Based on SELEX screening of Escherichia coli K-12 W3110, XynR, formerly known as YgadI, was identified as a regulator of xylonate catabolism; it is a rare single-target TF, and its regulation network is still fixed within the CR4-6 prophage without significant cross-talk with the host. It regulates the bidirectional transcripts yagA(b) and yagEF(GH) 
A single peak of XynR binding was identified within the spacer of bidirectional transcription units of the yagA and yagEF operons. However, by decreasing the cutoff of detection, approximately 30 low-level peaks were detected, which are all located inside open reading frames (ORFs)  The level of XynR was detectable in the exponential growth phase, but it decreased to an undetectable level during growth in the stationary phase 
XynR: xylonate catabolism regulator 
A yagI mutant shows increased biofilm formation compared to wild type, but a lower increase in biofilm formation when the derepressed conjugative plasmid R1drd19 is present .
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