RegulonDB

TRANSCRIPTION FACTOR:

Name:

PurR	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

DNA-binding transcriptional repressor PurR

Gene name(s):

purR

Functional conformation(s):

PurR-hypoxanthine , PurR-Guanine , PurR

Coregulator(s):

CRP, DnaA, FNR, Fis, Fur, GadE, GcvA, IHF, Lrp, MarA, MetR, Nac, PepA, PhoP, PurR, RbsR, RutR

Summary:

TF Summary ...[more]

REGULATION EXERTED BY PurR

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
PurR repressor
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
PurR-hypoxanthine	repressor	purCp	purC	2597772	2597787	2.5	ctgatatgatACGCAAACGTGTGCGTctgcaggaaa	[GEA]	[1] [2]
PurR-hypoxanthine	repressor	purLp	purL	2695620	2695635	-38.5	ttttatttccACGCAAACGGTTTCGTcagcgcatca	[GEA]	[1] [2] [3]
PurR-hypoxanthine	repressor	hflDp	purB , hflD	1191787	1191802	892.5	tttgctgccgACGCAATCGGTTACCTtgatgcaatc	[GEA]	[1] [4]
PurR-hypoxanthine	repressor	cvpAp1	ubiX , purF , cvpA	2430821	2430836	-28.5	aggaaatcccTACGCAAACGTTTTCTttttctgtta	[GEA]	[1] [2] [3] [5] [6]
PurR-hypoxanthine	repressor	purEp	purK , purE	553172	553187	-37.5	tttcacagccACGCAACCGTTTTCCTtgctctcttt	[GEA]	[1] [2] [3] [7]
PurR-hypoxanthine	repressor	glnBp2	glnB	2687476	2687491	20.5	cacgagctggATGCAAACGATTTCAAggaatgaatt	[GEA]	[8]
PurR-hypoxanthine	repressor	glnBp1	glnB	2687476	2687491	-42.5	cacgagctggATGCAAACGATTTCAAggaatgaatt	[GEA]	[8]
PurR-hypoxanthine	repressor	prsp	prs	1262218	1262233	-48.5	aggttatcgcAAGAAAACGTTTTCGCgaggttgatg	[GEA]	[8]
PurR-hypoxanthine	repressor	speAp	speB , speA	3086030	3086045	11.5	tgttatgaaaAAGAAACCGGTTGCGCagttggagcg	[GEA]	[8]
PurR-hypoxanthine	repressor	purTp	purT	1930828	1930844	-21.5	ataaagacacACGCAAACGTTTTCGTTtatactgcgc	[GEA]	[9]

Evidence:	[GEA] Gene expression analysis
Reference(s):	[1] Cho BK., et al., 2011 [2] He B., et al., 1990 [3] Meng LM., et al., 1990 [4] He B., et al., 1992 [5] Rolfes RJ., et al., 1988 [6] Rolfes RJ., et al., 1988 [7] Watanabe W., et al., 1989 [8] He B., et al., 1993 [9] Belliveau NM., et al., 2018

Reference(s)
[1] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64
[2] He B., Shiau A., Choi KY., Zalkin H., Smith JM., 1990, Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction., J Bacteriol 172(8):4555-62
[3] Meng LM., Kilstrup M., Nygaard P., 1990, Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli., Eur J Biochem 187(2):373-9
[4] He B., Smith JM., Zalkin H., 1992, Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR., J Bacteriol 174(1):130-6
[5] Rolfes RJ., Zalkin H., 1988, Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene., J Biol Chem 263(36):19649-52
[6] Rolfes RJ., Zalkin H., 1988, Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. Cloning, nucleotide sequence, and interaction with the purF operator., J Biol Chem 263(36):19653-61
[7] Watanabe W., Sampei G., Aiba A., Mizobuchi K., 1989, Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis., J Bacteriol 171(1):198-204
[8] He B., Choi KY., Zalkin H., 1993, Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor., J Bacteriol 175(11):3598-606
[9] Belliveau NM., Barnes SL., Ireland WT., Jones DL., Sweredoski MJ., Moradian A., Hess S., Kinney JB., Phillips R., 2018, Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria., Proc Natl Acad Sci U S A 115(21):E4796-E4805

Reference(s)

[1] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64

[2] He B., Shiau A., Choi KY., Zalkin H., Smith JM., 1990, Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction., J Bacteriol 172(8):4555-62

[3] Meng LM., Kilstrup M., Nygaard P., 1990, Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli., Eur J Biochem 187(2):373-9

[4] He B., Smith JM., Zalkin H., 1992, Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR., J Bacteriol 174(1):130-6

[5] Rolfes RJ., Zalkin H., 1988, Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene., J Biol Chem 263(36):19649-52

[6] Rolfes RJ., Zalkin H., 1988, Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. Cloning, nucleotide sequence, and interaction with the purF operator., J Biol Chem 263(36):19653-61

[7] Watanabe W., Sampei G., Aiba A., Mizobuchi K., 1989, Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis., J Bacteriol 171(1):198-204

[8] He B., Choi KY., Zalkin H., 1993, Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor., J Bacteriol 175(11):3598-606

[9] Belliveau NM., Barnes SL., Ireland WT., Jones DL., Sweredoski MJ., Moradian A., Hess S., Kinney JB., Phillips R., 2018, Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria., Proc Natl Acad Sci U S A 115(21):E4796-E4805