RegulonDB RegulonDB 11.1: Regulon Form
   
   


DcuR,FNR,NarL regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
FNR Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional dual regulator FNR, NirA, NirR, OssA, OxrA
Gene name(s): fnr
Functional conformation(s): FNR , FNR-[4Fe-4S]2+ reduced , FNR-[2Fe-2S]2+ oxidized
Coregulator(s): AcrR, AdiY, ArcA, ArgP, ArgR, BtsR, CRP, CadC, CaiF, Cbl, CdaR, Cra, CueR, CusR, CysB, DcuR, DksA, DksA-ppGpp, DnaA, DpiA, EvgA, ExuR, FNR, FhlA, Fis, FlhDC, Fur, GadE, GadE-RcsB, GadW, GadX, GcvA, GlpR, H-NS, HdfR, HprR, HyfR, HypT, IHF, LeuO, Lrp, MalT, MarA, MetR, MlrA, ModE, Nac, NagC, NarL, NarP, NfeR, NikR, NorR, NrdR, NsrR, NtrC, OmpR, OxyR, PdhR, PhoB, PhoP, PurR, PuuR, RcsB, Rob, RstA, RutR, SlyA, SoxR, SoxS, TdcA, TdcR, TorR, YeiL, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
NarL Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class External-Two-component systems
Synonym(s): DNA-binding transcriptional dual regulator NarL, FrdR, NarR, nitrate/nitrite response regulator NarL
Gene name(s): narL
Functional conformation(s): NarL , NarL-phosphorylated
Coregulator(s): AppY, ArcA, CRP, Cra, DcuR, DpiA, EvgA, FNR, FhlA, Fis, FlhDC, Fur, H-NS, IHF, IscR, Lrp, ModE, NagC, NarL, NarP, NikR, NorR, NsrR, OxyR, PhoP, RcsB, RstA, SoxS, TorR, UvrY, YdeO
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
DcuR Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class External-Two-component systems
Synonym(s): DNA-binding transcriptional activator DcuR, DcuR response regulator, YjdG
Gene name(s): dcuR
Functional conformation(s): DcuR-phosphorylated , DcuR
Coregulator(s): ArcA, CRP, Cra, DcuR, FNR, NarL
Summary: TF Summary ...[more]

REGULATION EXERTED BY DcuR,FNR,NarL Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
DcuR activator FNR activator NarL repressor    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
NarL-phosphorylated repressor frdAp frdD , frdC , frdB , frdA 4382435 4382441 -26.0 atttcagactTATCCATcagactatac   [1]
NarL-phosphorylated repressor frdAp frdD , frdC , frdB , frdA 4382421 4382427 -12.0 catcagactaTACTGTTgtacctataa   [1]
NarL-phosphorylated repressor frdAp frdD , frdC , frdB , frdA 4382396 4382402 14.0 aaaggagcagTGGAATAgcgttcgcag   [1]
NarL-phosphorylated repressor frdAp frdD , frdC , frdB , frdA 4382404 4382410 6.0 gtacctataaAGGAGCAgtggaatagc   [1]
NarL-phosphorylated repressor frdAp frdD , frdC , frdB , frdA 4382413 4382419 -4.0 tatactgttgTACCTATaaaggagcag   [1]
DcuR-phosphorylated activator frdAp frdD , frdC , frdB , frdA 4382508 4382524 -104.0 cgatctgatgGTTTAGTAATTAAATTAatcatcttca [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [2]
[3]
[4]
[5]
FNR activator frdAp frdD , frdC , frdB , frdA 4382451 4382464 -45.5 actaaaaaaaTCGATCTCGTCAAAtttcagactt [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [2]
[6]
[7]
[8]
NarL-phosphorylated repressor frdAp2 frdD , frdC , frdB , frdA 4382421 4382427 -12.0 catcagactaTACTGTTgtacctataa   [1]
NarL-phosphorylated repressor frdAp2 frdD , frdC , frdB , frdA 4382396 4382402 14.0 aaaggagcagTGGAATAgcgttcgcag   [1]
NarL-phosphorylated repressor frdAp2 frdD , frdC , frdB , frdA 4382435 4382441 -26.0 atttcagactTATCCATcagactatac   [1]
DcuR-phosphorylated activator frdAp2 frdD , frdC , frdB , frdA 4382508 4382524 -104.0 cgatctgatgGTTTAGTAATTAAATTAatcatcttca [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [2]
[3]
[4]
[5]
NarL-phosphorylated repressor frdAp2 frdD , frdC , frdB , frdA 4382404 4382410 6.0 gtacctataaAGGAGCAgtggaatagc   [1]
NarL-phosphorylated repressor frdAp2 frdD , frdC , frdB , frdA 4382413 4382419 -4.0 tatactgttgTACCTATaaaggagcag   [1]
FNR activator frdAp2 frdD , frdC , frdB , frdA 4382451 4382464 -45.5 actaaaaaaaTCGATCTCGTCAAAtttcagactt [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [2]
[6]
[7]
[8]

Evidence: [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
Reference(s): [1] Li J., et al., 1994
[2] Abo-Amer AE., et al., 2004
[3] Golby P., et al., 1999
[4] Janausch IG., et al., 2004
[5] Zientz E., et al., 1998
[6] Jones HM., et al., 1987
[7] Shalel-Levanon S., et al., 2005
[8] Tyson KL., et al., 1993



Reference(s)    

 [1] Li J., Kustu S., Stewart V., 1994, In vitro interaction of nitrate-responsive regulatory protein NarL with DNA target sequences in the fdnG, narG, narK and frdA operon control regions of Escherichia coli K-12., J Mol Biol 241(2):150-65

 [2] Abo-Amer AE., Munn J., Jackson K., Aktas M., Golby P., Kelly DJ., Andrews SC., 2004, DNA interaction and phosphotransfer of the C4-dicarboxylate-responsive DcuS-DcuR two-component regulatory system from Escherichia coli., J Bacteriol 186(6):1879-89

 [3] Golby P., Davies S., Kelly DJ., Guest JR., Andrews SC., 1999, Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-dicarboxylates in Escherichia coli., J Bacteriol 181(4):1238-48

 [4] Janausch IG., Garcia-Moreno I., Lehnen D., Zeuner Y., Unden G., 2004, Phosphorylation and DNA binding of the regulator DcuR of the fumarate-responsive two-component system DcuSR of Escherichia coli., Microbiology 150(Pt 4):877-83

 [5] Zientz E., Bongaerts J., Unden G., 1998, Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system., J Bacteriol 180(20):5421-5

 [6] Jones HM., Gunsalus RP., 1987, Regulation of Escherichia coli fumarate reductase (frdABCD) operon expression by respiratory electron acceptors and the fnr gene product., J Bacteriol 169(7):3340-9

 [7] Shalel-Levanon S., San KY., Bennett GN., 2005, Effect of ArcA and FNR on the expression of genes related to the oxygen regulation and the glycolysis pathway in Escherichia coli under microaerobic growth conditions., Biotechnol Bioeng 92(2):147-59

 [8] Tyson KL., Bell AI., Cole JA., Busby SJ., 1993, Definition of nitrite and nitrate response elements at the anaerobically inducible Escherichia coli nirB promoter: interactions between FNR and NarL., Mol Microbiol 7(1):151-7

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