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Synonyms: MetJ-S-adenosylmethionine, MetJ |
Summary:
MetJ represses the expression of genes involved in biosynthesis and transport of methionine [4, 12]. MetJ belongs to the ribbon-helix-helix family of regulatory proteins. The typical motif of members of this family, the ribbon-helix-helix motif for DNA binding, has been observed in the crystalline structure of MetJ [13]. This motif is composed of a two-stranded antiparallel β-ribbon that lies along the DNA major groove, two outer α-helices that interact with adjacent proteins along the DNA, and two inner β-helices that form the subunit interface [13]. MetJ as a homodimer [13, 14] recognizes and binds to an 8-bp DNA sequence called the met-box, which varies around a perfect palindromic consensus sequence [15, 16, 17]. MetJ finds its target sites rapidly by facilitated diffusion [18]. Read more > |
Transcription factor | ![]() ![]() |
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TF conformation(s): |
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Evolutionary Family: | MetJ | ||||||||||||||||||
Sensing class: | Using internal synthesized signals | ||||||||||||||||||
Connectivity class: | Local Regulator | ||||||||||||||||||
Gene name: | metJ | ||||||||||||||||||
Genome position: | 4128078-4128395 | ||||||||||||||||||
Length: | 318 bp / 105 aa | ||||||||||||||||||
Operon name: | metJ | ||||||||||||||||||
TU(s) encoding the TF: |
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Regulon |
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Regulated gene(s) | ahpC, ahpF, folE, metA, metB, metC, metE, metF, metI, metK, metL, metN, metQ, metR, yeiB | ||||||
Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
methionine (5)
detoxification (2)
folic acid (1)
amino acids (1)
threonine catabolism (1)
Read more >
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Regulated operon(s) | ahpCF, folE-yeiB, metA, metBL, metC, metE, metF, metK, metNIQ, metR | ||||||
First gene in the operon(s) | ahpC, folE, metA, metB, metC, metE, metF, metK, metN, metR, metR | ||||||
Simple and complex regulons | CRP,MetJ HypT,MetJ HypT,MetJ,PhoP MetJ MetJ,MetR Read more > | ||||||
Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) |
Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Growth Conditions | Evidence (Confirmed, Strong, Weak) | References | |
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MetJ-S-adenosylmethionine | repressor | ahpCp | Sigma70 | 17.5 | -7.5 | ahpC, ahpF |
catcgaaaacACGGAGGAAGTATAGAtgtccttgat
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638930 | 638945 | nd | [AIBSCS] | [3] | |
MetJ-S-adenosylmethionine | repressor | ahpCp | Sigma70 | 27.5 | 2.5 | ahpC, ahpF |
acggaggaagTATAGATGTCCTTGATtaacaccaaa
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638940 | 638955 | nd | [AIBSCS] | [3] | |
MetJ-S-adenosylmethionine | repressor | folEp | Sigma70 | 25.5 | -171.5 | folE, yeiB |
tttcacttccGTATTTGCATAACGATgttttaacat
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2243816 | 2243831 | nd | [AIBSCS], [BPP], [GEA] | [4] | |
MetJ-S-adenosylmethionine | repressor | folEp | Sigma70 | 33.5 | -163.5 | folE, yeiB |
ccgtatttgcATAACGATGTTTTAACatctgctgat
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2243808 | 2243823 | nd | [AIBSCS], [BPP], [GEA] | [4] | |
