RegulonDB RegulonDB 11.1:Regulon Page

UvrY DNA-binding transcriptional activator

Synonyms: UvrY-phosphorylated, UvrY
The protein belongs to the LuxR/UhpA family. This regulator participates in controlling several genes involved in the DNA repair system and carbon metabolism. UvrY has been shown to be the cognate response regulator for the BarA sensor protein. UvrY phosphorylated at the conserved Asp54 residue is the active form [1]. Z nucleotides [ZMP (5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5-monophosphate) and/or ZTP (5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5-triphosphate)] can act as direct phosphodonors to UvrY in vivo, activating it in a BarA-independent manner [1]. UvrY requires the presence of IHF for binding to csrB but not csrC [2] The genes regulated by this two-component sytem have yet to be identified. The signal the system responds to is also not known, but may be associated with hydrogen peroxide-mediated stress [5]. However, it has been reported that UvrY is phosphorylated at the transition from the exponential to stationary phase of growth (OD600 1.3) [1]. Only two targets of UvrY were found in a Genomic SELEX screening; they are located upstream of the genes csrB and csrC [6]. Read more >

Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
UvrY Non-Functional   Apo nd nd nd
UvrY-phosphorylated Functional   Holo nd nd nd
Evolutionary Family: LuxR/UhpA
Sensing class: External-Two-component systems
Connectivity class: Local Regulator
Gene name: uvrY
  Genome position: 1994703-1995359
  Length: 657 bp / 218 aa
Operon name: uvrYC
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) csrB, csrC, luxS, uhpT, ydeP
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
RNA degradation (1)
posttranscriptional (1)
RNA related (1)
quorum sensing (1)
carbon compounds (1)
Read more >
Regulated operon(s) csrB, csrC, luxS, uhpT, ydeP
First gene in the operon(s) csrB, csrC, luxS, uhpT, ydeP
Simple and complex regulons CRP,UhpA,UvrY
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
  UvrY-phosphorylated activator csrBp Sigma70 -183.0 -183.0 csrB
  UvrY-phosphorylated activator csrCp nd -159.0 -159.0 csrC
  UvrY-phosphorylated activator luxSp2 nd nd nd luxS nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] S [3], [3]
  UvrY-phosphorylated activator uhpTp Sigma70 nd nd uhpT nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] S [4], [4]
  UvrY-phosphorylated activator ydePp Sigma70 nd nd ydeP nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] S [4], [4]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation