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BCCP DNA-binding transcriptional repressor

Synonyms: BCCP
Summary:
Biotin carboxyl carrier protein (BCCP) plays a central role in the acetyl-CoA carboxylase complex. The overall carboxylase reaction takes place in two distinct half-reactions. BCCP, which contains a biotinyl prosthetic group covalently attached to a specific lysyl residue, is carboxylated in the first partial reaction. In the second partial reaction the carboxyl group is transferred to an acceptor and BCCP is regenerated for further carboxylation 324999]|. On the other hand, BCCP amino terminal is able to bind to DNA; this means, BCCP autoregulates its coding gene expression (the accBD operon) [2]


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
BCCP Functional   nd [1], [2]
Connectivity class: Local Regulator


Regulon       
Regulated gene(s) accB, accC
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
biotin carboxyl carrier protein (1)
fatty acids and phosphatidic acid (1)
Regulated operon(s) accBC
First gene in the operon(s) accB
Simple and complex regulons BCCP
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  BCCP repressor accBp Sigma70 nd -3405436.0 accB, accC nd nd [GEA], [IMP] [2]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [GEA] Gene expression analysis

 [IMP] Inferred from mutant phenotype



Reference(s)    

 [1] Blanchard CZ., Chapman-Smith A., Wallace JC., Waldrop GL., 1999, The biotin domain peptide from the biotin carboxyl carrier protein of Escherichia coli acetyl-CoA carboxylase causes a marked increase in the catalytic efficiency of biotin carboxylase and carboxyltransferase relative to free biotin., J Biol Chem 274(45):31767-9

 [2] James ES., Cronan JE., 2004, Expression of two Escherichia coli acetyl-CoA carboxylase subunits is autoregulated., J Biol Chem 279(4):2520-7



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