RegulonDB RegulonDB 9.2:Regulon Page

GadE-RcsB DNA-binding transcriptional activator

Synonyms: GadE-RcsB

Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
GadE-RcsB     nd nd
Evolutionary Family: LuxR/UhpA
Connectivity class: Local Regulator

Regulated gene(s) adiC, aslB, cadA, cadB, dctR, gadA, gadX, slp, yhiM
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
pH (4)
Porters (Uni-, Sym- and Antiporters) (2)
membrane (2)
chaperoning, repair (refolding) (1)
unknown (1)
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Regulated operon(s) adiC, aslB, cadBA, gadAXW, slp-dctR, yhiM
First gene in the operon(s) adiC, aslB, cadB, gadA, slp, yhiM
Simple and complex regulons AdiY,ArcA,CRP,FNR,Fis,GadE-RcsB,GadW,GadX,H-NS,RcsB,TorR
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Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  GadE-RcsB activator adiCp7 Sigma32 nd nd adiC nd nd [BPP] [1]
  GadE-RcsB activator aslBp Sigma54 nd nd aslB nd nd [BPP] [2]
  GadE-RcsB activator cadBp Sigma70 nd nd cadB, cadA nd nd [BPP], [GEA] [1], [3]
  GadE-RcsB activator gadAp Sigma38 -62.5 -89.5 gadA, gadX
3667660 3667679 [BPP], [GEA], [HIBSCS], [SM] [3], [4], [5], [6], [7], [8], [9], [10]
  GadE-RcsB activator slpp Sigma70 nd nd slp, dctR nd nd [BPP], [GEA] [1], [8]
  GadE-RcsB activator yhiMp Sigma38 nd nd yhiM nd nd [BPP] [2]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences

 [SM] Site mutation


 [1] Krin E., Danchin A., Soutourina O., 2010, RcsB plays a central role in H-NS-dependent regulation of motility and acid stress resistance in Escherichia coli., Res Microbiol. 161(5):363-371

 [2] Krin E., Danchin A., Soutourina O., 2010, Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli., BMC Microbiol. 10:273

 [3] Hommais F., Krin E., Coppee JY., Lacroix C., Yeramian E., Danchin A., Bertin P., 2004, GadE (YhiE): a novel activator involved in the response to acid environment in Escherichia coli., Microbiology. 150(Pt 1):61-72

 [4] Castanie-Cornet MP., Cam K., Bastiat B., Cros A., Bordes P., Gutierrez C., 2010, Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE., Nucleic Acids Res. 38(11):3546-54

 [5] Castanie-Cornet MP., Foster JW., 2001, Escherichia coli acid resistance: cAMP receptor protein and a 20 bp cis-acting sequence control pH and stationary phase expression of the gadA and gadBC glutamate decarboxylase genes., Microbiology. 147(Pt 3):709-15

 [6] Castanie-Cornet MP., Treffandier H., Francez-Charlot A., Gutierrez C., Cam K., 2007, The glutamate-dependent acid resistance system in Escherichia coli: essential and dual role of the His-Asp phosphorelay RcsCDB/AF., Microbiology. 153(Pt 1):238-46

 [7] Itou J., Eguchi Y., Utsumi R., 2009, Molecular mechanism of transcriptional cascade initiated by the EvgS/EvgA system in Escherichia coli K-12., Biosci Biotechnol Biochem. 73(4):870-8

 [8] Johnson MD., Burton NA., Gutierrez B., Painter K., Lund PA., 2011, RcsB Is Required for Inducible Acid Resistance in Escherichia coli and Acts at gadE-Dependent and -Independent Promoters., J Bacteriol. 193(14):3653-6

 [9] Ma Z., Gong S., Richard H., Tucker DL., Conway T., Foster JW., 2003, GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in Escherichia coli K-12., Mol Microbiol. 49(5):1309-20

 [10] Tramonti A., De Canio M., Delany I., Scarlato V., De Biase D., 2006, Mechanisms of transcription activation exerted by GadX and GadW at the gadA and gadBC gene promoters of the glutamate-based acid resistance system in Escherichia coli., J Bacteriol. 188(23):8118-27