RegulonDB RegulonDB 10.6.3: Regulon Form
   
   


MetJ,MetR,OxyR regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
OxyR Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional dual regulator OxyR, MomR, Mor, bifunctional regulatory protein sensor for oxidative stress
Gene name(s): oxyR
Functional conformation(s): OxyR
Coregulator(s): CRP, ExuR, FNR, Fis, Fur, H-NS, IHF, IscR, MarA, MetJ, MetR, MntR, NarL, NarP, NsrR, OxyR, Rob, SoxS, UxuR, Zur
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
MetR Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional dual regulator MetR
Gene name(s): metR
Functional conformation(s): MetR-L-homocysteine , MetR
Coregulator(s): FNR, Fur, MetJ, MetR, NsrR, OxyR, PurR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
MetJ Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): B3938, MetJ-S-adenosylmethionine, MetJ-SAM
Gene name(s): metJ
Functional conformation(s): MetJ , MetJ-adenine , MetJ-MTA , MetJ-adenosylmethionine
Coregulator(s): CRP, HypT, MetJ, MetR, OxyR, PhoP
Summary: TF Summary ...[more]

REGULATION EXERTED BY MetJ,MetR,OxyR Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
MetJ repressor MetR activator OxyR activator    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
MetR-L-homocysteine activator metEp metE 4012815 4012829 -62.0 tacttcgatcATGAAAGTCCTTCACttcggcatga [BPP]
[GEA]
[HIBSCS]
[1]
[2]
[3]
[4]
[5]
MetJ-adenosylmethionine repressor metEp metE 4012992 4013007 116.5 ggatgaataaACTTGCCGCCTTCCCTaaattcaaaa [BPP] [1]
MetJ-adenosylmethionine repressor metEp metE 4012877 4012892 1.5 aaccgccaatTATGGATGTGTAAACAtctggacggc [BPP] [1]
MetR-L-homocysteine activator metEp metE 4012836 4012850 -41.0 tcacttcggcATGAATAATTTGCGCttgaggaata [BPP]
[GEA]
[1]
[2]
[3]
[4]
OxyR activator metEp metE 4012834 4012850 -42.0 cttcacttcgGCATGAATAATTTGCGCttgaggaata [CEUMA]
[IHBCE]
[RE]
[6]
MetJ repressor MetR repressor OxyR activator    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
MetR-L-homocysteine repressor metRp1 metR 4012815 4012829 34.0 tcatgccgaaGTGAAGGACTTTCATgatcgaagta [BPP]
[GEA]
[HIBSCS]
[1]
[2]
[3]
[4]
[5]
MetJ-adenosylmethionine repressor metRp1 metR 4012992 4013007 -144.5 ttttgaatttAGGGAAGGCGGCAAGTttattcatcc [BPP] [1]
MetJ-adenosylmethionine repressor metRp1 metR 4012877 4012892 -29.5 gccgtccagaTGTTTACACATCCATAattggcggtt [BPP] [1]
MetR-L-homocysteine repressor metRp1 metR 4012836 4012850 13.0 tattcctcaaGCGCAAATTATTCATgccgaagtga [BPP]
[GEA]
[1]
[2]
[3]
[4]
OxyR activator metRp1 metR 4012852 4012868 -5.0 taattggcggTTACTGTATATTCCTCAagcgcaaatt [CEUMA]
[IHBCE]
[RE]
[6]

Evidence: [BPP] Binding of purified proteins
[GEA] Gene expression analysis
[HIBSCS] Human inference based on similarity to consensus sequences
[CEUMA] ChIP-exo evidence used in manual assertion
[IHBCE] Inferred by a human based on computational evidence
[RE] RNA-seq evidence
Reference(s): [1] Cai XY., et al., 1989
[2] Cai XY., et al., 1989
[3] Jafri S., et al., 1995
[4] Maxon ME., et al., 1989
[5] Weissbach H., et al., 1991
[6] Seo SW., et al., 2015



Reference(s)    

 [1] Cai XY., Maxon ME., Redfield B., Glass R., Brot N., Weissbach H., 1989, Methionine synthesis in Escherichia coli: effect of the MetR protein on metE and metH expression., Proc Natl Acad Sci U S A 86(12):4407-11

 [2] Cai XY., Redfield B., Maxon M., Weissbach H., Brot N., 1989, The effect of homocysteine on MetR regulation of metE, metR and metH expression in vitro., Biochem Biophys Res Commun 163(1):79-83

 [3] Jafri S., Urbanowski ML., Stauffer GV., 1995, A mutation in the rpoA gene encoding the alpha subunit of RNA polymerase that affects metE-metR transcription in Escherichia coli., J Bacteriol 177(3):524-9

 [4] Maxon ME., Redfield B., Cai XY., Shoeman R., Fujita K., Fisher W., Stauffer G., Weissbach H., Brot N., 1989, Regulation of methionine synthesis in Escherichia coli: effect of the MetR protein on the expression of the metE and metR genes., Proc Natl Acad Sci U S A 86(1):85-9

 [5] Weissbach H., Brot N., 1991, Regulation of methionine synthesis in Escherichia coli., Mol Microbiol 5(7):1593-7

 [6] Seo SW., Kim D., Szubin R., Palsson BO., 2015, Genome-wide Reconstruction of OxyR and SoxRS Transcriptional Regulatory Networks under Oxidative Stress in Escherichia coli K-12 MG1655., Cell Rep 12(8):1289-99


RegulonDB