RegulonDB

TRANSCRIPTION FACTOR:

Name:

CRP	Weight Matrix Tractordb tool

Connectivity class

Global Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB

Gene name(s):

crp

Functional conformation(s):

CRP-cyclic-AMP , CRP

Coregulator(s):

AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FrlR, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PlaR, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YjjQ, ZraR, ppGpp

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Info

Name:

NagC	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

External sensing using transported metabolites

Synonym(s):

DNA-binding transcriptional dual regulator NagC, NagR, transcriptional repressor of nag (N-acetylglucosamine) operon

Gene name(s):

nagC

Functional conformation(s):

NagC , NagC-N-acetyl-D-glucosamine 6-phosphate

Coregulator(s):

AraC, BasR, CRP, ChbR, Cra, DksA, DksA-ppGpp, EvgA, FNR, Fur, GadX, GalR, GalS, H-NS, IHF, LexA, Mlc, NagC, NanR, NarL, NarP, OmpR, PhoP, RcsB, UvrY, ppGpp

Summary:

TF Summary ...[more]

REGULATION EXERTED BY CRP,NagC

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
CRP activator NagC repressor
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
CRP-cyclic-AMP	activator	ptsHp5	ptsH , ptsI , crr	2533427	2533448	-60.5	ttaggtgcttTTTTGTGGCCTGCTTCAAACTTtcgcccctcc	[GEA]	[1]
NagC	repressor	chiPp	chiZ , chiP , chiQ	708203	708225	-22.0	gaaagagattGATAATTCGCGTCGCGAAAAATAgtctgttcct	[GEA]	[2]
NagC	repressor	ptsHp5	ptsH , ptsI , crr	2533502	2533524	16.0	aaccagactaATTATTTTGATGCGCGAAATTAAtcgttacagg
NagC	repressor	chiPp	chiZ , chiP , chiQ	707980	708004	-244.0	cggcgtttttTCTGCTTTTCTTCGCGAATTAATTCcgcttcgcaa	[GEA]	[2]
CRP-cyclic-AMP	activator	chiPp	chiZ , chiP , chiQ	708158	708174	-70.5	catcaaaataTGTGCGGTTGCTCATGTtcttacattc		[2]
CRP dual NagC repressor
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
CRP-cyclic-AMP	activator	nagBp	umpH , nagC , nagA , nagB	703802	703823	-104.5	gagtgtgtgaAAATTTAATTCGTATCGCAAATtaaacgcgtg		[3]
CRP-cyclic-AMP	activator	nagBp	umpH , nagC , nagA , nagB	703768	703789	-70.5	aacgcgtgtcTTTTGTGAGTTTTGTCACCAAAtatcgttatt	[GEA]	[3] [4] [5]
NagC	repressor	nagBp	umpH , nagC , nagA , nagB	703798	703820	-101.0	tgtgtgaaaaTTTAATTCGTATCGCAAATTAAAcgcgtgtctt	[GEA]	[6] [7] [3] [4] [5]
NagC	repressor	nagBp	umpH , nagC , nagA , nagB	703704	703726	-7.0	aaccagaaaaCTTATTTTATCATTCAAAAAATCaggtcggatt	[GEA]	[6] [7] [3] [4] [5]
CRP-cyclic-AMP	activator	nagEp	nagE	703802	703823	-27.5	cacgcgtttaATTTGCGATACGAATTAAATTTtcacacactc		[3]
CRP-cyclic-AMP	activator	nagEp	nagE	703768	703789	-61.5	aataacgataTTTGGTGACAAAACTCACAAAAgacacgcgtt	[GEA]	[7] [3] [4] [5]
NagC	repressor	nagEp	nagE	703704	703726	-125.0	aatccgacctGATTTTTTGAATGATAAAATAAGttttctggtt	[GEA]	[6] [7] [4] [5]
NagC	repressor	nagEp	nagE	703798	703820	-31.0	aagacacgcgTTTAATTTGCGATACGAATTAAAttttcacaca	[GEA]	[6] [7] [4] [5]
CRP-cyclic-AMP	repressor	nagEp	nagE	703802	703823	-27.5	cacgcgtttaATTTGCGATACGAATTAAATTTtcacacactc		[3]
CRP-cyclic-AMP	repressor	nagBp	umpH , nagC , nagA , nagB	703802	703823	-104.5	gagtgtgtgaAAATTTAATTCGTATCGCAAATtaaacgcgtg		[3]
CRP-cyclic-AMP	repressor	nagEp	nagE	703768	703789	-61.5	aataacgataTTTGGTGACAAAACTCACAAAAgacacgcgtt	[GEA]	[7] [3] [4] [5]
CRP-cyclic-AMP	repressor	nagBp	umpH , nagC , nagA , nagB	703768	703789	-70.5	aacgcgtgtcTTTTGTGAGTTTTGTCACCAAAtatcgttatt	[GEA]	[3] [4] [5]

Evidence:	[GEA] Gene expression analysis
Reference(s):	[1] Tanaka Y., et al., 1999 [2] Plumbridge J., et al., 2014 [3] Plumbridge J., et al., 1991 [4] Plumbridge J., et al., 1993 [5] Plumbridge J., et al., 1998 [6] Fernandez M., et al., 2019 [7] Plumbridge J. 2001

Reference(s)
[1] Tanaka Y., Kimata K., Inada T., Tagami H., Aiba H., 1999, Negative regulation of the pts operon by Mlc: mechanism underlying glucose induction in Escherichia coli., Genes Cells 4(7):391-9
[2] Plumbridge J., Bossi L., Oberto J., Wade JT., Figueroa-Bossi N., 2014, Interplay of transcriptional and small RNA-dependent control mechanisms regulates chitosugar uptake in Escherichia coli and Salmonella., Mol Microbiol 92(4):648-58
[3] Plumbridge J., Kolb A., 1991, CAP and Nag repressor binding to the regulatory regions of the nagE-B and manX genes of Escherichia coli., J Mol Biol 217(4):661-79
[4] Plumbridge J., Kolb A., 1993, DNA loop formation between Nag repressor molecules bound to its two operator sites is necessary for repression of the nag regulon of Escherichia coli in vivo., Mol Microbiol 10(5):973-81
[5] Plumbridge J., Kolb A., 1998, DNA bending and expression of the divergent nagE-B operons., Nucleic Acids Res 26(5):1254-60
[6] Fernandez M., Plumbridge J., 2019, Complex synergistic amino acid-nucleotide interactions contribute to the specificity of NagC operator recognition and induction., Microbiology 165(7):792-803
[7] Plumbridge J., 2001, DNA binding sites for the Mlc and NagC proteins: regulation of nagE, encoding the N-acetylglucosamine-specific transporter in Escherichia coli., Nucleic Acids Res 29(2):506-14