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MatA DNA-binding transcriptional dual regulator

Synonyms: MatA
Summary:
MatA is a LuxR-type transcription factor that is directly involved, as a positive regulator acting at transcriptional and posttranscriptional levels, in the expression of the mat operon in meningitis isolate strain IHE 3034 (T. A. Lehti, P. Bauchart, M. Kukkonen, U. Dobrindt, T. K. Korhonen, B. Westerlund-Wikstr%o;m, unpublished results) [1]. On the other hand, MatA controls the expression of the flhDC operon, with higher affinity to flhDp, repressing flagellum biosynthesis, motility, and taxis [1].
The opposite regulatory actions of MatA on mat and on flhDC promoters advance the adaptation of E. coli from a planktonic to an adhesive lifestyle [1]. MatA binds more weakly to pfliA and to pfliC, and their levels are equal to those observed for matB [1].
MatA exerts a dual regulatory function on the choice of planktonic/sessile lifestyle and further supports the presence of antagonistic control of attachment and motility.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
MatA     nd nd
Evolutionary Family: LuxR/UhpA
Connectivity class: Local Regulator
Gene name: ecpR
  Genome position: 310746-311336
  Length: 591 bp / 196 aa
Operon name: ecpR
TU(s) encoding the TF:
Transcription unit        Promoter
ecpR
matAp1


Regulon       
Regulated gene(s) ecpA, ecpR, flhC, flhD, fliA, fliC, fliZ, tcyJ
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc) (4)
flagella (4)
flagellum (3)
Transcription related (3)
activator (2)
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Regulated operon(s) ecpA, ecpR, flhDC, fliAZ-tcyJ, fliC
First gene in the operon(s) ecpA, ecpR, flhD, flhD, fliA, fliC
Simple and complex regulons CRP,FliZ,Fur,H-NS,HdfR,IHF,LrhA,MatA,OmpR,QseB,RcsAB,YjjQ
CsgD,FlhDC,H-NS,MatA,NsrR,SutR
GadE,H-NS,MatA
MatA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  MatA repressor flhDp Sigma70 nd nd flhD, flhC nd nd [BPP], [GEA] [1]
  MatA repressor flhDp2 Sigma54 nd nd flhD, flhC nd nd [BPP], [GEA] [1]
  MatA repressor fliAp1 Sigma70 nd nd fliA, fliZ, tcyJ nd nd [BPP] [1]
  MatA repressor fliCp Sigma28 nd nd fliC nd nd [BPP] [1]
  MatA activator matAp1 Sigma24 nd nd ecpR nd nd [BPP] [1]
  MatA activator matBp5 Sigma70 nd nd ecpA nd nd [BPP] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis



Reference(s)    

 [1] Lehti TA., Bauchart P., Dobrindt U., Korhonen TK., Westerlund-Wikstrom B., 2012, The fimbriae activator MatA switches off motility in Escherichia coli by repression of the flagellar master operon flhDC., Microbiology. 158(Pt 6):1444-55



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