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CreB DNA-binding transcriptional dual regulator

Synonyms: CreB-Phosphorylated, CreB
CreB "Carbon source responsive response regulator," is a DNA-binding transcriptional dual regulator [2, 3]and belongs to the CreBC two-component system (TCS) [2, 3, 4] which controls genes involved in acetate [5]and ribose [6]metabolism, in the maltose regulon [7] in the pentose phosphate pathway [8] and genes which repair DNA damage associated with the replication fork [9] CreBC regulates the expression of cre regulon genes in response to a switch from complex to minimal medium [2] It is active when cells are fermenting glycolytic carbon sources.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
CreB Non-Functional   Apo [BPP] [1]
CreB-Phosphorylated Functional Covalent Holo [AIFS], [APPH], [BPP], [IEP], [IPI] [1], [2], [3]
Evolutionary Family: OmpR
Sensing class: External-Two-component systems
Connectivity class: Local Regulator
Gene name: creB
  Genome position: 4636007-4636696
  Length: 690 bp / 229 aa
Operon name: creABCD
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) creD, malE, malF, malG, talA
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
membrane (3)
carbon compounds (2)
ABC superfamily, membrane component (2)
ABC superfamily, periplasmic binding component (1)
motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc) (1)
Regulated operon(s) creABCD, malEFG, talA-tktB
First gene in the operon(s) creD, malE, talA
Simple and complex regulons CRP,CreB,Fis,MalT
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence
LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  CreB-Phosphorylated activator creDp nd -52.5 -70.0 creD
4638099 4638117 [AIBSCS], [BPP], [GEA] [2], [3]
  CreB-Phosphorylated repressor malEp Sigma70 18.5 -28.0 malE, malF, malG
4246438 4246456 [AIBSCS], [GEA] [2]
  CreB-Phosphorylated activator talAp nd -211.5 -269.0 talA
2578388 2578406 [AIBSCS], [GEA] [2]

High-throughput Transcription factor binding sites (TFBSs)

  Functional conformation Function Object name Object type Distance to first Gene Sequence LeftPos RightPos Growth Condition Evidence (Confirmed, Strong, Weak) References
  CreB-Phosphorylated activator recG nd nd nd nd nd [AIBSCS], [GEA] [2]
  CreB-Phosphorylated activator nd nd nd nd nd nd [AIBSCS], [GEA] [2]
  CreB-Phosphorylated activator ackA-pta nd nd nd nd nd [AIBSCS], [GEA] [2]
  CreB-Phosphorylated activator nd nd nd nd nd nd [GEA], [HIBSCS] [2]
  CreB-Phosphorylated activator cbrA nd nd nd nd nd [AIBSCS], [GEA] [2]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [BPP] Binding of purified proteins

 [AIFS] Automated inference of function from sequence

 [APPH] Assay of protein purified to homogeneity

 [IEP] Inferred from expression pattern

 [IPI] Inferred from physical interaction

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences


 [1] Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A., 2005, Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli., J Biol Chem 280(2):1448-56

 [2] Avison MB., Horton RE., Walsh TR., Bennett PM., 2001, Escherichia coli CreBC is a global regulator of gene expression that responds to growth in minimal media., J Biol Chem 276(29):26955-61

 [3] Cariss SJ., Tayler AE., Avison MB., 2008, Defining the growth conditions and promoter-proximal DNA sequences required for activation of gene expression by CreBC in Escherichia coli., J Bacteriol 190(11):3930-9

 [4] Amemura M, Makino K, Shinagawa H, Nakata A, 1990, Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2., J Bacteriol, 1990 Nov

 [5] Kakuda H., Hosono K., Shiroishi K., Ichihara S., 1994, Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli., J Biochem 116(4):916-22

 [6] Dunn CA, O'Handley SF, Frick DN, Bessman MJ, 1999, Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance., J Biol Chem, 1999 Nov 5

 [7] Richet E., 1996, On the role of the multiple regulatory elements involved in the activation of the Escherichia coli malEp promoter., J Mol Biol 264(5):852-62

 [8] Sprenger GA, 1995, Genetics of pentose-phosphate pathway enzymes of Escherichia coli K-12., Arch Microbiol, 1995 Nov

 [9] Saveson CJ., Lovett ST., 1999, Tandem repeat recombination induced by replication fork defects in Escherichia coli requires a novel factor, RadC., Genetics 152(1):5-13

 [10] Wanner BL, Latterell P, 1980, Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli., Genetics, 1980 Oct

 [11] Ho YH, Sung TC, Chen CS, 2012, Lactoferricin B inhibits the phosphorylation of the two-component system response regulators BasR and CreB., Mol Cell Proteomics, 2012 Apr