RegulonDB RegulonDB 10.7: Regulon Form
   
   


CRP,Mlc regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
CRP Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB
Gene name(s): crp
Functional conformation(s): CRP , CRP-cAMP
Coregulator(s): AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YiaJ, YjjQ, ZraR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
Mlc Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Synonym(s): DNA-binding transcriptional repressor Mlc, DgsA
Gene name(s): mlc
Functional conformation(s): Mlc-EIIGlC , Mlc
Coregulator(s): ArcA, CRP, Cra, Fis, Lrp, Mlc, NagC, SoxS
Summary: TF Summary ...[more]

REGULATION EXERTED BY CRP,Mlc Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
CRP repressor Mlc repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
CRP-cAMP repressor mlcp1 mlc 1668651 1668673 -58.5 aaaagcccgaAAAATGTGCTGTTAATCACATGCctaagtaaaa [AIBSCS]
[APIORCISFBSCS]
[GEA]
[1]
[2]
Mlc repressor mlcp1 mlc 1668577 1668599 16.0 tagcctacagATTATTTCGGAGCGCGAAAATATagggagtatg [APIORCISFBSCS]
[BCE]
[GEA]
[3]
[4]
[1]
CRP activator Mlc repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
CRP-cAMP activator mlcp2 ynfK , mlc 1668651 1668673 -70.5 aaaagcccgaAAAATGTGCTGTTAATCACATGCctaagtaaaa [AIBSCS]
[APIORCISFBSCS]
[GEA]
[1]
[2]
Mlc repressor mlcp2 ynfK , mlc 1668577 1668599 4.0 tagcctacagATTATTTCGGAGCGCGAAAATATagggagtatg [APIORCISFBSCS]
[BCE]
[GEA]
[3]
[4]
[1]

Evidence: [AIBSCS] Automated inference based on similarity to consensus sequences
[APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
[GEA] Gene expression analysis
[BCE] Binding of cellular extracts
Reference(s): [1] Shin D., et al., 2001
[2] Tsai MJ., et al., 2018
[3] Decker K., et al., 1998
[4] Plumbridge J. 2001



Reference(s)    

 [1] Shin D., Lim S., Seok YJ., Ryu S., 2001, Heat shock RNA polymerase (E sigma(32)) is involved in the transcription of mlc and crucial for induction of the Mlc regulon by glucose in Escherichia coli., J Biol Chem 276(28):25871-5

 [2] Tsai MJ., Wang JR., Yang CD., Kao KC., Huang WL., Huang HY., Tseng CP., Huang HD., Ho SY., 2018, PredCRP: predicting and analysing the regulatory roles of CRP from its binding sites in Escherichia coli., Sci Rep 8(1):951

 [3] Decker K., Plumbridge J., Boos W., 1998, Negative transcriptional regulation of a positive regulator: the expression of malT, encoding the transcriptional activator of the maltose regulon of Escherichia coli, is negatively controlled by Mlc., Mol Microbiol 27(2):381-90

 [4] Plumbridge J., 2001, DNA binding sites for the Mlc and NagC proteins: regulation of nagE, encoding the N-acetylglucosamine-specific transporter in Escherichia coli., Nucleic Acids Res 29(2):506-14


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