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FliZ DNA-binding transcriptional repressor

Synonyms: FliZ
Summary:
The transcriptional repressor FliZ controls the transcription of genes involved in the regulation of curli expression and the motility system [1, 2]. During the post-exponential growth phase, this regulator is an abundant protein in the genome, with about 21,500 molecules per cell.
Transcriptome expression analysis showed a global antagonistic effect between FliZ and σS that resulted in physiological traits, including flagellum-mediated motility and curli fimbria-mediated adhesion [1, 2]. FliZ interferes with binding within σS-dependent promoters, can also discriminate vegetative promoters, and can recognize alternative σS promoter-like sequences [2].
The C-terminal domain of FliZ contains an α-helix that is similar to helix 3.0 of σS, and this structure promotes DNA binding [2]. In FliZ, this α-helix contains a positively charged amino acid (R108) that is involved in recognition of the extended -10 promoter.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
FliZ     nd nd
Connectivity class: Local Regulator
Gene name: fliZ
  Genome position: 2000473-2001024
  Length: 552 bp / 183 aa
Operon name: fliAZ-tcyJ
TU(s) encoding the TF:
Transcription unit        Promoter
fliAZ-tcyJ
fliAp1
fliAZ-tcyJ
fliAp2


Regulon       
Regulated gene(s) csgA, csgB, csgC, csgD, csgE, csgF, csgG, flhC, flhD, gadB, gadC, gadE, hdeA, hdeB, mdtE, mdtF, mlrA, osmY, pdeR, yhiD
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
Transcription related (6)
activator (4)
pH (4)
defense/survival (3)
repressor (3)
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Regulated operon(s) csgBAC, csgDEFG, flhDC, gadBC, gadE-mdtEF, hdeAB-yhiD, mlrA, osmY, pdeR
First gene in the operon(s) csgB, csgD, csgD, flhD, gadB, gadE, hdeA, mlrA, osmY, pdeR
Simple and complex regulons AdiY,CRP,Fis,FliZ,GadE,GadW,GadX,RcsB
BasR,CRP,CpxR,Cra,CsgD,FliZ,IHF,MlrA,MqsA,OmpR,RcdA,RcsAB,RstA
CRP,EvgA,FliZ,GadE,GadW,GadX,H-NS,YdeO
CRP,Fis,FliZ,IHF,Lrp
CRP,FliZ,Fur,H-NS,HdfR,IHF,LrhA,MatA,OmpR,QseB,RcsAB,YjjQ
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Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  FliZ repressor csgBp Sigma38 nd nd csgB, csgA, csgC nd nd [GEA] [1]
  FliZ repressor csgDp1 Sigma38 nd nd csgD, csgE, csgF, csgG nd nd [BPP], [GEA] [1], [2]
  FliZ repressor csgDp2 Sigma38 nd nd csgD, csgE, csgF, csgG nd nd [BPP], [GEA] [1], [2]
  FliZ repressor flhDp Sigma70 nd nd flhD, flhC nd nd [BPP], [GEA] [2]
  FliZ repressor gadBp Sigma38 nd nd gadB, gadC nd nd [BPP], [GEA] [1], [2]
  FliZ repressor gadEp Sigma38 nd nd gadE, mdtE, mdtF nd nd [BPP], [GEA], [SM] [2]
  FliZ repressor gmrp Sigma38 nd nd pdeR nd nd [BPP], [GEA], [SM] [1], [2]
  FliZ repressor hdeAp Sigma38 nd nd hdeA, hdeB, yhiD nd nd [BPP], [GEA] [2]
  FliZ repressor mlrAp Sigma70 nd nd mlrA nd nd [BPP], [GEA], [SM] [1], [2]
  FliZ repressor osmYp Sigma70 nd nd osmY nd nd [BPP], [GEA] [1], [2]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins

 [SM] Site mutation



Reference(s)    

 [1] Pesavento C., Becker G., Sommerfeldt N., Possling A., Tschowri N., Mehlis A., Hengge R., 2008, Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli., Genes Dev. 22(17):2434-46

 [2] Pesavento C., Hengge R., 2012, The global repressor FliZ antagonizes gene expression by ¿¿S-containing RNA polymerase due to overlapping DNA binding specificity., Nucleic Acids Res. 40(11):4783-93

 [3] Mytelka DS., Chamberlin MJ., 1996, Escherichia coli fliAZY operon., J Bacteriol. 178(1):24-34



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