RegulonDB RegulonDB 10.10: Regulon Form

PurR regulon in Escherichia coli K-12 genome

PurR Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional repressor PurR
Gene name(s): purR
Functional conformation(s): PurR-hypoxanthine , PurR-Guanine , PurR
Coregulator(s): CRP, DnaA, FNR, Fis, Fur, GadE, GcvA, IHF, Lrp, MarA, MetR, Nac, PepA, PhoP, PurR, RbsR, RutR
Summary: TF Summary ...[more]

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
PurR repressor    
Transcription Factor
Binding Sites
Functional conformation
Central Rel-Pos
PurR-hypoxanthine repressor purCp purC 2597772 2597787 2.5 ctgatatgatACGCAAACGTGTGCGTctgcaggaaa [GEA] [1]
PurR-hypoxanthine repressor purLp purL 2695620 2695635 -38.5 ttttatttccACGCAAACGGTTTCGTcagcgcatca [GEA] [1]
PurR-hypoxanthine repressor hflDp purB , hflD 1191787 1191802 892.5 tttgctgccgACGCAATCGGTTACCTtgatgcaatc [GEA] [1]
PurR-hypoxanthine repressor cvpAp1 ubiX , purF , cvpA 2430821 2430836 -28.5 aggaaatcccTACGCAAACGTTTTCTttttctgtta [GEA] [1]
PurR-hypoxanthine repressor purEp purK , purE 553172 553187 -37.5 tttcacagccACGCAACCGTTTTCCTtgctctcttt [GEA] [1]
PurR-hypoxanthine repressor glnBp2 glnB 2687476 2687491 20.5 cacgagctggATGCAAACGATTTCAAggaatgaatt [GEA] [8]
PurR-hypoxanthine repressor glnBp1 glnB 2687476 2687491 -42.5 cacgagctggATGCAAACGATTTCAAggaatgaatt [GEA] [8]
PurR-hypoxanthine repressor prsp prs 1262218 1262233 -48.5 aggttatcgcAAGAAAACGTTTTCGCgaggttgatg [GEA] [8]
PurR-hypoxanthine repressor speAp speB , speA 3086030 3086045 11.5 tgttatgaaaAAGAAACCGGTTGCGCagttggagcg [GEA] [8]
PurR-hypoxanthine repressor purTp purT 1930828 1930844 -21.5 ataaagacacACGCAAACGTTTTCGTTtatactgcgc [GEA] [9]

Evidence: [GEA] Gene expression analysis
Reference(s): [1] Cho BK., et al., 2011
[2] He B., et al., 1990
[3] Meng LM., et al., 1990
[4] He B., et al., 1992
[5] Rolfes RJ., et al., 1988
[6] Rolfes RJ., et al., 1988
[7] Watanabe W., et al., 1989
[8] He B., et al., 1993
[9] Belliveau NM., et al., 2018


 [1] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64

 [2] He B., Shiau A., Choi KY., Zalkin H., Smith JM., 1990, Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction., J Bacteriol 172(8):4555-62

 [3] Meng LM., Kilstrup M., Nygaard P., 1990, Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli., Eur J Biochem 187(2):373-9

 [4] He B., Smith JM., Zalkin H., 1992, Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR., J Bacteriol 174(1):130-6

 [5] Rolfes RJ., Zalkin H., 1988, Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene., J Biol Chem 263(36):19649-52

 [6] Rolfes RJ., Zalkin H., 1988, Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. Cloning, nucleotide sequence, and interaction with the purF operator., J Biol Chem 263(36):19653-61

 [7] Watanabe W., Sampei G., Aiba A., Mizobuchi K., 1989, Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis., J Bacteriol 171(1):198-204

 [8] He B., Choi KY., Zalkin H., 1993, Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor., J Bacteriol 175(11):3598-606

 [9] Belliveau NM., Barnes SL., Ireland WT., Jones DL., Sweredoski MJ., Moradian A., Hess S., Kinney JB., Phillips R., 2018, Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria., Proc Natl Acad Sci U S A 115(21):E4796-E4805