RegulonDB RegulonDB 10.7: Regulon Form
   
   


CRP,Lrp,Mlc regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
CRP Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB
Gene name(s): crp
Functional conformation(s): CRP , CRP-cAMP
Coregulator(s): AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YiaJ, YjjQ, ZraR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
Mlc Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Synonym(s): DNA-binding transcriptional repressor Mlc, DgsA
Gene name(s): mlc
Functional conformation(s): Mlc-EIIGlC , Mlc
Coregulator(s): ArcA, CRP, Cra, Fis, Lrp, Mlc, NagC, SoxS
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
Lrp Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Sensing external and internal signals
Synonym(s): AlsB, DNA-binding transcriptional dual regulator Lrp, Ihb, LivR, Lrs, Lss, LstR, Mbf, OppI, RblA
Gene name(s): lrp
Functional conformation(s): Lrp , Lrp-leucine
Coregulator(s): AcrR, Ada, AdiY, AidB, ArcA, ArgP, ArgR, CRP, CadC, CpxR, Cra, DksA, DksA-ppGpp, EnvY, FNR, Fis, FliZ, Fur, GadE, GadE-RcsB, GadW, GadX, GcvA, GlaR, H-NS, HU, HdfR, IHF, LrhA, Lrp, MarA, Mlc, ModE, Nac, NarL, NhaR, NsrR, OmpR, PhoP, PurR, QseB, RcsB, Rob, SoxS, StpA, TorR, YeiL, YjjQ, ppGpp
Summary: TF Summary ...[more]

REGULATION EXERTED BY CRP,Lrp,Mlc Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
CRP activator Lrp activator Mlc repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
CRP-cAMP activator malTp malT 3552943 3552964 -70.5 aaagatttggAATTGTGACACAGTGCAAATTCagacacataa [APIORCISFBSCS]
[BPP]
[GEA]
[1]
[2]
[3]
[4]
[5]
[6]
Mlc repressor malTp malT 3553024 3553046 12.0 ccttataaccATTAATTACGAAGCGCAAAAAAAataatatttc [APIORCISFBSCS]
[BCE]
[GEA]
[2]
[7]
Lrp activator malTp malT         [GEA] [8]

Evidence: [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
[BPP] Binding of purified proteins
[GEA] Gene expression analysis
[BCE] Binding of cellular extracts
Reference(s): [1] Chapon C., et al., 1983
[2] Decker K., et al., 1998
[3] Dethiollaz S., et al., 1996
[4] Eichenberger P., et al., 1997
[5] Eichenberger P., et al., 1996
[6] Menendez M., et al., 1987
[7] Plumbridge J. 2001
[8] Tchetina E., et al., 1995



Reference(s)    

 [1] Chapon C., Kolb A., 1983, Action of CAP on the malT promoter in vitro., J Bacteriol 156(3):1135-43

 [2] Decker K., Plumbridge J., Boos W., 1998, Negative transcriptional regulation of a positive regulator: the expression of malT, encoding the transcriptional activator of the maltose regulon of Escherichia coli, is negatively controlled by Mlc., Mol Microbiol 27(2):381-90

 [3] Dethiollaz S., Eichenberger P., Geiselmann J., 1996, Influence of DNA geometry on transcriptional activation in Escherichia coli., EMBO J 15(19):5449-58

 [4] Eichenberger P., Dethiollaz S., Buc H., Geiselmann J., 1997, Structural kinetics of transcription activation at the malT promoter of Escherichia coli by UV laser footprinting., Proc Natl Acad Sci U S A 94(17):9022-7

 [5] Eichenberger P., Dethiollaz S., Fujita N., Ishihama A., Geiselmann J., 1996, Influence of the location of the cAMP receptor protein binding site on the geometry of a transcriptional activation complex in Escherichia coli., Biochemistry 35(48):15302-12

 [6] Menendez M., Kolb A., Buc H., 1987, A new target for CRP action at the malT promoter., EMBO J 6(13):4227-34

 [7] Plumbridge J., 2001, DNA binding sites for the Mlc and NagC proteins: regulation of nagE, encoding the N-acetylglucosamine-specific transporter in Escherichia coli., Nucleic Acids Res 29(2):506-14

 [8] Tchetina E., Newman EB., 1995, Identification of Lrp-regulated genes by inverse PCR and sequencing: regulation of two mal operons of Escherichia coli by leucine-responsive regulatory protein., J Bacteriol 177(10):2679-83


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