MetJ-S-adenosylmethionine | repressor | folEp | Sigma70 | 41.5 | -155.5 | folE, yeiB |
gcataacgatGTTTTAACATCTGCTGatgaaaggca
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2243800 | 2243815 | nd | [AIBSCS], [BPP], [GEA] | [4] | |
MetJ-S-adenosylmethionine | repressor | folEp | Sigma70 | 49.5 | -147.5 | folE, yeiB |
atgttttaacATCTGCTGATGAAAGGcagcggcaat
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2243792 | 2243807 | nd | [AIBSCS], [BPP], [GEA] | [4] | |
MetJ-S-adenosylmethionine | repressor | metAp1 | Sigma32 | 4.5 | -43.5 | metA |
ggttatcttcAGCTATCTGGATGTCTaaacgtataa
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4214229 | 4214244 | nd | [AIBSCS], [BCE], [BPP], [GEA] | [3], [4] | |
MetJ-S-adenosylmethionine | repressor | metAp1 | Sigma32 | 20.5 | -27.5 | metA |
ctggatgtctAAACGTATAAGCGTATgtagtgaggt
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4214245 | 4214260 | nd | [AIBSCS], [BCE], [BPP], [GEA] | [3], [4] | |
MetJ-S-adenosylmethionine | repressor | metBp | Sigma70 | -56.5 | -92.5 | metB, metL |
tcaatctataCGCAAAGAAGTTTAGAtgtccagatg
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4128572 | 4128587 | nd | [AIBSCS], [GEA] | [3], [4], [5] | |
MetJ-S-adenosylmethionine | repressor | metBp | Sigma70 | -48.5 | -84.5 | metB, metL |
tacgcaaagaAGTTTAGATGTCCAGAtgtattgacg
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4128580 | 4128595 | nd | [AIBSCS], [GEA] | [3], [4], [5] | |
MetJ-S-adenosylmethionine | repressor | metBp | Sigma70 | -46.5 | -82.5 | metB, metL |
cgcaaagaagTTTAGATGTCCAGATGtattgacgtc
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4128582 | 4128597 | nd | [AIBSCS], [GEA] | [3], [4], [5] | |
MetJ-S-adenosylmethionine | repressor | metBp | Sigma70 | -40.5 | -76.5 | metB, metL |
gaagtttagaTGTCCAGATGTATTGAcgtccattaa
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4128588 | 4128603 | nd | [AIBSCS], [GEA] | [3], [4], [5] | |
MetJ-S-adenosylmethionine | repressor | metBp | Sigma70 | -38.5 | -74.5 | metB, metL |
agtttagatgTCCAGATGTATTGACGtccattaaca
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4128590 | 4128605 | nd | [AIBSCS], [GEA] | [3], [4], [5] | |
MetJ-S-adenosylmethionine | repressor | metBp | Sigma70 | -30.5 | -66.5 | metB, metL |
tgtccagatgTATTGACGTCCATTAAcacaatgttt
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4128598 | 4128613 | nd | [AIBSCS], [GEA] | [3], [4], [5] | |
MetJ-S-adenosylmethionine | repressor | metCp | Sigma70 | nd | nd | metC | nd | nd | nd | [BPP] | [6], [7] | ||
MetJ-S-adenosylmethionine | repressor | metEp | nd | 1.5 | -168.5 | metE |
aaccgccaatTATGGATGTGTAAACAtctggacggc
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4012877 | 4012892 |
[a] [b] |
[BPP] | [8] | |
MetJ-S-adenosylmethionine | repressor | metEp | nd | 116.5 | -53.5 | metE |
ggatgaataaACTTGCCGCCTTCCCTaaattcaaaa
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4012992 | 4013007 |
[a] [b] |
[BPP] | [8] | |
MetJ-S-adenosylmethionine | repressor | metFp | Sigma70 | -8.5 | -78.5 | metF |
gcccttcggcTTTTCCTTCATCTTTAcatctggacg
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4132530 | 4132545 | [b] | [BCE], [SM] | [9] | |
MetJ-S-adenosylmethionine | repressor | metFp | Sigma70 | 2.0 | -69.5 | metF |
cttttccttcATCTTTACATCTGGACgtctaaacgg
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4132539 | 4132554 | nd | [AIBSCS] | [3] | |
MetJ-S-adenosylmethionine | repressor | metFp | Sigma70 | 4.5 | -66.5 | metF |
