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AccB DNA-binding transcriptional repressor

Synonyms: AccB
The accB gene encodes the biotin carboxyl carrier protein (BCCP), a component of acetyl CoA carboxylase [3]. AccB is active as a dimer []. The kinetics of the biotinylation reaction have been determined, and the N terminus does not appear to have any role in the modification []. Biotinylation causes a large structural change in the C-terminal region of the protein []. Biotinylation results in loss of conformational flexibility of the biotin interaction region []; a "thumb" domain comprising amino acids 94-101 fastens the biotin moiety to the surface of the protein [] and this interaction results in increased protein stability [].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
AccB Functional   nd [1], [2]
Connectivity class: Local Regulator
Gene name: accB
  Genome position: 3405436-3405906
  Length: 471 bp / 156 aa
Operon name: accBC
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) accB, accC
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
biotin carboxyl carrier protein (1)
fatty acids and phosphatidic acid (1)
Regulated operon(s) accBC
First gene in the operon(s) accB
Simple and complex regulons AccB
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  AccB repressor accBp Sigma70 nd nd accB, accC nd nd [CV(GEA)], [GEA], [IMP] [2]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [IMP] Inferred from mutant phenotype


 [1] Blanchard CZ., Chapman-Smith A., Wallace JC., Waldrop GL., 1999, The biotin domain peptide from the biotin carboxyl carrier protein of Escherichia coli acetyl-CoA carboxylase causes a marked increase in the catalytic efficiency of biotin carboxylase and carboxyltransferase relative to free biotin., J Biol Chem 274(45):31767-9

 [2] James ES., Cronan JE., 2004, Expression of two Escherichia coli acetyl-CoA carboxylase subunits is autoregulated., J Biol Chem 279(4):2520-7

 [3] Alix JH., 1989, A rapid procedure for cloning genes from lambda libraries by complementation of E. coli defective mutants: application to the fabE region of the E. coli chromosome., DNA 8(10):779-89

 [4] Reche P., Li YL., Fuller C., Eichhorn K., Perham RN., 1998, Selectivity of post-translational modification in biotinylated proteins: the carboxy carrier protein of the acetyl-CoA carboxylase of Escherichia coli., Biochem J 329 ( Pt 3):589-96

 [5] Li SJ., Cronan JE., 1992, The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase., J Biol Chem 267(2):855-63

 [6] Karow M., Fayet O., Georgopoulos C., 1992, The lethal phenotype caused by null mutations in the Escherichia coli htrB gene is suppressed by mutations in the accBC operon, encoding two subunits of acetyl coenzyme A carboxylase., J Bacteriol 174(22):7407-18