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YehT DNA-binding transcriptional activator

Synonyms: YehT-Phosphorylated
Summary:
YehT belongs to the LytTR response regulator (RR) of the two-component system that is involved in the stationary-phase control network [1, 2]
An exhaustive search of the Escherichia coli genome found a single motif for the YehT transcriptional regulator, located upstream of the yjiY gene; therefore, it seems to be the only target regulated by the YehU/YehT system [1]
Overproduction of YehT is associated with moderate resistance to growth-inhibiting agents, such as crystal violet and deoxycholate [3]
YehT protects three repeats of the motif upstream of the yjiY gene, which have been designated M1 to M3 and were identified using the MEME tool. The YehT-binding site consists of two direct repeats of the sequence motif ACC[G/A]CT[C/T]A, and these are linked by a 13-bp spacer [1]
An adenine nucleotide in front of M1 and hence M2 seems to be part of the DNA-binding motif. Both the M3 motif and the intervening spacer could be stabilizers for the YehT:DNA complex [1]
The YehU/YehT two-component system is one of the most widespread in bacteria and is also found in several human and plant pathogens [1] It is activated in response to the presence of peptides or amino acids [1] YehU/YehT has a negative effect on expression of the yhjX gene of the YpdA/YpdB system, which suggests an interconnectivity between the two systems [4]
The YehU/YehT and YpdA/YpdB two-component systems are activated and expression of the corresponding target genes was found to occur at the same time as the onset of the stationary phase [4]
In vivo studies detected crosswise stimulus-dependent alterations in the expression of their respective target genes, and mutational analyses revealed that deletion of any of the components of the signal transduction cascade affects the function of the other.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
YehT-Phosphorylated     nd nd
Evolutionary Family: LytTR
Sensing class: External-Two-component systems
Connectivity class: Local Regulator
Gene name: yehT
  Genome position: 2212243-2212962
  Length: 720 bp / 239 aa
Operon name: yehUT
TU(s) encoding the TF:
Transcription unit        Promoter
yehUT
null


Regulon       
Regulated gene(s) yjiY
Regulated operon(s) yjiY
First gene in the operon(s) yjiY
Simple and complex regulons CRP,YehT
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[YehT,+](1)


Transcription factor binding sites (TFBSs) arrangements       

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  YehT-Phosphorylated activator yjiYp Sigma38 -62.0 -150.0 yjiY
cgcaaaactgACCGCTTACATCCCTAAAATAACCACTCAgttatttacc
4591415 4591443 [BPP], [GEA], [SM] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [SM] Site mutation



Reference(s)    

 [1] Kraxenberger T., Fried L., Behr S., Jung K., 2012, First insights into the unexplored two-component system YehU/YehT in Escherichia coli., J Bacteriol. 194(16):4272-84

 [2] Nikolskaya AN., Galperin MY., 2002, A novel type of conserved DNA-binding domain in the transcriptional regulators of the AlgR/AgrA/LytR family., Nucleic Acids Res. 30(11):2453-9

 [3] Hirakawa H., Nishino K., Hirata T., Yamaguchi A., 2003, Comprehensive studies of drug resistance mediated by overexpression of response regulators of two-component signal transduction systems in Escherichia coli., J Bacteriol. 185(6):1851-6

 [4] Behr S., Fried L., Jung K., 2014, Identification of a Novel Nutrient-Sensing Histidine Kinase/Response Regulator Network in Escherichia coli., J Bacteriol. 196(11):2023-9



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