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HipAB DNA-binding transcriptional repressor

Synonyms: HipAB
Summary:
The transcriptional repressor HipB, for High persistence, is negatively autoregulated and controls the transcription of a critical persistence factor [3, 4, 5, 6, 7, 8]. hipB foms an operon with hipA, and the products of this operon are classified as a toxin (HipA)-antitoxin (HipB) system. This HipAB system is involved in the high persistence, which is the capacity of the bacteria to survive prolonged exposure to antibiotics [8, 9], and Kawano et al. also showed that this system is important for survival during the long-term stationary phase [3, 4, 5].
The crystal structure of the HipB-HipA-DNA complex has been solved at 2.68 Å resolution [10]. The complex is tetrameric and is comprised of a HipB homodimer that interacts with DNA, sandwiched by a monomer of HipA on each side [10].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
HipAB     nd nd
Evolutionary Family: HTH_3, HipA
Connectivity class: Local Regulator
Gene name: hipA
  Genome position: 1590854-1592176
  Length: 1323 bp / 440 aa
Operon name: hipBA
TU(s) encoding the TF:
Transcription unit        Promoter
hipBA
hipBp
Gene name: hipB
  Genome position: 1592176-1592442
  Length: 267 bp / 88 aa
Operon name: hipBA
TU(s) encoding the TF:
Transcription unit        Promoter
hipBA
hipBp


Regulon       
Regulated gene(s) hipA, hipB, mazE, mazF, relA
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
translation (2)
defense/survival (2)
starvation (2)
other (mechanical, nutritional, oxidative stress) (2)
cell killing (2)
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Regulated operon(s) hipBA, relA-mazEFG
First gene in the operon(s) hipB, relA
Simple and complex regulons HipAB,HipB
HipAB,HipB,IHF
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[HipAB,-](2)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  HipAB repressor hipBp Sigma70 -85.5 -125.5 hipB, hipA
atcctcctttTTATCCGCGATCGCGGATATcgcagcgttt
1592558 1592577 [BPP], [GEA], [HIBSCS] [1]
  HipAB repressor hipBp Sigma70 -57.5 -97.5 hipB, hipA
atcgcagcgtTTATCCCGTAGAGCGGATAAgatgtgtttc
1592530 1592549 [BPP], [GEA], [HIBSCS] [1]
  HipAB repressor hipBp Sigma70 -18.5 -58.5 hipB, hipA
ccagattgacTTATCCTCACTAAAGGATAAaacttataat
1592491 1592510 [BPP], [GEA], [HIBSCS] [1]
  HipAB repressor hipBp Sigma70 10.5 -30.5 hipB, hipA
aaaacttataATATCCCCTTAAGCGGATAAacttgctgtg
1592463 1592482 [BPP], [GEA], [HIBSCS] [1]
  HipAB repressor relAp1 Sigma70 5.0 -174.0 relA, mazE, mazF
atagtttatgTATCCTGTAACCCTGCAACGCTGGCTCGGGATAgcgaagcgtt
2913809 2913841 [BPP], [GEA] [2]


Alignment and PSSM for HipAB TFBSs    

Aligned TFBS of HipAB   
  Sequence
  AGTTTATCCGCTTAAGGGGATATTATAA
  GTTTTATCCTTTAGTGAGGATAAGTCAA
  CGTTTATCCCGTAGAGCGGATAAGATGT
  TTTTTATCCGCGATCGCGGATATCGCAG
  TATGTATCCTGTAACCCTGCAACGCTGG

Position weight matrix (PWM).   
A	1	1	0	0	0	5	0	0	0	0	0	0	4	2	2	0	1	0	0	4	1	5	2	0	2	0	3	2
C	1	0	0	0	0	0	0	5	5	1	2	0	0	0	2	1	3	0	0	1	0	0	1	1	1	2	0	0
G	1	2	0	1	0	0	0	0	0	2	2	1	0	2	0	4	1	4	5	0	0	0	0	3	1	0	2	2
T	2	2	5	4	5	0	5	0	0	2	1	4	1	1	1	0	0	1	0	0	4	0	2	1	1	3	0	1

PWM logo   


 


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences



Reference(s)    

 [1] Black DS., Irwin B., Moyed HS., 1994, Autoregulation of hip, an operon that affects lethality due to inhibition of peptidoglycan or DNA synthesis., J Bacteriol. 176(13):4081-91

 [2] Lin CY., Awano N., Masuda H., Park JH., Inouye M., 2013, Transcriptional Repressor HipB Regulates the Multiple Promoters in Escherichia coli., J Mol Microbiol Biotechnol. 23(6):440-447

 [3] Kawano H., Hirokawa Y., Mori H., 2009, Long-term survival of Escherichia coli lacking the HipBA toxin-antitoxin system during prolonged cultivation., Biosci Biotechnol Biochem. 73(1):117-23

 [4] Lewis K., 2005, Persister cells and the riddle of biofilm survival., Biochemistry (Mosc). 70(2):267-74

 [5] Keren I., Shah D., Spoering A., Kaldalu N., Lewis K., 2004, Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coli., J Bacteriol. 186(24):8172-80

 [6] Black DS., Kelly AJ., Mardis MJ., Moyed HS., 1991, Structure and organization of hip, an operon that affects lethality due to inhibition of peptidoglycan or DNA synthesis., J Bacteriol. 173(18):5732-9

 [7] Korch SB., Henderson TA., Hill TM., 2003, Characterization of the hipA7 allele of Escherichia coli and evidence that high persistence is governed by (p)ppGpp synthesis., Mol Microbiol. 50(4):1199-213

 [8] Balaban NQ., Merrin J., Chait R., Kowalik L., Leibler S., 2004, Bacterial persistence as a phenotypic switch., Science. 305(5690):1622-5

 [9] Inouye M., 2006, The discovery of mRNA interferases: implication in bacterial physiology and application to biotechnology., J Cell Physiol. 209(3):670-6

 [10] Schumacher MA., Piro KM., Xu W., Hansen S., Lewis K., Brennan RG., 2009, Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB., Science. 323(5912):396-401

 [11] Evdokimov A., Voznesensky I., Fennell K., Anderson M., Smith JF., Fisher DA., 2009, New kinase regulation mechanism found in HipBA: a bacterial persistence switch., Acta Crystallogr D Biol Crystallogr. 65(Pt 8):875-9

 [12] Schumacher MA., Balani P., Min J., Chinnam NB., Hansen S., Vulic M., Lewis K., Brennan RG., 2015, HipBA-promoter structures reveal the basis of heritable multidrug tolerance., Nature. 524(7563):59-64

 [13] Yamaguchi Y., Park JH., Inouye M., 2011, Toxin-antitoxin systems in bacteria and archaea., Annu Rev Genet. 45:61-79

 [14] Jayaraman R., 2008, Bacterial persistence: some new insights into an old phenomenon., J Biosci. 33(5):795-805



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