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RclR DNA-binding transcriptional activator

Synonyms: RclR
Summary:
RclR (formerly YkgD) is a redox-sensitive transcriptional activator that belongs to the AraC family, whose highly conserved cysteine residues are highly specifically sensitive to oxidation by reactive chlorine species (RCS)[3]. RclR relies on the reversible oxidation of conserved redox-sensitive cysteine residues to specifically sense RCS and control the expression of genes essential for survival under reactive chlorine (HOCl) stress [3]. RclR is activated by N-chlorotaurine, and that activation may involve formation of one disulfide bond. RclR contains two conserved cysteine residues (Cys-21 and Cys-89) and two conserved histidine residues (His-42 and His-75). Cys-21 and Cys-89 are important in the activation of RclR in vivo. Cys-21 could play the more critical role in the RclR redox response. Oxidation of both cysteines leads to strong, highly specific activation of expression of genes required for survival under RCS stress. The His residues are involved in preventing DNA binding when RclR levels are reduced [3]. RclR homologs are found in a variety of proteobacteria as well as in a few actinobacteria [3]. RclR: "reactive chlorine resistance R" [3] Read more >


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
RclR Functional   [HIFS] [1], [2]
Evolutionary Family: AraC/XylS
Connectivity class: Local Regulator
Gene name: rclR
  Genome position: 320227-321081
  Length: 855 bp / 284 aa
Operon name: rclR
TU(s) encoding the TF:
Transcription unit        Promoter
 
 


Regulon       
Regulated gene(s) rclA, rclB, rclC
Multifun term(s) of regulated gene(s)
protection (3)
Regulated operon(s) rclABC
First gene in the operon(s) rclA
Simple and complex regulons RclR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[RclR,+](1)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  RclR activator ykgCp1 Sigma24 nd nd rclA, rclB, rclC
nd
 nd nd [BPP], [GEA] [3]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [HIFS] Human inference of function from sequence

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis


Reference(s)    

 [1] Perez-Rueda E., Collado-Vides J., 2000, The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12., Nucleic Acids Res 28(8):1838-47

 [2] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50

 [3] Parker BW., Schwessinger EA., Jakob U., Gray MJ., 2013, The RclR protein is a reactive chlorine-specific transcription factor in Escherichia coli., J Biol Chem 288(45):32574-84


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