RegulonDB RegulonDB 10.8: Regulon Form
   
   


Fur,PurR regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
PurR Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional repressor PurR
Gene name(s): purR
Functional conformation(s): PurR , PurR-Guanine , PurR-hypoxanthine
Coregulator(s): CRP, DnaA, FNR, Fis, Fur, GadE, GcvA, IHF, Lrp, MarA, MetR, Nac, PepA, PhoP, PurR, RbsR, RutR
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
Fur Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class External sensing using transported metabolites
Synonym(s): DNA-binding transcriptional dual regulator Fur
Gene name(s): fur
Functional conformation(s): Fur-Mn2+ , Fur-Fe2+ , Fur
Coregulator(s): AcrR, AdiY, ArcA, ArgP, ArgR, CRP, CdaR, CpxR, Cra, CusR, EnvY, FNR, Fis, FliZ, Fur, GadE, GadX, H-NS, HdfR, HprR, HypT, IHF, IscR, LrhA, Lrp, MarA, MatA, MetR, MntR, ModE, MqsA, Nac, NagC, NarL, NfeR, NrdR, NsrR, NtrC, OmpR, OxyR, PdhR, PhoB, PurR, QseB, RcnR, RcsAB, Rob, RstA, RutR, SlyA, SoxR, SoxS, YjjQ, Zur, ppGpp
Summary: TF Summary ...[more]

REGULATION EXERTED BY Fur,PurR Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
Fur repressor PurR repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
PurR-hypoxanthine repressor pyrCp pyrC 1122659 1122674 -23.5 tttcgtgcaaAGGAAAACGTTTCCGCttatcctttg [APIORCISFBSCS]
[BPP]
[CV(GEA)]
[CV(GEA)]
[CV(GEA/SM)]
[CV(SM)]
[GEA]
[SM]
[1]
[2]
[3]
PurR-hypoxanthine repressor purRp purR 1737784 1737799 103.5 aggtgtgtaaAGGCAAACGTTTACCTtgcgattttg [APIORCISFBSCS]
[BCE]
[BPP]
[CV(GEA)]
[CV(GEA)]
[CV(GEA/SM)]
[CV(SM)]
[GEA]
[SM]
[4]
[5]
[6]
PurR-hypoxanthine repressor purRp purR 1737872 1737887 191.5 gtagcgaaacGAGCAAACGTTTCCACtacaactgtg [APIORCISFBSCS]
[BCE]
[BPP]
[CV(GEA)]
[CV(GEA)]
[CV(GEA/SM)]
[CV(SM)]
[GEA]
[SM]
[5]
[6]
Fur-Fe2+ repressor purRp purR 1737631 1737649 -49.0 cgtcatttatAATGATAAGTGTTTTTACCacttcccctt [AIBSCS] [7]
Fur-Fe2+ repressor pyrCp pyrC 1122720 1122744 -88.5 gaagtgaagcCAGGCGAAATTGTCATTCCATTTACtgattaatca [APIORCISFBSCS]
[BPP]
[CV(GEA)]
[CV(GEA)]
[GEA]
[8]

Evidence: [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
[BPP] Binding of purified proteins
[CV(GEA)] cross validation(GEA)
[CV(GEA/SM)] cross validation(GEA/SM)
[CV(SM)] cross validation(SM)
[GEA] Gene expression analysis
[SM] Site mutation
[BCE] Binding of cellular extracts
[AIBSCS] Automated inference based on similarity to consensus sequences
Reference(s): [1] Choi KY., et al., 1990
[2] Wilson HR., et al., 1987
[3] Wilson HR., et al., 1990
[4] Cho BK., et al., 2011
[5] Meng LM., et al., 1990
[6] Rolfes RJ., et al., 1990
[7] Stojiljkovic I., et al., 1994
[8] Chai S., et al., 2007



Reference(s)    

 [1] Choi KY., Zalkin H., 1990, Regulation of Escherichia coli pyrC by the purine regulon repressor protein., J Bacteriol 172(6):3201-7

 [2] Wilson HR., Chan PT., Turnbough CL., 1987, Nucleotide sequence and expression of the pyrC gene of Escherichia coli K-12., J Bacteriol 169(7):3051-8

 [3] Wilson HR., Turnbough CL., 1990, Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12., J Bacteriol 172(6):3208-13

 [4] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64

 [5] Meng LM., Kilstrup M., Nygaard P., 1990, Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli., Eur J Biochem 187(2):373-9

 [6] Rolfes RJ., Zalkin H., 1990, Autoregulation of Escherichia coli purR requires two control sites downstream of the promoter., J Bacteriol 172(10):5758-66

 [7] Stojiljkovic I., Baumler AJ., Hantke K., 1994, Fur regulon in gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay., J Mol Biol 236(2):531-45

 [8] Chai S., Song CK., Kim SK., Park JH., Wee S., 2007, Effect of fur on pyrC gene expression., J Microbiol 45(6):583-9


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