RegulonDB RegulonDB 11.0: Regulon Form
   
   


CRP,RhaR,RhaS regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
CRP Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB
Gene name(s): crp
Functional conformation(s): CRP , CRP-cyclic-AMP
Coregulator(s): AaeR, AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FrlR, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PlaR, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RspR, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YjjQ, ZraR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
RhaS Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class External sensing using transported metabolites
Synonym(s): DNA-binding transcriptional activator RhaS
Gene name(s): rhaS
Functional conformation(s): RhaS-α-L-rhamnopyranose , RhaS , RhaS-L-rhamnose
Coregulator(s): ArcA, CRP, RhaR, RhaS
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
RhaR      Tractordb tool
Connectivity class Local Regulator
Sensing Class External sensing using transported metabolites
Synonym(s): DNA-binding transcriptional activator RhaR
Gene name(s): rhaR
Functional conformation(s): RhaR-L-rhamnose , RhaR , RhaR-α-L-rhamnopyranose
Coregulator(s): CRP, RhaR, RhaS
Summary: TF Summary ...[more]

REGULATION EXERTED BY CRP,RhaR,RhaS Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
CRP activator RhaR activator RhaS activator RhaS unknown    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
CRP-cyclic-AMP activator rhaSp rhaS , rhaR 4097589 4097610 -111.5 tgaattgtggTGATGTGATGCTCACCGCATTTcctgaaaatt [GEA] [1]
[2]
CRP-cyclic-AMP activator rhaSp rhaS , rhaR 4097608 4097629 -92.5 gctcaccgcaTTTCCTGAAAATTCACGCTGTAtcttgaaaaa [GEA] [1]
RhaS-L-rhamnose activator rhaSp rhaS , rhaR 4097663 4097679 -40.0 cgtggttttcCGTCGAAAATTTAAGGTaagaacctga [GEA] [3]
[4]
RhaS-L-rhamnose activator rhaSp rhaS , rhaR 4097629 4097645 -74.0 ttcacgctgtATCTTGAAAAATCGACGttttttacgt [GEA] [3]
[4]
RhaS unknown rhaSp rhaS , rhaR 4098617 4098634 915.0 gcgaataatcAACTTCGTTCTCTGGCCGaggtagccac   [5]
RhaR-L-rhamnose activator rhaSp rhaS , rhaR 4097663 4097679 -40.0 cgtggttttcCGTCGAAAATTTAAGGTaagaacctga [GEA] [5]
RhaR-L-rhamnose activator rhaSp rhaS , rhaR 4097629 4097645 -74.0 ttcacgctgtATCTTGAAAAATCGACGttttttacgt [GEA] [3]
[5]
RhaR-L-rhamnose activator rhaSp2 rhaS , rhaR 4097663 4097679 -40.0 cgtggttttcCGTCGAAAATTTAAGGTaagaacctga [GEA] [5]
RhaS-L-rhamnose activator rhaSp2 rhaS , rhaR 4097629 4097645 -74.0 ttcacgctgtATCTTGAAAAATCGACGttttttacgt [GEA] [3]
[4]
RhaR-L-rhamnose activator rhaSp2 rhaS , rhaR 4097629 4097645 -74.0 ttcacgctgtATCTTGAAAAATCGACGttttttacgt [GEA] [3]
[5]
RhaS-L-rhamnose activator rhaSp2 rhaS , rhaR 4097663 4097679 -40.0 cgtggttttcCGTCGAAAATTTAAGGTaagaacctga [GEA] [3]
[4]
CRP-cyclic-AMP activator rhaSp2 rhaS , rhaR 4097589 4097610 -111.5 tgaattgtggTGATGTGATGCTCACCGCATTTcctgaaaatt [GEA] [1]
[2]
RhaS unknown rhaSp2 rhaS , rhaR 4098617 4098634 915.0 gcgaataatcAACTTCGTTCTCTGGCCGaggtagccac   [5]
CRP-cyclic-AMP activator rhaSp2 rhaS , rhaR 4097608 4097629 -92.5 gctcaccgcaTTTCCTGAAAATTCACGCTGTAtcttgaaaaa [GEA] [1]

Evidence: [GEA] Gene expression analysis
Reference(s): [1] Holcroft CC., et al., 2000
[2] Zheng D., et al., 2004
[3] Egan SM., et al., 1993
[4] Wickstrum JR., et al., 2010
[5] Wickstrum JR., et al., 2007



Reference(s)    

 [1] Holcroft CC., Egan SM., 2000, Interdependence of activation at rhaSR by cyclic AMP receptor protein, the RNA polymerase alpha subunit C-terminal domain, and rhaR., J Bacteriol 182(23):6774-82

 [2] Zheng D., Constantinidou C., Hobman JL., Minchin SD., 2004, Identification of the CRP regulon using in vitro and in vivo transcriptional profiling., Nucleic Acids Res 32(19):5874-93

 [3] Egan SM., Schleif RF., 1993, A regulatory cascade in the induction of rhaBAD., J Mol Biol 234(1):87-98

 [4] Wickstrum JR., Skredenske JM., Balasubramaniam V., Jones K., Egan SM., 2010, The AraC/XylS family activator RhaS negatively autoregulates rhaSR expression by preventing cyclic AMP receptor protein activation., J Bacteriol 192(1):225-32

 [5] Wickstrum JR., Skredenske JM., Kolin A., Jin DJ., Fang J., Egan SM., 2007, Transcription activation by the DNA-binding domain of the AraC family protein RhaS in the absence of its effector-binding domain., J Bacteriol 189(14):4984-93


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