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CreB DNA-binding transcriptional dual regulator

Synonyms: CreB, CreB-Phosphorylated
CreB Carbon source responsive response regulator, is a DNA-binding transcriptional dual regulator |CITS:[11350954][18375564]| and belongs to the CreBC two-component system (TCS) |CITS:[2228961][11350954][18375564]|, which controls genes involved in acetate |CITS:[7883769]| and ribose |CITS:[10542272]| metabolism, in the maltose regulon |CITS:[ 9000616]|, in the pentose phosphate pathway |CITS:[8572885]|, and genes which repair DNA damage associated with the replication fork |CITS:[10224240]|.
CreBC regulates the expression of cre regulon genes in response to a switch from complex to minimal medium |CITS:[11350954]|. It is active when cells are fermenting glycolytic carbon sources. Induction of the cre regulon during pyruvate growth is entirely CreC dependent |CITS:[18375564]|.
CreBC is considered a global regulator that is positioned at the heart of metabolic control |CITS:[11350954]|.
CreB and CreC are part of the creABCD operon |CITS:[18375564]|. CreB binds in vitro to a TTCACnnnnnnTTCAC sequence called the cre tag direct repeat, which stimulates the cre regulon |CITS:[18375564]|.
CreBC (TCS) is homologous (60-70%) with BrlAB of Aeromonas spp. at the amino acid level |CITS:[11350954]|.
CreC can act as the the phosphate donor for PhoB in PhoR mutants (|CITS:[11350954]| and references therein) but this activity is masked in PhoR+ strains and the CreC/PhoB pathway is not responsive to phosphate levels |CITS: [7021308]|.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
CreB Non-Functional   Apo [BPP], [IPI] [1]
CreB-Phosphorylated Functional Covalent Holo [BPP], [IPI] [1]
Evolutionary Family: OmpR
Sensing class: External-Two-component systems
Connectivity class: Local Regulator
Gene name: creB
  Genome position: 4636007-4636696
  Length: 690 bp / 229 aa
Operon name: creABCD
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) creD, malE, malF, malG, talA
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
membrane (3)
carbon compounds (2)
ABC superfamily, membrane component (2)
ABC superfamily, periplasmic binding component (1)
motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc) (1)
Regulated operon(s) creABCD, malEFG, talA-tktB
First gene in the operon(s) creD, malE, talA
Simple and complex regulons CRP,CreB,Fis,MalT
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  CreB-Phosphorylated activator creDp nd -52.5 -70.5 creD
4638099 4638116 [AIBSCS], [BPP], [GEA] [2], [3]
  CreB-Phosphorylated repressor malEp Sigma70 18.5 -27.5 malE, malF, malG
4246438 4246455 [AIBSCS], [GEA] [2]
  CreB-Phosphorylated activator talAp nd -211.5 -269.5 talA
2578388 2578405 [AIBSCS], [GEA] [2]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [BPP] Binding of purified proteins

 [IPI] Inferred from physical interaction

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [GEA] Gene expression analysis


 [1] Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A., 2005, Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli., J Biol Chem. 280(2):1448-56

 [2] Avison MB., Horton RE., Walsh TR., Bennett PM., 2001, Escherichia coli CreBC is a global regulator of gene expression that responds to growth in minimal media., J Biol Chem. 276(29):26955-61

 [3] Cariss SJ., Tayler AE., Avison MB., 2008, Defining the growth conditions and promoter-proximal DNA sequences required for activation of gene expression by CreBC in Escherichia coli., J Bacteriol. 190(11):3930-9