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HypT DNA-binding transcriptional dual regulator

Synonyms: HypT-Met-oxidized
Summary:
Based on microarray analysis it was shown that QseD, Quorum-sensing Escherichia coli regulator D, downregulates the flagellum regulon, decreasing motility 20494990


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
HypT-Met-oxidized Functional   [APPH], [APPHINH], [IEP], [IMP] [1], [2]
Evolutionary Family: LysR
Connectivity class: Local Regulator
Gene name: hypT
  Genome position: 4557378-4558289
  Length: 912 bp / 303 aa
Operon name: hypT
TU(s) encoding the TF:
Transcription unit        Promoter
hypT
null


Regulon       
Regulated gene(s) cydA, cydB, cysH, fecA, fecB, fecC, fecD, fecE, metB, metI, metL, metN, metQ
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
membrane (5)
Fe aquisition (4)
ABC superfamily, membrane component (3)
electron acceptors (2)
ABC superfamily, periplasmic binding component (2)
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Regulated operon(s) cydAB, cysJIH, fecABCDE, metBL, metNIQ
First gene in the operon(s) cydA, cysH, fecA, metB, metN
Simple and complex regulons ArcA,FNR,H-NS,HypT
CRP,Fur,HypT,PdhR
HypT
HypT,MetJ
HypT,MetJ,PhoP
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  HypT-Met-oxidized activator cydAp1 Sigma70 nd -771458.0 cydA, cydB nd nd [BPP], [GEA] [2]
  HypT-Met-oxidized activator cysHp nd nd 2888312.0 cysH nd nd [GEA] [1], [2]
  HypT-Met-oxidized repressor fecAp Sigma19 nd 4516677.0 fecA, fecB, fecC, fecD, fecE nd nd [GEA] [1], [2]
  HypT-Met-oxidized activator metBp Sigma70 nd -4128672.0 metB, metL nd nd [GEA] [1], [2]
  HypT-Met-oxidized activator metNp Sigma70 nd 222645.0 metN, metI, metQ nd nd [BPP], [GEA] [1], [2]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [APPH] Assay of protein purified to homogeneity

 [APPHINH] Assay of protein purified to homogeneity from its native host

 [IEP] Inferred from expression pattern

 [IMP] Inferred from mutant phenotype

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis



Reference(s)    

 [1] Drazic A., Miura H., Peschek J., Le Y., Bach NC., Kriehuber T., Winter J., 2013, Methionine oxidation activates a transcription factor in response to oxidative stress., Proc Natl Acad Sci U S A 110(23):9493-8

 [2] Gebendorfer KM., Drazic A., Le Y., Gundlach J., Bepperling A., Kastenmuller A., Ganzinger KA., Braun N., Franzmann TM., Winter J., 2012, Identification of a Hypochlorite-specific Transcription Factor from Escherichia coli., J Biol Chem 287(9):6892-903



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