SutR (formely YdcN) regulates a set of genes involved in the utilization of sulfur |CITS: |. SutR is a small transcription factor with a helix-turn-helix (HTH) motif and belongs to the Cro-C1-type superfamily |CITS: |. Based on genomic SELEX screen analysis, it was identified as a highly probable regulator for 12-16 operons involved in various steps of sulfur utilization, playing an important role in the coordination of expression of the genes involved in this metabolic pathway |CITS: |.
Genes identified by SELEX analysis include the following: paoC, xseB, thiI, ydcO, sutR, ynfG, ydiY, pfkB, fliZ, ypfN, yfgM, yfiC, tdcF, cysE, and yjcS. Four peaks were located in type A spacers of divergent transcription units (TUs), and eight peaks were identified within type B spacers upstream of one TU but downstream of the preceding TUs |CITS: |.
A direct repeat GCGT motif of the SutR box was found in the yfiC, ydcO, and sutR genes, which are associated with group A spacers, and the palindromic sequence of GCGT(Nn)ACGC was identified for yfiC, thiI, ydcO, and sutR genes |CITS: |. On the other hand, in the group B spacers, the direct repeat GCGT was identified upstream of the tdcF, yfgM, and ynfG genes while the palindromic GCGT(Nn)ACGC sequence was found in the fliZ gene |CITS: |.
Genes identified by microarray analysis include several for repression by SutR, sutR, ydcO, pfkB, fliZ, and cysE, while activation of SutR was identified only for ypfN |CITS: |.
The following genes were identified via chromatin immunoprecipitation (ChIP) in vivo: sutR, cysE, and fliZ |CITS: |.
The highest level of SutR binding was found in the spacer in the ydcO-sutR region.
Based on DNA footprinting assay results, two regions for SutR were identified in the ydcO-sutR spacer sequence. One of them that was identified, the strongest SutR-binding site, is called site 1, and it represses ydcO and activates sutR expression; it is a direct repeat, GCGT, separated by 4 bp. On the other hand, site 2 overlaps the -35 and -10 boxes of the sutRp promoter |CITS: |.Read more >
SutR, for regulator of sulfur utilization |CITS: |.
|Connectivity class:||Local Regulator|
|Length:||537 bp / 178 aa|
|TU(s) encoding the TF:||
|Regulated gene(s)||cysE, fliA, fliZ, sutR, tcyJ|
|Multifun term(s) of regulated gene(s)||
MultiFun Term (List of genes associated to the multifun term)
Transcription related (1)
sigma factors, anti-sigmafactors (1)
motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc) (1)
transcriptional level (1)
ABC superfamily, periplasmic binding component (1)
|Regulated operon(s)||cysE, fliAZ-tcyJ, sutR|
|First gene in the operon(s)||cysE, fliA, sutR|
|Simple and complex regulons|
|Simple and complex regulatory phrases||
Regulatory phrase (List of promoters regulated by the phrase)
|Functional conformation||Function||Promoter||Sigma factor||Central Rel-Pos||Distance to first Gene||Genes||Sequence||LeftPos||RightPos||Evidence (Confirmed, Strong, Weak)||References|
|SutR||repressor||fliAp1||Sigma70||nd||nd||fliA, fliZ, tcyJ||nd||nd||[AIBSCS], [BPP], [GEA]|||
|1506085||1506106||[BPP], [GEA], [HIBSCS]|||
|1506125||1506142||[BPP], [GEA], [HIBSCS]|||
|Evolutionary conservation of regulatory elements|