|Synonyms: CreB, CreB-Phosphorylated|
CreB Carbon source responsive response regulator, is a DNA-binding transcriptional dual regulator |CITS:| and belongs to the CreBC two-component system (TCS) |CITS:|, which controls genes involved in acetate |CITS:| and ribose |CITS:| metabolism, in the maltose regulon |CITS:[ 9000616]|, in the pentose phosphate pathway |CITS:|, and genes which repair DNA damage associated with the replication fork |CITS:|.
CreBC regulates the expression of cre regulon genes in response to a switch from complex to minimal medium |CITS:|. It is active when cells are fermenting glycolytic carbon sources. Induction of the cre regulon during pyruvate growth is entirely CreC dependent |CITS:|.
CreBC is considered a global regulator that is positioned at the heart of metabolic control |CITS:|.
CreB and CreC are part of the creABCD operon |CITS:|. CreB binds in vitro to a TTCACnnnnnnTTCAC sequence called the cre tag direct repeat, which stimulates the cre regulon |CITS:|.
CreBC (TCS) is homologous (60-70%) with BrlAB of Aeromonas spp. at the amino acid level |CITS:|.
CreC can act as the the phosphate donor for PhoB in PhoR mutants (|CITS:| and references therein) but this activity is masked in PhoR+ strains and the CreC/PhoB pathway is not responsive to phosphate levels |CITS: |.
A model of regulation by CreBC was proposed by Cariss et al. (2008) in which CreC responds to changes in the growth medium and/or signal and autophosphorylates; later, its phosphate is passed on to CreB, which binds to cre tag sequences. CreB functions as a transcriptional activator by recruiting RNA polymerase, whereas the binding of CreB to the cre tag represses transcription through promoter occlusion |CITS:|.
The antimicrobial peptide lactoferricin B, binds to CreB and inhibits its phosphorylation in vitro |CITS: |.
|Sensing class:||External-Two-component systems|
|Connectivity class:||Local Regulator|
|Length:||690 bp / 229 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||creD, malE, malF, malG, talA|
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
creD, malF, malG
carbon compounds (2)
ABC superfamily, membrane component (2)
ABC superfamily, periplasmic binding component (1)
motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc) (1)
non-oxidative branch, pentose pathway (1)
|Regulated operon(s)||creABCD, malEFG, talA-tktB|
|First gene in the operon(s)||creD, malE, talA|
|Simple and complex regulons|
|Simple and complex regulatory phrases||
Regulatory phrase (List of promoters regulated by the phrase)
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence
||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|4638099||4638116||[AIBSCS], [BPP], [GEA]||, |
|CreB-Phosphorylated||repressor||malEp||Sigma70||18.5||-27.5||malE, malF, malG||
|Evolutionary conservation of regulatory elements|
 Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A., 2005, Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli., J Biol Chem. 280(2):1448-56
 Cariss SJ., Tayler AE., Avison MB., 2008, Defining the growth conditions and promoter-proximal DNA sequences required for activation of gene expression by CreBC in Escherichia coli., J Bacteriol. 190(11):3930-9