RegulonDB RegulonDB 10.8:Regulon Page

YhaJ DNA-binding transcriptional activator

Synonyms: YhaJ
YhaJ is a member of the LysR protein family. It was previously associated with regulation of virulence in an enterohemorrhagic Escherichia coli (EHEC) strain []. Its capability to bind to the promoter regions of several genes in E. coli and regulate their activity has been demonstrated [1].

Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
YhaJ Functional   [APPHINH], [HIFS], [IEP] [1], [2], [3]
Connectivity class: Local Regulator
Gene name: yhaJ
  Genome position: 3253318-3254214
  Length: 897 bp / 298 aa
Operon name: yhaJ
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) bssS
Multifun term(s) of regulated gene(s)
information transfer (1)
Regulated operon(s) bssS
First gene in the operon(s) bssS
Simple and complex regulons YhaJ
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  YhaJ activator bssSp1 Sigma32 nd nd bssS nd nd [BPP], [GEA] [1]

High-throughput Transcription factor binding sites (TFBSs)

  Functional conformation Function Object name Object type Distance to first Gene Sequence LeftPos RightPos Center Position Growth Condition Evidence (Confirmed, Strong, Weak) References
  YhaJ activator nd tu nd
nd nd nd nd [BPP], [GEA] [1]
  YhaJ activator ygiD tu nd
nd nd nd nd [BPP], [GEA] [1]
  YhaJ activator nd tu nd
nd nd nd nd [BPP], [GEA] [1]
  YhaJ activator nd tu nd
nd nd nd nd [BPP], [GEA] [1]
  YhaJ activator nd tu nd
nd nd nd nd [BPP], [GEA] [1]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [APPHINH] Assay of protein purified to homogeneity from its native host

 [HIFS] Human inference of function from sequence

 [IEP] Inferred from expression pattern

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis


 [1] Palevsky N., Shemer B., Connolly JP., Belkin S., 2016, The Highly Conserved Escherichia coli Transcription Factor YhaJ Regulates Aromatic Compound Degradation., Front Microbiol 7:1490

 [2] Perez-Rueda E., Collado-Vides J., 2000, The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12., Nucleic Acids Res 28(8):1838-47

 [3] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50