RegulonDB

TRANSCRIPTION FACTOR:

Name:

HypT	Tractordb tool

Connectivity class

Local Regulator

Synonym(s):

DNA-binding transcriptional dual regulator HypT, QseD, YjiE

Gene name(s):

hypT

Functional conformation(s):

HypT-Met-oxidized

Coregulator(s):

ArcA, AscG, CRP, FNR, Fur, H-NS, HypT, MetJ, PdhR, PhoP

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Name:

MetJ	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

DNA-binding transcriptional repressor MetJ

Gene name(s):

metJ

Functional conformation(s):

MetJ-S-adenosyl-L-methionine , MetJ-MTA , MetJ-adenine , MetJ

Coregulator(s):

CRP, HypT, MetJ, MetR, OxyR, PhoP

Summary:

TF Summary ...[more]

REGULATION EXERTED BY HypT,MetJ

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
HypT activator MetJ repressor
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
MetJ-S-adenosyl-L-methionine	repressor	metNp	metQ , metI , metN	222732	222747	-25.5	aacctgattgATTTAGACGTCTGGATgccttaacat		[1] [2] [3]
MetJ-S-adenosyl-L-methionine	repressor	metNp	metQ , metI , metN	222724	222739	-17.5	tgatttagacGTCTGGATGCCTTAACatccatttca	[GEA]	[1] [2]
HypT-Met-oxidized	activator	metNp	metQ , metI , metN

Evidence:	[GEA] Gene expression analysis
Reference(s):	[1] Gal J., et al., 2002 [2] Marincs F., et al., 2006 [3] Merlin C., et al., 2002

Reference(s)
[1] Gal J., Szvetnik A., Schnell R., Kalman M., 2002, The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster., J Bacteriol 184(17):4930-2
[2] Marincs F., Manfield IW., Stead JA., McDowall KJ., Stockley PG., 2006, Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor., Biochem J 396(2):227-34
[3] Merlin C., Gardiner G., Durand S., Masters M., 2002, The Escherichia coli metD locus encodes an ABC transporter which includes Abc (MetN), YaeE (MetI), and YaeC (MetQ)., J Bacteriol 184(19):5513-7
[4] Drazic A., Miura H., Peschek J., Le Y., Bach NC., Kriehuber T., Winter J., 2013, Methionine oxidation activates a transcription factor in response to oxidative stress., Proc Natl Acad Sci U S A 110(23):9493-8
[5] Gebendorfer KM., Drazic A., Le Y., Gundlach J., Bepperling A., Kastenmuller A., Ganzinger KA., Braun N., Franzmann TM., Winter J., 2012, Identification of a Hypochlorite-specific Transcription Factor from Escherichia coli., J Biol Chem 287(9):6892-903

Reference(s)