YehT belongs to the LytTR response regulator (RR) of the two-component system that is involved in the stationary-phase control network |CITS:|.
An exhaustive search of the Escherichia coli genome found a single motif for the YehT transcriptional regulator, located upstream of the yjiY gene; therefore, it seems to be the only target regulated by the YehU/YehT system |CITS:|.
Overproduction of YehT is associated with moderate resistance to growth-inhibiting agents, such as crystal violet and deoxycholate |CITS:|.
YehT protects three repeats of the motif upstream of the yjiY gene, which have been designated M1 to M3 and were identified using the MEME tool. The YehT-binding site consists of two direct repeats of the sequence motif ACC[G/A]CT[C/T]A, and these are linked by a 13-bp spacer |CITS:|.
An adenine nucleotide in front of M1 and hence M2 seems to be part of the DNA-binding motif. Both the M3 motif and the intervening spacer could be stabilizers for the YehT:DNA complex |CITS:|.
The YehU/YehT two-component system is one of the most widespread in bacteria and is also found in several human and plant pathogens |CITS:|. It is activated in response to the presence of peptides or amino acids |CITS:|. YehU/YehT has a negative effect on expression of the yhjX gene of the YpdA/YpdB system, which suggests an interconnectivity between the two systems |CITS:|.
The YehU/YehT and YpdA/YpdB two-component systems are activated and expression of the corresponding target genes was found to occur at the same time as the onset of the stationary phase |CITS:|.
In vivo studies detected crosswise stimulus-dependent alterations in the expression of their respective target genes, and mutational analyses revealed that deletion of any of the components of the signal transduction cascade affects the function of the other.
On the other hand, in vivo protein-protein interaction assays suggest that both systems together form a single large signaling unit |CITS:|.Read more >
Activation of the YehU/YehT signaling cascade, which results in YjiY production, suppresses YpdA/YpdB-mediated yhjX induction, whereas activation of the YpdA/YpdB signaling cascade, as well as inducing the synthesis of YhjX, promotes expression of yjiY |CITS:|.
|Sensing class:||External-Two-component systems|
|Connectivity class:||Local Regulator|
|Length:||720 bp / 239 aa|
|TU(s) encoding the TF:||
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|4591415||4591443||[BPP], [GEA], [SM]|||
|Evolutionary conservation of regulatory elements|