ttccttcatcTTTACATCTGGACGTCtaaacggata
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4132542 | 4132557 | [b] | [BCE], [SM] | [9] | |
MetJ-S-adenosylmethionine | repressor | metFp | Sigma70 | 7.5 | -63.5 | metF |
cttcatctttACATCTGGACGTCTAAacggatagat
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4132545 | 4132560 | [b] | [AIBSCS] | [3] | |
MetJ-S-adenosylmethionine | repressor | metFp | Sigma70 | 9.5 | -61.5 | metF |
tcatctttacATCTGGACGTCTAAACggatagatgt
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4132547 | 4132562 | [b] | [AIBSCS] | [3] | |
MetJ-S-adenosylmethionine | repressor | metFp | Sigma70 | 17.5 | -53.5 | metF |
acatctggacGTCTAAACGGATAGATgtgcacaaca
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4132555 | 4132570 | [b] | [AIBSCS] | [3] | |
MetJ-S-adenosylmethionine | repressor | metFp | Sigma70 | 20.5 | -50.5 | metF |
tctggacgtcTAAACGGATAGATGTGcacaacacaa
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4132558 | 4132573 | [b] | [BCE], [SM] | [9] | |
MetJ-S-adenosylmethionine | repressor | metKp | Sigma70 | -14.5 | -143.5 | metK |
agaattgaccTAAAATAGCCATCCAGatgttaatcc
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3086555 | 3086570 | nd | [AIBSCS] | [3] | |
MetJ-S-adenosylmethionine | repressor | metKp | Sigma70 | -2.5 | -131.5 | metK |
aaatagccatCCAGATGTTAATCCATccataccgat
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3086567 | 3086582 | nd | [AIBSCS] | [3] | |
MetJ-S-adenosylmethionine | repressor | metNp | Sigma70 | -25.5 | -94.5 | metN, metI, metQ |
aacctgattgATTTAGACGTCTGGATgccttaacat
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222732 | 222747 | nd | [AIBSCS], [HIBSCS] | [3], [4], [10], [11] | |
MetJ-S-adenosylmethionine | repressor | metNp | Sigma70 | -17.5 | -86.5 | metN, metI, metQ |
tgatttagacGTCTGGATGCCTTAACatccatttca
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222724 | 222739 | nd | [AIBSCS], [GEA] | [3], [4], [10] | |
MetJ-S-adenosylmethionine | repressor | metRp1 | nd | -144.5 | -183.5 | metR |
ttttgaatttAGGGAAGGCGGCAAGTttattcatcc
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4012992 | 4013007 | [b] | [BPP] | [8] | |
MetJ-S-adenosylmethionine | repressor | metRp1 | nd | -29.5 | -68.5 | metR |
gccgtccagaTGTTTACACATCCATAattggcggtt
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4012877 | 4012892 | [b] | [BPP] | [8] | |
MetJ-S-adenosylmethionine | repressor | metRp2 | nd | -162.5 | -183.5 | metR |
ttttgaatttAGGGAAGGCGGCAAGTttattcatcc
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4012992 | 4013007 | [b] | [BPP] | [8] | |
MetJ-S-adenosylmethionine | repressor | metRp2 | nd | -47.5 | -68.5 | metR |
gccgtccagaTGTTTACACATCCATAattggcggtt
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4012877 | 4012892 | [b] | [BPP] | [8] |
Growth Condition |
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Alignment and PSSM for MetJ TFBSs | ![]() |
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Position weight matrix (PWM). |
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A 3 13 0 7 0 5 1 8 7 14 2 5 1 3 1 5 C 0 0 10 0 0 8 2 0 0 0 10 0 0 12 1 0 G 9 2 0 6 2 1 1 5 7 1 0 8 5 0 2 8 T 3 0 5 2 13 1 11 2 1 0 3 2 9 0 11 2 |
Evolutionary conservation of regulatory elements | ![]() |
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Reference(s) |
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