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MetR,PurR regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
MetR Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional dual regulator MetR
Gene name(s): metR
Functional conformation(s): MetR-L-homocysteine , MetR
Coregulator(s): FNR, Fur, MetJ, MetR, NsrR, OxyR, PurR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
PurR Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional repressor PurR
Gene name(s): purR
Functional conformation(s): PurR-Guanine , PurR-hypoxanthine , PurR
Coregulator(s): CRP, DnaA, FNR, Fis, Fur, GadE, GcvA, IHF, Lrp, MarA, MetR, Nac, PepA, PhoP, PurR, RbsR, RutR
Summary: TF Summary ...[more]

REGULATION EXERTED BY MetR,PurR Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
MetR dual PurR repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
PurR-hypoxanthine repressor glyAp glyA 2685626 2685641 -59.5 cgtagcctgaAGGTAATCGTTTGCGTaaattccttt [AIBSCS]
[BPP]
[GEA]
[HIBSCS]
[SM]
[1]
[2]
[3]
[4]
MetR-L-homocysteine activator glyAp glyA 2685688 2685702 -121.0 catcacaaacCTGAAAAGAAATCCGtttccggttg [BPP]
[GEA]
[HIBSCS]
[SM]
[2]
[3]
[5]
[6]
MetR-L-homocysteine dual glyAp glyA 2685710 2685724 -143.0 atgtgatgaaGTGAAAAATTTGCATcacaaacctg [BPP]
[GEA]
[HIBSCS]
[IMP]
[SM]
[2]
[3]
[5]
[6]
[7]
MetR-L-homocysteine dual glyAp glyA 2685710 2685724 -143.0 atgtgatgaaGTGAAAAATTTGCATcacaaacctg [BPP]
[GEA]
[HIBSCS]
[IMP]
[SM]
[2]
[3]
[5]
[6]
[7]

Evidence: [AIBSCS] Automated inference based on similarity to consensus sequences
[BPP] Binding of purified proteins
[GEA] Gene expression analysis
[HIBSCS] Human inference based on similarity to consensus sequences
[SM] Site mutation
[IMP] Inferred from mutant phenotype
Reference(s): [1] Cho BK., et al., 2011
[2] Lorenz E., et al., 1995
[3] Lorenz E., et al., 1996
[4] Steiert JG., et al., 1992
[5] Lorenz E., et al., 1996
[6] Lorenz E., et al., 1996
[7] Membrillo-Hernandez J., et al., 1998



Reference(s)    

 [1] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64

 [2] Lorenz E., Stauffer GV., 1995, Characterization of the MetR binding sites for the glyA gene of Escherichia coli., J Bacteriol 177(14):4113-20

 [3] Lorenz E., Stauffer GV., 1996, RNA polymerase, PurR and MetR interactions at the glyA promoter of Escherichia coli., Microbiology 142 ( Pt 7):1819-24

 [4] Steiert JG., Kubu C., Stauffer GV., 1992, The PurR binding site in the glyA promoter region of Escherichia coli., FEMS Microbiol Lett 78(2-3):299-304

 [5] Lorenz E., Stauffer GV., 1996, MetR-mediated repression of the glyA gene in Escherichia coli., FEMS Microbiol Lett 144(2-3):229-33

 [6] Lorenz E., Stauffer GV., 1996, Cooperative MetR binding in the Escherichia coli glyA control region., FEMS Microbiol Lett 137(2-3):147-52

 [7] Membrillo-Hernandez J., Coopamah MD., Channa A., Hughes MN., Poole RK., 1998, A novel mechanism for upregulation of the Escherichia coli K-12 hmp (flavohaemoglobin) gene by the 'NO releaser', S-nitrosoglutathione: nitrosation of homocysteine and modulation of MetR binding to the glyA-hmp intergenic region., Mol Microbiol 29(4):1101-12


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