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BtsR DNA-binding transcriptional dual regulator

Synonyms: BtsR, BtsR-Phosphorylated
Summary:
YehT has similarity to two-component system response regulator proteins [3]. Overproduction causes a drug resistance phenotype [3, 4].


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
BtsR Non-Functional   Apo nd nd
BtsR-Phosphorylated Functional   Holo [APPHINH], [IEP], [IPI], [1], [2]
Evolutionary Family: LytTR
Sensing class: External-Two-component systems
Connectivity class: Local Regulator
Gene name: btsR
  Genome position: 2212243-2212962
  Length: 720 bp / 239 aa
Operon name: btsSR
TU(s) encoding the TF:
Transcription unit        Promoter
 
 


Regulon       
Regulated gene(s) aceE, aceF, btsT, csgA, csgB, csgC, csgD, csgE, csgF, csgG, cspC, lpd, mdh, pdhR, yobF
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (4)
pyruvate dehydrogenase (3)
glyoxylate degradation (3)
fimbri, pili (3)
Transcription related (3)
Read more >
Regulated operon(s) btsT, csgBAC, csgDEFG, mdh, pdhR-aceEF-lpd, yobF-cspC
First gene in the operon(s) csgB, csgD, mdh, pdhR, btsT, yobF
Simple and complex regulons ArcA,BtsR,CRP,DpiA,FlhDC
BasR,BtsR,CRP,CpxR,Cra,CsgD,FliZ,H-NS,IHF,MlrA,MqsA,OmpR,RcdA,RcsAB,RstA,ppGpp
BtsR
BtsR,CRP
BtsR,CRP,Cra,FNR,PdhR
Read more >
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[BtsR,-](5)
[BtsR,+](1)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  BtsR-Phosphorylated repressor csgBp Sigma38, Sigma70, Sigma38 62.0 -31.0 csgB, csgA, csgC
aaatgatttaATTTCTTAAATGtacgaccagg
1103915 1103926 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 -25.0 -173.0 csgD, csgE, csgF, csgG
tttaacacttGATTTAAGATTTgtaatggcta
1103364 1103375 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor csgDp1 Sigma38, Sigma70 37.0 -112.0 csgD, csgE, csgF, csgG
tatattttacCCATTTAGGGCTgatttattac
1103303 1103314 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor mdhp1 Sigma70 -145.0 -190.0 mdh
ccacctgttgGAATGTTGCGCTaatgcataag
3384453 3384464 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor mdhp1 Sigma70 -73.0 -118.0 mdh
ttaaatgcatAAACGCTAAACTtgcgtgacta
3384381 3384392 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor mdhp1 Sigma70 -49.0 -94.0 mdh
cgtgactacaCATTCTTGAGATgtggtcattg
3384357 3384368 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor pdhRp Sigma70, Sigma38 -203.0 -261.0 pdhR, aceE, aceF, lpd
tgaattaaaaATCACAAAATTGgtaagtgaat
121826 121837 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor pdhRp Sigma70, Sigma38 -188.0 -246.0 pdhR, aceE, aceF, lpd
aaaattggtaAGTGAATCGGTTcaattcggat
121841 121852 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated activator yjiYp Sigma38 -62.0 -150.0 btsT
ccgcaaaactGACCGCTTACATCCCTAAAATAACCACTCAgttatttacc
4591415 4591444 , [BPP], [CV(SM)], [GEA], [SM] [1], [2]
  BtsR-Phosphorylated repressor yobFp1 Sigma70 -207.0 -409.0 yobF, cspC
tttttttgctCATTCATCAATTtttcttattt
1907995 1908006 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor yobFp1 Sigma70 -174.0 -376.0 yobF, cspC
aaatttacaaTCCCTTTGGGGAttgacttctc
1907962 1907973 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]
  BtsR-Phosphorylated repressor yobFp1 Sigma70 -138.0 -340.0 yobF, cspC
gggtaattaaTAGCCGTTAACTgactgtttta
1907926 1907937 , [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [GEA], [GS] [2]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [APPHINH] Assay of protein purified to homogeneity from its native host

 [IEP] Inferred from expression pattern

 [IPI] Inferred from physical interaction

 [ISA] Inferred from Sequence Alignment

 [AIBSPD] Automated inference based on sequence pattern discovery

 [CV(GEA)] cross validation(GEA)

 [CV(GEA/GS)] cross validation(GEA/GS)

 [GEA] Gene expression analysis

 [GS] genomic SELEX

 [BPP] Binding of purified proteins

 [CV(SM)] cross validation(SM)

 [SM] Site mutation



Reference(s)    

 [1] Kraxenberger T., Fried L., Behr S., Jung K., 2012, First insights into the unexplored two-component system YehU/YehT in Escherichia coli., J Bacteriol 194(16):4272-84

 [2] Ogasawara H., Ishizuka T., Yamaji K., Kato Y., Shimada T., Ishihama A., 2019, Regulatory Role of Pyruvate-Sensing BtsSR in Biofilm Formation by Escherichia coli K-12., FEMS Microbiol Lett

 [3] Hirakawa H., Nishino K., Hirata T., Yamaguchi A., 2003, Comprehensive studies of drug resistance mediated by overexpression of response regulators of two-component signal transduction systems in Escherichia coli., J Bacteriol 185(6):1851-6

 [4] Hirakawa H., Nishino K., Yamada J., Hirata T., Yamaguchi A., 2003, Beta-lactam resistance modulated by the overexpression of response regulators of two-component signal transduction systems in Escherichia coli., J Antimicrob Chemother 52(4):576-82



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