RegulonDB

TRANSCRIPTION FACTOR:

Name:

MetR	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

DNA-binding transcriptional dual regulator MetR

Gene name(s):

metR

Functional conformation(s):

MetR , MetR-L-homocysteine

Coregulator(s):

FNR, Fur, MetJ, MetR, NsrR, OxyR, PurR, ppGpp

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Info

Name:

PurR	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

DNA-binding transcriptional repressor PurR

Gene name(s):

purR

Functional conformation(s):

PurR , PurR-Guanine , PurR-hypoxanthine

Coregulator(s):

CRP, DnaA, FNR, Fis, Fur, GadE, GcvA, IHF, Lrp, MarA, MetR, Nac, PepA, PhoP, PurR, RbsR, RutR

Summary:

TF Summary ...[more]

REGULATION EXERTED BY MetR,PurR

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
MetR dual PurR repressor
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
PurR-hypoxanthine	repressor	glyAp	glyA	2685626	2685641	-59.5	cgtagcctgaAGGTAATCGTTTGCGTaaattccttt	[AIBSCS] [APIORCISFBSCS] [BPP] [CV(GEA)] [CV(GEA)] [CV(GEA/SM)] [CV(SM)] [GEA] [SM]	[1] [2] [3] [4]
MetR-L-homocysteine	activator	glyAp	glyA	2685688	2685702	-121.0	catcacaaacCTGAAAAGAAATCCGtttccggttg	[APIORCISFBSCS] [BPP] [CV(GEA)] [CV(GEA)] [CV(GEA/SM)] [CV(SM)] [GEA] [SM]	[2] [3] [5] [6]
MetR-L-homocysteine	repressor	glyAp	glyA	2685710	2685724	-143.0	atgtgatgaaGTGAAAAATTTGCATcacaaacctg	[APIORCISFBSCS] [BPP] [CV(GEA)] [CV(GEA)] [CV(GEA/SM)] [CV(SM)] [GEA] [IMP] [SM]	[2] [3] [5] [6] [7]
MetR-L-homocysteine	activator	glyAp	glyA	2685710	2685724	-143.0	atgtgatgaaGTGAAAAATTTGCATcacaaacctg	[APIORCISFBSCS] [BPP] [CV(GEA)] [CV(GEA)] [CV(GEA/SM)] [CV(SM)] [GEA] [IMP] [SM]	[2] [3] [5] [6] [7]

Evidence:

[AIBSCS] Automated inference based on similarity to consensus sequences
[APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
[BPP] Binding of purified proteins
[CV(GEA)] cross validation(GEA)
[CV(GEA/SM)] cross validation(GEA/SM)
[CV(SM)] cross validation(SM)
[GEA] Gene expression analysis
[SM] Site mutation
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Cho BK., et al., 2011
[2] Lorenz E., et al., 1995
[3] Lorenz E., et al., 1996
[4] Steiert JG., et al., 1992
[5] Lorenz E., et al., 1996
[6] Lorenz E., et al., 1996
[7] Membrillo-Hernandez J., et al., 1998

Reference(s)
[1] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64
[2] Lorenz E., Stauffer GV., 1995, Characterization of the MetR binding sites for the glyA gene of Escherichia coli., J Bacteriol 177(14):4113-20
[3] Lorenz E., Stauffer GV., 1996, RNA polymerase, PurR and MetR interactions at the glyA promoter of Escherichia coli., Microbiology 142 ( Pt 7):1819-24
[4] Steiert JG., Kubu C., Stauffer GV., 1992, The PurR binding site in the glyA promoter region of Escherichia coli., FEMS Microbiol Lett 78(2-3):299-304
[5] Lorenz E., Stauffer GV., 1996, MetR-mediated repression of the glyA gene in Escherichia coli., FEMS Microbiol Lett 144(2-3):229-33
[6] Lorenz E., Stauffer GV., 1996, Cooperative MetR binding in the Escherichia coli glyA control region., FEMS Microbiol Lett 137(2-3):147-52
[7] Membrillo-Hernandez J., Coopamah MD., Channa A., Hughes MN., Poole RK., 1998, A novel mechanism for upregulation of the Escherichia coli K-12 hmp (flavohaemoglobin) gene by the 'NO releaser', S-nitrosoglutathione: nitrosation of homocysteine and modulation of MetR binding to the glyA-hmp intergenic region., Mol Microbiol 29(4):1101-12