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HigB-HigA DNA-binding transcriptional repressor

Synonyms: HigB-HigA
Summary:
The crystal structure of the toxin-antitoxin (TA) HigBA complex has been resolved to 2.7 Å resolution. It displays a hetero-tetramer (HigBA)2 form comprised by two HigB and two HigA subunits [1] HigA and HigBA autoregulate differentially in vivo. Helix-turn-helix (HTH) folds of (HigBA)2 are responsible for their recognition of operator DNA [1]


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
HigB-HigA Functional   [APPHINH], [IHBCE] [1]
Evolutionary Family: SinR
Connectivity class: Local Regulator
Gene name: higA
  Genome position: 3233728-3234144
  Length: 417 bp / 138 aa
Operon name: higBA
TU(s) encoding the TF:
Transcription unit        Promoter
higBA
higBp
Gene name: higB
  Genome position: 3234141-3234455
  Length: 315 bp / 104 aa
Operon name: higBA
TU(s) encoding the TF:
Transcription unit        Promoter
higBA
higBp


Regulon       
Regulated gene(s) higA, higB
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
transcriptional level (1)
translation attenuation and efficiency (1)
Regulated operon(s) higBA
First gene in the operon(s) higB
Simple and complex regulons HigA,HigB-HigA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  HigB-HigA repressor higBp Sigma70 -27.0 -52.5 higB, higA
aatatgataaCATCCGTTGCCAGATTGGCAACGGATGaatttatctg
3234494 3234521 [BPP], [IHBCE] [1]
  HigB-HigA repressor higBp Sigma70 5.0 -21.5 higB, higA
cttcatcacgTCCGTTGACAAATTGGCAAtatgataaca
3234467 3234486 [BPP], [IHBCE] [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [APPHINH] Assay of protein purified to homogeneity from its native host

 [IHBCE] Inferred by a human based on computational evidence

 [BPP] Binding of purified proteins



Reference(s)    

 [1] Yang J., Zhou K., Liu P., Dong Y., Gao Z., Zhang J., Liu Q., 2016, Structural insight into the E. coli HigBA complex., Biochem Biophys Res Commun 478(4):1521-7



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