RegulonDB

TRANSCRIPTION FACTOR:

Name:

CRP	Weight Matrix Tractordb tool

Connectivity class

Global Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB

Gene name(s):

crp

Functional conformation(s):

CRP , CRP-cyclic-AMP

Coregulator(s):

AaeR, AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FrlR, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PlaR, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RspR, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YjjQ, ZraR, ppGpp

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Info

Name:

MelR	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

Sensing external and internal signals

Synonym(s):

DNA-binding transcriptional dual regulator MelR

Gene name(s):

melR

Functional conformation(s):

MelR , MelR-melibiose

Coregulator(s):

CRP, MelR

Summary:

TF Summary ...[more]

REGULATION EXERTED BY CRP,MelR

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
CRP activator MelR dual
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
MelR-melibiose	activator	melRp	melR	4341836	4341853	-194.5	tgcagaaaacCTCCGGCTTCCAGGTTGAgcattcccat	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1]
CRP-cyclic-AMP	activator	melRp	melR	4341795	4341816	-155.5	taaactcagaTCCTGCGTGAAGCAGCAGTAAAtctgagttta	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [3] [1] [4]
CRP-cyclic-AMP	activator	melRp	melR	4341681	4341702	-41.5	agagggtgaaAACCGTGCTCCCACTCGCAGTCatcctccctc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [3] [1] [4] [5]
MelR-melibiose	repressor	melRp	melR	4341778	4341795	-136.5	gcagcagtaaATCTGAGTTTATGGGAATaaattacctg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [6] [7] [1]
MelR-melibiose	repressor	melRp	melR	4341758	4341775	-116.5	atgggaataaATTACCTGAAAAGCAGAGttttcggcct	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [6] [7] [1]
MelR	repressor	melRp	melR	4341816	4341833	-174.5	caggttgagcATTCCCATAAACTCAGATcctgcgtgaa	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [3] [7] [1]
MelR	repressor	melRp	melR	4341640	4341657	2.5	cctgccataaTTCTGATATTCCAGGAAAgagagccatc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [3] [7]
CRP-cyclic-AMP	activator	melAp	melA , melB	4341681	4341702	-195.5	gagggaggatGACTGCGAGTGGGAGCACGGTTttcaccctct	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [3] [1] [5]
MelR-melibiose	activator	melAp	melA , melB	4341758	4341775	-120.5	aggccgaaaaCTCTGCTTTTCAGGTAATttattcccat	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [1] [4]
MelR-melibiose	activator	melAp	melA , melB	4341778	4341795	-100.5	caggtaatttATTCCCATAAACTCAGATttactgctgc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [1] [4]
MelR-melibiose	activator	melAp	melA , melB	4341816	4341833	-62.5	ttcacgcaggATCTGAGTTTATGGGAATgctcaacctg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [8] [3] [9] [1]
CRP-cyclic-AMP	activator	melAp	melA , melB	4341795	4341816	-81.5	taaactcagaTTTACTGCTGCTTCACGCAGGAtctgagttta	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[3] [1] [4]
MelR-melibiose	activator	melAp	melA , melB	4341836	4341853	-42.5	atgggaatgcTCAACCTGGAAGCCGGAGgttttctgca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [8] [3] [9] [1]
MelR	repressor	melAp	melA , melB	4341640	4341657	-238.5	gatggctctcTTTCCTGGAATATCAGAAttatggcagg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [3]
MelR	repressor	melAp	melA , melB	4341816	4341833	-62.5	ttcacgcaggATCTGAGTTTATGGGAATgctcaacctg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [8] [3] [9] [1]

Evidence:	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
Reference(s):	[1] Wade JT., et al., 2000 [2] Belyaeva TA., et al., 2000 [3] Kahramanoglou C., et al., 2006 [4] Wade JT., et al., 2001 [5] Zheng D., et al., 2004 [6] Howard VJ., et al., 2002 [7] Samarasinghe S., et al., 2008 [8] Grainger DC., et al., 2003 [9] Tamai E., et al., 2000

Reference(s)
[1] Wade JT., Belyaeva TA., Hyde EI., Busby SJ., 2000, Repression of the Escherichia coli melR promoter by MelR: evidence that efficient repression requires the formation of a repression loop., Mol Microbiol 36(1):223-9
[2] Belyaeva TA., Wade JT., Webster CL., Howard VJ., Thomas MS., Hyde EI., Busby SJ., 2000, Transcription activation at the Escherichia coli melAB promoter: the role of MelR and the cyclic AMP receptor protein., Mol Microbiol 36(1):211-22
[3] Kahramanoglou C., Webster CL., El-Robh MS., Belyaeva TA., Busby SJ., 2006, Mutational analysis of the Escherichia coli melR gene suggests a two-state concerted model to explain transcriptional activation and repression in the melibiose operon., J Bacteriol 188(9):3199-207
[4] Wade JT., Belyaeva TA., Hyde EI., Busby SJ., 2001, A simple mechanism for co-dependence on two activators at an Escherichia coli promoter., EMBO J 20(24):7160-7
[5] Zheng D., Constantinidou C., Hobman JL., Minchin SD., 2004, Identification of the CRP regulon using in vitro and in vivo transcriptional profiling., Nucleic Acids Res 32(19):5874-93
[6] Howard VJ., Belyaeva TA., Busby SJ., Hyde EI., 2002, DNA binding of the transcription activator protein MelR from Escherichia coli and its C-terminal domain., Nucleic Acids Res 30(12):2692-700
[7] Samarasinghe S., El-Robh MS., Grainger DC., Zhang W., Soultanas P., Busby SJ., 2008, Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR., Nucleic Acids Res 36(8):2667-76
[8] Grainger DC., Belyaeva TA., Lee DJ., Hyde EI., Busby SJ., 2003, Binding of the Escherichia coli MelR protein to the melAB promoter: orientation of MelR subunits and investigation of MelR-DNA contacts., Mol Microbiol 48(2):335-48
[9] Tamai E., Belyaeva TA., Busby SJ., Tsuchiya T., 2000, Mutations that increase the activity of the promoter of the Escherichia coli melibiose operon improve the binding of MelR, a transcription activator triggered by melibiose., J Biol Chem 275(22):17058-63