RegulonDB

TRANSCRIPTION FACTOR:

Name:

Nac	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Synonym(s):

DNA-binding transcriptional dual regulator Nac

Gene name(s):

nac

Functional conformation(s):

Nac

Coregulator(s):

AdiY, ArgP, ArgR, AsnC, CRP, CytR, FNR, Fur, GadE, HdfR, IHF, Lrp, Nac, NsrR, NtrC, PhoB, PurR, QseB

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Name:

PurR	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

DNA-binding transcriptional repressor PurR

Gene name(s):

purR

Functional conformation(s):

PurR , PurR-Guanine , PurR-hypoxanthine

Coregulator(s):

CRP, DnaA, FNR, Fis, Fur, GadE, GcvA, IHF, Lrp, MarA, MetR, Nac, PepA, PhoP, PurR, RbsR, RutR

Summary:

TF Summary ...[more]

REGULATION EXERTED BY Nac,PurR

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
Nac activator PurR repressor
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
Nac	activator	codBp	codB , codA	354819	354833	-58.0	gcactcattcATATAAAAAATATATttccccacga	[AIBSCS] [BPP] [CV(GEA)] [CV(GEA)] [CV(GEA/SM)] [CV(SM)] [GEA] [SM]	[1] [2]
PurR-hypoxanthine	repressor	codBp	codB , codA	354840	354855	-36.5	atatttccccACGAAAACGATTGCTTtttatcttca	[AIBSCS] [APIORCISFBSCS] [CV(GEA)] [GEA]	[3] [4] [5]

Evidence:	[AIBSCS] Automated inference based on similarity to consensus sequences [BPP] Binding of purified proteins [CV(GEA)] cross validation(GEA) [CV(GEA/SM)] cross validation(GEA/SM) [CV(SM)] cross validation(SM) [GEA] Gene expression analysis [SM] Site mutation [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
Reference(s):	[1] Muse WB., et al., 2003 [2] Zimmer DP., et al., 2000 [3] Cho BK., et al., 2011 [4] Danielsen S., et al., 1992 [5] Kilstrup M., et al., 1989

Reference(s)
[1] Muse WB., Rosario CJ., Bender RA., 2003, Nitrogen regulation of the codBA (cytosine deaminase) operon from Escherichia coli by the nitrogen assimilation control protein, NAC., J Bacteriol 185(9):2920-6
[2] Zimmer DP., Soupene E., Lee HL., Wendisch VF., Khodursky AB., Peter BJ., Bender RA., Kustu S., 2000, Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation., Proc Natl Acad Sci U S A 97(26):14674-9
[3] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64
[4] Danielsen S., Kilstrup M., Barilla K., Jochimsen B., Neuhard J., 1992, Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase., Mol Microbiol 6(10):1335-44
[5] Kilstrup M., Meng LM., Neuhard J., Nygaard P., 1989, Genetic evidence for a repressor of synthesis of cytosine deaminase and purine biosynthesis enzymes in Escherichia coli., J Bacteriol 171(4):2124-7