 ynbA, ynbB, ynbC, ynbD
tccccaatacCTTGATATATTCTGAATTTTtaatgaaacg
1477522 1477541 [AIBSCS], [GEA] [3]
  RcsB-BglJ activator ynjIp nd -65.5 -94.5 ynjI
tcgtgctattCGAGTCATATTCTGAAATATccagcggatc
1844956 1844975 [AIBSCS], [GEA] [3]
  RcsB-BglJ activator yqhGp nd -44.5 -149.5 yqhG
ccgctaccggACTTTTCAGAAATCATTTATtcccctcgcg
3157491 3157510 [AIBSCS], [GEA] [3]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences

 [SM] Site mutation



Reference(s)    

 [1] Giel M., Desnoyer M., Lopilato J., 1996, A mutation in a new gene, bglJ, activates the bgl operon in Escherichia coli K-12., Genetics. 143(2):627-35

 [2] Madhusudan S., Paukner A., Klingen Y., Schnetz K., 2005, Independent regulation of H-NS-mediated silencing of the bgl operon at two levels: upstream by BglJ and LeuO and downstream by DnaKJ., Microbiology. 151(Pt 10):3349-59

 [3] Salscheider SL., Jahn A., Schnetz K., 2014, Transcriptional regulation by BglJ-RcsB, a pleiotropic heteromeric activator in Escherichia coli., Nucleic Acids Res. 42(5):2999-3008

 [4] Venkatesh GR., Kembou Koungni FC., Paukner A., Stratmann T., Blissenbach B., Schnetz K., 2010, BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl operon by H-NS., J Bacteriol. 192(24):6456-64

 [5] Stratmann T., Pul ¿¿., Wurm R., Wagner R., Schnetz K., 2012, RcsB-BglJ activates the Escherichia coli leuO gene, encoding an H-NS antagonist and pleiotropic regulator of virulence determinants., Mol Microbiol. 83(6):1109-23



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