RegulonDB RegulonDB 10.10: Regulon Form
   
   


CRP,NagC regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
CRP Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB
Gene name(s): crp
Functional conformation(s): CRP-cyclic-AMP , CRP
Coregulator(s): AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FrlR, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PlaR, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YjjQ, ZraR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
NagC Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class External sensing using transported metabolites
Synonym(s): DNA-binding transcriptional dual regulator NagC, NagR, transcriptional repressor of nag (N-acetylglucosamine) operon
Gene name(s): nagC
Functional conformation(s): NagC , NagC-N-acetyl-D-glucosamine 6-phosphate
Coregulator(s): AraC, BasR, CRP, ChbR, Cra, DksA, DksA-ppGpp, EvgA, FNR, Fur, GadX, GalR, GalS, H-NS, IHF, LexA, Mlc, NagC, NanR, NarL, NarP, OmpR, PhoP, RcsB, UvrY, ppGpp
Summary: TF Summary ...[more]

REGULATION EXERTED BY CRP,NagC Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
CRP activator NagC repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
CRP-cyclic-AMP activator ptsHp5 ptsH , ptsI , crr 2533427 2533448 -60.5 ttaggtgcttTTTTGTGGCCTGCTTCAAACTTtcgcccctcc [GEA] [1]
NagC repressor chiPp chiZ , chiP , chiQ 708203 708225 -22.0 gaaagagattGATAATTCGCGTCGCGAAAAATAgtctgttcct [GEA] [2]
NagC repressor ptsHp5 ptsH , ptsI , crr 2533502 2533524 16.0 aaccagactaATTATTTTGATGCGCGAAATTAAtcgttacagg    
NagC repressor chiPp chiZ , chiP , chiQ 707980 708004 -244.0 cggcgtttttTCTGCTTTTCTTCGCGAATTAATTCcgcttcgcaa [GEA] [2]
CRP-cyclic-AMP activator chiPp chiZ , chiP , chiQ 708158 708174 -70.5 catcaaaataTGTGCGGTTGCTCATGTtcttacattc   [2]
CRP dual NagC repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
CRP-cyclic-AMP activator nagBp umpH , nagC , nagA , nagB 703802 703823 -104.5 gagtgtgtgaAAATTTAATTCGTATCGCAAATtaaacgcgtg   [3]
CRP-cyclic-AMP activator nagBp umpH , nagC , nagA , nagB 703768 703789 -70.5 aacgcgtgtcTTTTGTGAGTTTTGTCACCAAAtatcgttatt [GEA] [3]
[4]
[5]
NagC repressor nagBp umpH , nagC , nagA , nagB 703798 703820 -101.0 tgtgtgaaaaTTTAATTCGTATCGCAAATTAAAcgcgtgtctt [GEA] [6]
[7]
[3]
[4]
[5]
NagC repressor nagBp umpH , nagC , nagA , nagB 703704 703726 -7.0 aaccagaaaaCTTATTTTATCATTCAAAAAATCaggtcggatt [GEA] [6]
[7]
[3]
[4]
[5]
CRP-cyclic-AMP activator nagEp nagE 703802 703823 -27.5 cacgcgtttaATTTGCGATACGAATTAAATTTtcacacactc   [3]
CRP-cyclic-AMP activator nagEp nagE 703768 703789 -61.5 aataacgataTTTGGTGACAAAACTCACAAAAgacacgcgtt [GEA] [7]
[3]
[4]
[5]
NagC repressor nagEp nagE 703704 703726 -125.0 aatccgacctGATTTTTTGAATGATAAAATAAGttttctggtt [GEA] [6]
[7]
[4]
[5]
NagC repressor nagEp nagE 703798 703820 -31.0 aagacacgcgTTTAATTTGCGATACGAATTAAAttttcacaca [GEA] [6]
[7]
[4]
[5]
CRP-cyclic-AMP repressor nagEp nagE 703802 703823 -27.5 cacgcgtttaATTTGCGATACGAATTAAATTTtcacacactc   [3]
CRP-cyclic-AMP repressor nagBp umpH , nagC , nagA , nagB 703802 703823 -104.5 gagtgtgtgaAAATTTAATTCGTATCGCAAATtaaacgcgtg   [3]
CRP-cyclic-AMP repressor nagEp nagE 703768 703789 -61.5 aataacgataTTTGGTGACAAAACTCACAAAAgacacgcgtt [GEA] [7]
[3]
[4]
[5]
CRP-cyclic-AMP repressor nagBp umpH , nagC , nagA , nagB 703768 703789 -70.5 aacgcgtgtcTTTTGTGAGTTTTGTCACCAAAtatcgttatt [GEA] [3]
[4]
[5]

Evidence: [GEA] Gene expression analysis
Reference(s): [1] Tanaka Y., et al., 1999
[2] Plumbridge J., et al., 2014
[3] Plumbridge J., et al., 1991
[4] Plumbridge J., et al., 1993
[5] Plumbridge J., et al., 1998
[6] Fernandez M., et al., 2019
[7] Plumbridge J. 2001



Reference(s)    

 [1] Tanaka Y., Kimata K., Inada T., Tagami H., Aiba H., 1999, Negative regulation of the pts operon by Mlc: mechanism underlying glucose induction in Escherichia coli., Genes Cells 4(7):391-9

 [2] Plumbridge J., Bossi L., Oberto J., Wade JT., Figueroa-Bossi N., 2014, Interplay of transcriptional and small RNA-dependent control mechanisms regulates chitosugar uptake in Escherichia coli and Salmonella., Mol Microbiol 92(4):648-58

 [3] Plumbridge J., Kolb A., 1991, CAP and Nag repressor binding to the regulatory regions of the nagE-B and manX genes of Escherichia coli., J Mol Biol 217(4):661-79

 [4] Plumbridge J., Kolb A., 1993, DNA loop formation between Nag repressor molecules bound to its two operator sites is necessary for repression of the nag regulon of Escherichia coli in vivo., Mol Microbiol 10(5):973-81

 [5] Plumbridge J., Kolb A., 1998, DNA bending and expression of the divergent nagE-B operons., Nucleic Acids Res 26(5):1254-60

 [6] Fernandez M., Plumbridge J., 2019, Complex synergistic amino acid-nucleotide interactions contribute to the specificity of NagC operator recognition and induction., Microbiology 165(7):792-803

 [7] Plumbridge J., 2001, DNA binding sites for the Mlc and NagC proteins: regulation of nagE, encoding the N-acetylglucosamine-specific transporter in Escherichia coli., Nucleic Acids Res 29(2):506-14


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