Based on analysis with the Genomic SELEX screening system, PgrR was identified as a repressor of the expression of genes of the initial enzymes for peptidoglycan (PG) peptide degradation as well as genes of the switch control between recycling and degradation of PG peptides, which are induced upon exposure to heat shock or membrane distortion |CITS:|.
PgrR negatively controls ycjY-ymjD-ymjC-mpaA operon expression and binds to the palindromic sequence ATCATTT-AAATGAT |CITS:|. Both the pgrR and ycjY promoters are recognized by RpoH (σ32) and RpoE (σ24), both of which are involved in the heat shock response, and by FecI (σ19), which is involved in the response to extracytoplasmic stimuli |CITS:|.
Eleven possible targets were predicted to be under PgrR control. These targets were identified by Genomic SELEX analysis and include genes related to membrane formation and function: leuL, leuO, pheP, ycjY, pgrR, yedS, xapR, yfgF, yfgG, rfaL, and rfaS |CITS:|.
PgrR, Peptide glycan recycling Regulator.
A ycjZ mutant shows a defect in swarming motility, but no defect in swimming motility |CITS: |.
|Connectivity class:||Local Regulator|
|Length:||900 bp / 299 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||mpaA, tyrR, ycjF, ycjG, ycjX, ycjY, ymjC, ymjD|
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
Transcription related (1)
tyrRRead more >
|Regulated operon(s)||ycjG, ycjXF-tyrR, ycjY-ymjDC-mpaA|
|First gene in the operon(s)||tyrR, ycjG, ycjX, ycjY, ycjY|
|Simple and complex regulons|
|Simple and complex regulatory phrases||
Regulatory phrase (List of promoters regulated by the phrase)
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|PgrR||repressor||tyrRp||Sigma70||nd||nd||tyrR||nd||nd||[BPP], [GEA], [IGI]|||
|PgrR||repressor||ycjGp||nd||nd||nd||ycjG||nd||nd||[BPP], [GEA], [IGI]|||
|PgrR||repressor||ycjXp||Sigma32||nd||nd||ycjX, ycjF, tyrR||nd||nd||[BPP], [GEA], [IGI]|||
|PgrR||repressor||ycjYp6||Sigma32||113.5||-106.5||ycjY, ymjD, ymjC, mpaA||
|1391953||1391966||[BPP], [GEA], [IGI]||, |
|Evolutionary conservation of regulatory elements|
 Choi U., Park YH., Kim YR., Seok YJ., Lee CR., 2016, Increased expression of genes involved in uptake and degradation of murein tripeptide under nitrogen starvation in Escherichia coli., FEMS Microbiol Lett. 363(14)