RegulonDB RegulonDB 10.6.3: Regulon Form
   
   


H-NS,Lrp regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
H-NS      Tractordb tool
Connectivity class Global Regulator
Sensing Class TFs for DNA-bending
Synonym(s): BglY, Cur, DNA-binding protein HLP-II (HU, BH2, HD, NS), DNA-binding transcriptional dual regulator H-NS, Drc, DrdX, Drs, FimG, Hns, HnsA, Irk, MsyA, OsmZ, PilG, TopS, TopX, VirR
Gene name(s): hns
Functional conformation(s): H-NS
Coregulator(s): AcrR, AdiY, ArcA, ArgP, ArgR, BasR, CRP, CadC, CaiF, CdaR, CpxR, Cra, CsgD, CspA, CysB, DksA, DksA-ppGpp, DnaA, DpiA, EvgA, FNR, Fis, FlhDC, FliZ, Fur, GadE, GadW, GadX, GalR, GalS, GlaR, GutM, H-NS, HU, HdfR, HypT, IHF, LacI, LeuO, LrhA, Lrp, MarA, MatA, McbR, MlrA, MntR, MqsA, NagC, NanR, NarL, NarP, NhaR, NrdR, NsrR, OmpR, OxyR, PhoB, PhoP, QseB, RcdA, RcsB, Rob, RstA, RutR, SdiA, SlyA, SoxS, SrlR, StpA, SutR, TorR, UvrY, YdeO, YjjQ, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
Lrp Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Sensing external and internal signals
Synonym(s): AlsB, DNA-binding transcriptional dual regulator Lrp, Ihb, LivR, Lrs, Lss, LstR, Mbf, OppI, RblA
Gene name(s): lrp
Functional conformation(s): Lrp , Lrp-leucine
Coregulator(s): AcrR, Ada, AdiY, AidB, ArcA, ArgP, ArgR, CRP, CadC, CpxR, Cra, DksA, DksA-ppGpp, EnvY, FNR, Fis, FliZ, Fur, GadE, GadW, GadX, GcvA, GlaR, H-NS, HU, HdfR, IHF, LrhA, Lrp, MarA, Mlc, ModE, Nac, NarL, NhaR, NsrR, OmpR, PhoP, PurR, RcsB, Rob, SoxS, StpA, TorR, YeiL, YjjQ, ppGpp
Summary: TF Summary ...[more]

REGULATION EXERTED BY H-NS,Lrp Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
H-NS repressor Lrp activator    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
Lrp activator ilvIHp1 ilvI , ilvH 85341 85355 -252.0 agacatgaatGTCTGGTTTATTCTGcattttttat [BCE]
[BPP]
[GEA]
[HIBSCS]
[SM]
[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
Lrp activator ilvIHp1 ilvI , ilvH 85372 85386 -221.0 ttattgaatgTAGAATTTTATTCTGaatgtgtggg [BCE]
[BPP]
[GEA]
[HIBSCS]
[SM]
[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
Lrp activator ilvIHp1 ilvI , ilvH 85453 85467 -140.0 ttgtgggattCAGCCGATTTATTATcaatttaatc [BCE]
[BPP]
[GEA]
[HIBSCS]
[SM]
[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
Lrp activator ilvIHp1 ilvI , ilvH 85488 85502 -105.0 ctctgtaatgGAGGATTTTATCGTTtcttttcacc [BCE]
[BPP]
[GEA]
[HIBSCS]
[SM]
[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
Lrp activator ilvIHp1 ilvI , ilvH 85517 85531 -76.0 ttcacctttcCTCCTGTTTATTCTTattaccccgt [BCE]
[BPP]
[GEA]
[HIBSCS]
[SM]
[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
Lrp activator ilvIHp1 ilvI , ilvH 85537 85551 -56.0 ttcttattacCCCGTGTTTATGTCTctggctgcca [BCE]
[BPP]
[GEA]
[HIBSCS]
[SM]
[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
Lrp activator osmCp2 osmC 1556537 1556551 -62.0 aatattttcgCCGGATTTTATTCGGaatatcctgc [BPP]
[GEA]
[HIBSCS]
[SM]
[11]
H-NS repressor osmCp2 osmC         [GEA] [11]
H-NS repressor ilvIHp1 ilvI , ilvH         [GEA] [12]

Evidence: [BCE] Binding of cellular extracts
[BPP] Binding of purified proteins
[GEA] Gene expression analysis
[HIBSCS] Human inference based on similarity to consensus sequences
[SM] Site mutation
Reference(s): [1] Haughn GW., et al., 1985
[2] Jafri S., et al., 2002
[3] Marasco R., et al., 1994
[4] Platko JV., et al., 1993
[5] Platko JV., et al., 1990
[6] Ricca E., et al., 1989
[7] Wang Q., et al., 1993
[8] Wang Q., et al., 1993
[9] Wang Q., et al., 1993
[10] Willins DA., et al., 1992
[11] Bouvier J., et al., 1998
[12] Levinthal M., et al., 1994



Reference(s)    

 [1] Haughn GW., Squires CH., De Felice M., Largo CT., Calvo JM., 1985, Unusual organization of the ilvIH promoter of Escherichia coli., J Bacteriol 163(1):186-98

 [2] Jafri S., Chen S., Calvo JM., 2002, ilvIH operon expression in Escherichia coli requires Lrp binding to two distinct regions of DNA., J Bacteriol 184(19):5293-300

 [3] Marasco R., Varcamonti M., La Cara F., Ricca E., De Felice M., Sacco M., 1994, In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli., J Bacteriol 176(17):5197-201

 [4] Platko JV., Calvo JM., 1993, Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine., J Bacteriol 175(4):1110-7

 [5] Platko JV., Willins DA., Calvo JM., 1990, The ilvIH operon of Escherichia coli is positively regulated., J Bacteriol 172(8):4563-70

 [6] Ricca E., Aker DA., Calvo JM., 1989, A protein that binds to the regulatory region of the Escherichia coli ilvIH operon., J Bacteriol 171(3):1658-64

 [7] Wang Q., Calvo JM., 1993, Lrp, a global regulatory protein of Escherichia coli, binds co-operatively to multiple sites and activates transcription of ilvIH., J Mol Biol 229(2):306-18

 [8] Wang Q., Calvo JM., 1993, Lrp, a major regulatory protein in Escherichia coli, bends DNA and can organize the assembly of a higher-order nucleoprotein structure., EMBO J 12(6):2495-501

 [9] Wang Q., Sacco M., Ricca E., Lago CT., De Felice M., Calvo JM., 1993, Organization of Lrp-binding sites upstream of ilvIH in Salmonella typhimurium., Mol Microbiol 7(6):883-91

 [10] Willins DA., Calvo JM., 1992, In vitro transcription from the Escherichia coli ilvIH promoter., J Bacteriol 174(23):7648-55

 [11] Bouvier J., Gordia S., Kampmann G., Lange R., Hengge-Aronis R., Gutierrez C., 1998, Interplay between global regulators of Escherichia coli: effect of RpoS, Lrp and H-NS on transcription of the gene osmC., Mol Microbiol 28(5):971-80

 [12] Levinthal M., Lejeune P., Danchin A., 1994, The H-NS protein modulates the activation of the ilvIH operon of Escherichia coli K12 by Lrp, the leucine regulatory protein., Mol Gen Genet 242(6):736-43


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