RclR (formerly YkgD) is a redox-sensitive transcriptional activator that belongs to the AraC family, whose highly conserved cysteine residues are highly specifically sensitive to oxidation by reactive chlorine species (RCS)|CITS: |.
RclR relies on the reversible oxidation of conserved redox-sensitive cysteine residues to specifically sense RCS and control the expression of genes essential for survival under reactive chlorine (HOCl) stress |CITS: |.
RclR is activated by N-chlorotaurine, and that activation may involve formation of one disulfide bond. RclR contains two conserved cysteine residues (Cys-21 and Cys-89) and two conserved histidine residues (His-42 and His-75). Cys-21 and Cys-89 are important in the activation of RclR in vivo. Cys-21 could play the more critical role in the RclR redox response. Oxidation of both cysteines leads to strong, highly specific activation of expression of genes required for survival under RCS stress. The His residues are involved in preventing DNA binding when RclR levels are reduced |CITS: |.
RclR homologs are found in a variety of proteobacteria as well as in a few actinobacteria |CITS: |.
RclR: reactive chlorine resistance R |CITS: |
|Connectivity class:||Local Regulator|
|Length:||855 bp / 284 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||rclA, rclB, rclC|
|Multifun term(s) of regulated gene(s)||
rclA, rclB, rclC
|First gene in the operon(s)||rclA|
|Simple and complex regulons|
|Simple and complex regulatory phrases||Regulatory phrase (List of promoters regulated by the phrase)|
|Evolutionary conservation of regulatory elements|