RegulonDB RegulonDB 10.6.3: Regulon Form
   
   


CRP,MalI regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
CRP Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB
Gene name(s): crp
Functional conformation(s): CRP-cAMP , CRP
Coregulator(s): AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BtsR, CRP, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FucR, Fur, GadE, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsB, RhaR, RhaS, Rob, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YiaJ, YjjQ, ZraR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
MalI      Tractordb tool
Connectivity class Local Regulator
Synonym(s): DNA-binding transcriptional repressor MalI
Gene name(s): malI
Functional conformation(s): MalI
Coregulator(s): CRP, MalI
Summary: TF Summary ...[more]

REGULATION EXERTED BY CRP,MalI Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
CRP activator MalI repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
CRP-cAMP activator malXp malX , malY 1699201 1699222 -101.5 tactccctgaTTATGTGACAGATAAAACGTTTtaccttttat [AIBSCS]
[BPP]
[GEA]
[SM]
[1]
[2]
CRP-cAMP activator malXp malX , malY 1699261 1699282 -41.5 tatcgttgcgTAATGTGATTTATGCCTCACTAaaatttgata [AIBSCS]
[BCE]
[BPP]
[GEA]
[SM]
[3]
[1]
[4]
[5]
MalI repressor malXp malX , malY 1699288 1699305 -16.5 cactaaaattTGATAAAACGTTTTATCTtctcgcgcaa [BCE]
[GEA]
[SM]
[3]
[4]
[5]
CRP dual MalI repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
MalI repressor malIp malI 1699210 1699227 10.5 gataaataaaAGGTAAAACGTTTTATCTgtcacataat [BCE]
[GEA]
[SM]
[3]
[6]
[4]
[5]
CRP-cAMP activator malIp malI 1699261 1699282 -43.5 tatcaaatttTAGTGAGGCATAAATCACATTAcgcaacgata [AIBSCS]
[BCE]
[BPP]
[GEA]
[SM]
[3]
[1]
[4]
[5]
CRP-cAMP repressor malIp malI 1699201 1699222 17.5 ataaaaggtaAAACGTTTTATCTGTCACATAAtcagggagta [AIBSCS]
[BPP]
[GEA]
[SM]
[1]
[2]

Evidence: [AIBSCS] Automated inference based on similarity to consensus sequences
[BPP] Binding of purified proteins
[GEA] Gene expression analysis
[SM] Site mutation
[BCE] Binding of cellular extracts
Reference(s): [1] Lloyd GS., et al., 2008
[2] Zheng D., et al., 2004
[3] Lloyd GS., et al., 2010
[4] Reidl J., et al., 1991
[5] Reidl J., et al., 1989
[6] Otsuka J., et al., 1996



Reference(s)    

 [1] Lloyd GS., Hollands K., Godfrey RE., Busby SJ., 2008, Transcription initiation in the Escherichia coli K-12 malI-malX intergenic region and the role of the cyclic AMP receptor protein., FEMS Microbiol Lett 288(2):250-7

 [2] Zheng D., Constantinidou C., Hobman JL., Minchin SD., 2004, Identification of the CRP regulon using in vitro and in vivo transcriptional profiling., Nucleic Acids Res 32(19):5874-93

 [3] Lloyd GS., Godfrey RE., Busby SJ., 2010, Targets for the MalI repressor at the divergent Escherichia coli K-12 malX-malI promoters., FEMS Microbiol Lett 305(1):28-34

 [4] Reidl J., Boos W., 1991, The malX malY operon of Escherichia coli encodes a novel enzyme II of the phosphotransferase system recognizing glucose and maltose and an enzyme abolishing the endogenous induction of the maltose system., J Bacteriol 173(15):4862-76

 [5] Reidl J., Romisch K., Ehrmann M., Boos W., 1989, MalI, a novel protein involved in regulation of the maltose system of Escherichia coli, is highly homologous to the repressor proteins GalR, CytR, and LacI., J Bacteriol 171(9):4888-99

 [6] Otsuka J., Watanabe H., Mori KT., 1996, Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli., J Theor Biol 178(2):183-204


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